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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados; Embrapa Unidades Centrais. |
Data corrente: |
05/11/2010 |
Data da última atualização: |
01/03/2013 |
Tipo da produção científica: |
Documentos |
Autoria: |
RAMOS, S. Y. |
Afiliação: |
SIMONE YURI RAMOS, CPAC. |
Título: |
Panorama da política agrícola brasileira: a política de garantia de preços mínimos. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Planaltina, DF: Embrapa Cerrados, 2009. |
Páginas: |
84 p. |
Série: |
(Embrapa Cerrados. Documentos, 262) |
Idioma: |
Português |
Conteúdo: |
Informações sobre a política agrícola brasileira, notadamente no que tange à Política de Garantia de Preços Mínimos e ao Programa de Estímulo à Produção Agropecuária sustentável, com a finalidade de embasar pesquisas futuras que possam dar suporte à formulação e avaliação de programas voltados à agropecuária. |
Palavras-Chave: |
Economy; Integração lavoura-pecuária. |
Thesagro: |
Agricultura; Agronegócio; Economia. |
Thesaurus Nal: |
Agribusiness; Agriculture. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/77808/1/doc-262.pdf
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Marc: |
LEADER 00943nam a2200217 a 4500 001 1866143 005 2013-03-01 008 2009 bl uuuu 00u1 u #d 100 1 $aRAMOS, S. Y. 245 $aPanorama da política agrícola brasileira$ba política de garantia de preços mínimos. 260 $aPlanaltina, DF: Embrapa Cerrados$c2009 300 $a84 p. 490 $a(Embrapa Cerrados. Documentos, 262) 520 $aInformações sobre a política agrícola brasileira, notadamente no que tange à Política de Garantia de Preços Mínimos e ao Programa de Estímulo à Produção Agropecuária sustentável, com a finalidade de embasar pesquisas futuras que possam dar suporte à formulação e avaliação de programas voltados à agropecuária. 650 $aAgribusiness 650 $aAgriculture 650 $aAgricultura 650 $aAgronegócio 650 $aEconomia 653 $aEconomy 653 $aIntegração lavoura-pecuária
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Registro original: |
Embrapa Cerrados (CPAC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
25/11/2015 |
Data da última atualização: |
04/03/2016 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
LOUZAN, A. L. R. M.; CAITANO, M. A. B.; SANTOS, M. G. dos; VERBISCK, N. V.; ROSINHA, G. M. S. |
Afiliação: |
UFMS; UFMS; MARIA GORETTI DOS SANTOS, CNPGC; NEWTON VALERIO VERBISCK, CNPGC; GRACIA MARIA SOARES ROSINHA, CNPGC. |
Título: |
Maldi-tof mass spectrometry identification and differenteiation of Brucella species. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: MEDITERRANEAN SEA REGION COUNTRIES MASS SPECTROMETRY WORKSHOP (MEDMS III), 3., 2015., Athens, Greece. [Poster]. Athens, Greece: Univeristy of Athens, 2015. p. 61. |
Idioma: |
Inglês |
Conteúdo: |
Brucella species are Gram-negative bacteria that cause BrucelIosis, also known as Mediterranean or Malta fever, a highly contagious and chronic disease that occurs worldwide. Brucellosis may affect human after close contact with infected animal secretions and by ingestion of contaminated animal products, such as those made with unpausterized milk or undercooked meat. Livestock, including cattle, sheep, goats, pigs and dogs are the most common reservoirs for Brucella bacteria, which the detection stilI relies on microbiological culture and serological tests. However, in the last decade, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI- TOF MS) has been used for microrganism identification at genus and species leveI. CurrentIy, MALDI-TOF MS is being used as a rapid, cost effective and reliable methodology for bacteria identification even at the subspecies leveI. Here we show that MALDI- TOF MS is useful for Brucella identification and differentiation, B. abortus (strains 544, S2308, 19 and RB51), B. suis, B. ovis and B. canis reference samples were grown and, after ethanol inactivation, proteins were extracted with formic acidlacetonitrile. Mass spectra were acquired with alpha-cyano-4-hydroxy cinnamic acid in an Autoflex III Smartbeam mass spectrometer (Bruker Daltonics). MALDI Biotyper software (Bruker Daltonics) dendogram analysis provided that the mass spectra profiles (MSPs) obtained can distinguish alI the species of Brucella studied herein. Moreover, fingerprints of the wild-type S2308 and vaccine 19 and RB51 B. abortus strains are clearly different. The next step wilI be to use these MSPs to detect and identify Brucella from infected cattle. Financial support: Embrapa, FUNDECT, CNPq and CAPES. MenosBrucella species are Gram-negative bacteria that cause BrucelIosis, also known as Mediterranean or Malta fever, a highly contagious and chronic disease that occurs worldwide. Brucellosis may affect human after close contact with infected animal secretions and by ingestion of contaminated animal products, such as those made with unpausterized milk or undercooked meat. Livestock, including cattle, sheep, goats, pigs and dogs are the most common reservoirs for Brucella bacteria, which the detection stilI relies on microbiological culture and serological tests. However, in the last decade, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI- TOF MS) has been used for microrganism identification at genus and species leveI. CurrentIy, MALDI-TOF MS is being used as a rapid, cost effective and reliable methodology for bacteria identification even at the subspecies leveI. Here we show that MALDI- TOF MS is useful for Brucella identification and differentiation, B. abortus (strains 544, S2308, 19 and RB51), B. suis, B. ovis and B. canis reference samples were grown and, after ethanol inactivation, proteins were extracted with formic acidlacetonitrile. Mass spectra were acquired with alpha-cyano-4-hydroxy cinnamic acid in an Autoflex III Smartbeam mass spectrometer (Bruker Daltonics). MALDI Biotyper software (Bruker Daltonics) dendogram analysis provided that the mass spectra profiles (MSPs) obtained can distinguish alI the species of Brucella studied he... Mostrar Tudo |
Thesagro: |
Brucella. |
Thesaurus NAL: |
Brucellosis; Spectroscopy. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02467nam a2200193 a 4500 001 2029543 005 2016-03-04 008 2015 bl uuuu u00u1 u #d 100 1 $aLOUZAN, A. L. R. M. 245 $aMaldi-tof mass spectrometry identification and differenteiation of Brucella species.$h[electronic resource] 260 $aIn: MEDITERRANEAN SEA REGION COUNTRIES MASS SPECTROMETRY WORKSHOP (MEDMS III), 3., 2015., Athens, Greece. [Poster]. Athens, Greece: Univeristy of Athens, 2015. p. 61.$c2015 520 $aBrucella species are Gram-negative bacteria that cause BrucelIosis, also known as Mediterranean or Malta fever, a highly contagious and chronic disease that occurs worldwide. Brucellosis may affect human after close contact with infected animal secretions and by ingestion of contaminated animal products, such as those made with unpausterized milk or undercooked meat. Livestock, including cattle, sheep, goats, pigs and dogs are the most common reservoirs for Brucella bacteria, which the detection stilI relies on microbiological culture and serological tests. However, in the last decade, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI- TOF MS) has been used for microrganism identification at genus and species leveI. CurrentIy, MALDI-TOF MS is being used as a rapid, cost effective and reliable methodology for bacteria identification even at the subspecies leveI. Here we show that MALDI- TOF MS is useful for Brucella identification and differentiation, B. abortus (strains 544, S2308, 19 and RB51), B. suis, B. ovis and B. canis reference samples were grown and, after ethanol inactivation, proteins were extracted with formic acidlacetonitrile. Mass spectra were acquired with alpha-cyano-4-hydroxy cinnamic acid in an Autoflex III Smartbeam mass spectrometer (Bruker Daltonics). MALDI Biotyper software (Bruker Daltonics) dendogram analysis provided that the mass spectra profiles (MSPs) obtained can distinguish alI the species of Brucella studied herein. Moreover, fingerprints of the wild-type S2308 and vaccine 19 and RB51 B. abortus strains are clearly different. The next step wilI be to use these MSPs to detect and identify Brucella from infected cattle. Financial support: Embrapa, FUNDECT, CNPq and CAPES. 650 $aBrucellosis 650 $aSpectroscopy 650 $aBrucella 700 1 $aCAITANO, M. A. B. 700 1 $aSANTOS, M. G. dos 700 1 $aVERBISCK, N. V. 700 1 $aROSINHA, G. M. S.
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