Data/hora: 03/06/2024 10:53:06

Biblioteca(s): | Embrapa Pecuária Sudeste.
Data corrente: | 23/01/2006
Data da última atualização: | 13/03/2023
Autoria: | PARRA, A.; RAMON, M; ALONSO, J.; LEMOS, S. G.; VIEIRA, E.; NOGUEIRA, A. R. de A.
Afiliação: | ALEIX PARRA, Universitat Autonoma de Barcelona; MERITXELL R AMON, Universitat Autonoma de Barcelona; JULIAN ALONSO, Universitat Autonoma de Barcelona; SHERLAN G. LEMOS, Universidade Federal de Sao Carlos; EDIVAN C. VIEIRA, Universidade Federal de Sao Carlos; ANA RITA DE ARAUJO NOGUEIRA, CPPSE.
Título: | Flow injection potentiometric system for the simultaneous determination of inositol phosphates and phosphate: phosphorus nutritional evaluation on seeds and grains.
Ano de publicação: | 2005
Fonte/Imprenta: | Journal of Agricultural and Food Chemistry, v. 53, p. 7644-7648, 2005.
DOI: | 10.1021/jf050226r
Idioma: | Inglês
Conteúdo: | A simple flow injection potentiometric (FIP) system, which uses a tubular cobalt electrode, has been developed for phosphorus nutritional evaluation of seeds and grains. Inorganic phosphorus, Pi, is determined using a 1 × 10-2 mol?L-1 potassium phthalate buffer solution adjusted at pH 4. A sensitivity of 47 mV/decade and an operating range from 10 to 1000 mg?L-1 (1 × 10-4-1 × 10-2 M) of dihydrogen phosphate are obtained. The inositol phosphates amount, which is referred to the organic phosphorus, Porg, is directly determined from extracts using a 1 × 10-2 mol?L-1 Tris-HCl buffer solution adjusted at pH 8. A sensitivity of 127 mV/decade and an operating range of 10-1000 mg?L-1 (2.5 × 10-4-5 × 10-3 M) of Porg (expressed as inositol hexakisphosphoric acid monocalcium) are achieved. Some samples of seed and grain are analyzed by an ICP-OES and a spectrophotometric method to compare results to the developed flow system; no significant differences at the 95% confidence level are observed using a paired t test. Other samples such as animal nursing feed, soybean meal, and corn are also analyzed with the proposed FIP system, showing a good correlation to the ICP-OES values.
Palavras-Chave: | Flow infection; Potentiometric system.
Categoria do assunto: | W Química e Física
Registro original: | Embrapa Pecuária Sudeste (CPPSE)
Exemplares: | CPPSE - (15981-1) - UPC - AP - DD - PROCI-2005.00189 - PAR - 2005.00189 - DIS