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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
11/09/1997 |
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Data da última atualização: |
11/09/1997 |
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Autoria: |
SILVA, R. A. M. S.; RAMIREZ, L.; DAVILA, A. M. R.; VICTORIO, A. M.; PEREIRA, M. E. B. |
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Afiliação: |
EMBRAPA. Centro de Pesquisa Agropecuaria do Pantanal (Corumba, MS); UFMS. Centro Universitario de Corumba. Departamento de Ciencias do Ambiente (Corumba, MS). |
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Título: |
An investigation of in vitro phagocytosis of Trypanosoma evansi by rat peritoneal macrophages: increase mediated by parasite-specific antibodies. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Memorias do Instituto Oswaldo Cruz, Rio de Janeiro, v.91, p.105, 1996. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Trypanosoma evansi causes and usually fatal disease in rats, mice, horses and dogs. Phagocytosis and the intracellular killing of pathogens in probaly one of the most important defense system available to an animal. Macrophages (Mo) are central cells in the action and regulation of the immune network. This is in view of their function as phagocytic and antigen-presenting cells, are their release of mediators, expression of surface receptors, and interaction with T and B cells. This experiment was developed to evaluate the influence of parasitic-specific antibody in the phagocytosis by peritoneal Mo of rats. Peritoneal Mo, 1,45 x 10 6 were isolated from Wistar rats pretreated 3 days before single intraperitoneal dose of tioglicolate. The Mo were then precultivated for 24 h. on coverslips in culture flasks. Trypanosomes, 8,7 x 10 6(final concentration) were added to the adherent macrophages and were cocultivated at 37 oC for 5, 15, 30, 45 min. in EAGLE medium (Instituto Adolfo Lutz, SP). The trypanosome/ Mo proportion was 6:1. The phagocytosis test was performed in two groups: group A (Mo treated with normal serum) and B (Mo treated with 200 ul of serum from horse naturally infected by T.evansi). The parasites were not observed attached or phagocytosed by Mo in group A. In group B trypanosomes attached to 51,26% of the Mos within 5 min, 17,20% within 15 min, 46,15% within 30 min, 10,65% within 45 min. The phagocytosis process was observed in 96,63% of the Mos at 5 min, 83,87% at 15 min, 100% at 30 min, and 94,26% at 45 min. We conclude that the absence of phagocytosis in the group A could be explained normal macrophages have some difficulty to uptake the parasites; although some of parasites attach to the Mo, they are not internalized as observed in experiments with T.brucei. The espace from phagocytosis is even improved because of short cell contacts due to permanent movement of the cells concerned. However, in the presence of parasite-specific antibodies (group B), internalization and degradationof the trypanosomes took place. So, according to Shakibaei & Frevert, in the absence of antibodies, bloodstream trypanosomes are protected against phagocytosis by the surface coat. In immune animals the parasites are removed from the bloodsteam by phagocytic cells. The opsonization with specific antibodies or proteolytic removal of the coat result in rapid uptake of the parasites by Mos. MenosTrypanosoma evansi causes and usually fatal disease in rats, mice, horses and dogs. Phagocytosis and the intracellular killing of pathogens in probaly one of the most important defense system available to an animal. Macrophages (Mo) are central cells in the action and regulation of the immune network. This is in view of their function as phagocytic and antigen-presenting cells, are their release of mediators, expression of surface receptors, and interaction with T and B cells. This experiment was developed to evaluate the influence of parasitic-specific antibody in the phagocytosis by peritoneal Mo of rats. Peritoneal Mo, 1,45 x 10 6 were isolated from Wistar rats pretreated 3 days before single intraperitoneal dose of tioglicolate. The Mo were then precultivated for 24 h. on coverslips in culture flasks. Trypanosomes, 8,7 x 10 6(final concentration) were added to the adherent macrophages and were cocultivated at 37 oC for 5, 15, 30, 45 min. in EAGLE medium (Instituto Adolfo Lutz, SP). The trypanosome/ Mo proportion was 6:1. The phagocytosis test was performed in two groups: group A (Mo treated with normal serum) and B (Mo treated with 200 ul of serum from horse naturally infected by T.evansi). The parasites were not observed attached or phagocytosed by Mo in group A. In group B trypanosomes attached to 51,26% of the Mos within 5 min, 17,20% within 15 min, 46,15% within 30 min, 10,65% within 45 min. The phagocytosis process was observed in 96,63% of the Mos at 5 min, 83,87%... Mostrar Tudo |
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Thesaurus Nal: |
Trypanosoma evansi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03040naa a2200181 a 4500
001 1791630
005 1997-09-11
008 1996 bl uuuu u00u1 u #d
100 1 $aSILVA, R. A. M. S.
245 $aAn investigation of in vitro phagocytosis of Trypanosoma evansi by rat peritoneal macrophages$bincrease mediated by parasite-specific antibodies.
260 $c1996
520 $aTrypanosoma evansi causes and usually fatal disease in rats, mice, horses and dogs. Phagocytosis and the intracellular killing of pathogens in probaly one of the most important defense system available to an animal. Macrophages (Mo) are central cells in the action and regulation of the immune network. This is in view of their function as phagocytic and antigen-presenting cells, are their release of mediators, expression of surface receptors, and interaction with T and B cells. This experiment was developed to evaluate the influence of parasitic-specific antibody in the phagocytosis by peritoneal Mo of rats. Peritoneal Mo, 1,45 x 10 6 were isolated from Wistar rats pretreated 3 days before single intraperitoneal dose of tioglicolate. The Mo were then precultivated for 24 h. on coverslips in culture flasks. Trypanosomes, 8,7 x 10 6(final concentration) were added to the adherent macrophages and were cocultivated at 37 oC for 5, 15, 30, 45 min. in EAGLE medium (Instituto Adolfo Lutz, SP). The trypanosome/ Mo proportion was 6:1. The phagocytosis test was performed in two groups: group A (Mo treated with normal serum) and B (Mo treated with 200 ul of serum from horse naturally infected by T.evansi). The parasites were not observed attached or phagocytosed by Mo in group A. In group B trypanosomes attached to 51,26% of the Mos within 5 min, 17,20% within 15 min, 46,15% within 30 min, 10,65% within 45 min. The phagocytosis process was observed in 96,63% of the Mos at 5 min, 83,87% at 15 min, 100% at 30 min, and 94,26% at 45 min. We conclude that the absence of phagocytosis in the group A could be explained normal macrophages have some difficulty to uptake the parasites; although some of parasites attach to the Mo, they are not internalized as observed in experiments with T.brucei. The espace from phagocytosis is even improved because of short cell contacts due to permanent movement of the cells concerned. However, in the presence of parasite-specific antibodies (group B), internalization and degradationof the trypanosomes took place. So, according to Shakibaei & Frevert, in the absence of antibodies, bloodstream trypanosomes are protected against phagocytosis by the surface coat. In immune animals the parasites are removed from the bloodsteam by phagocytic cells. The opsonization with specific antibodies or proteolytic removal of the coat result in rapid uptake of the parasites by Mos.
650 $aTrypanosoma evansi
700 1 $aRAMIREZ, L.
700 1 $aDAVILA, A. M. R.
700 1 $aVICTORIO, A. M.
700 1 $aPEREIRA, M. E. B.
773 $tMemorias do Instituto Oswaldo Cruz, Rio de Janeiro$gv.91, p.105, 1996. Suplemento.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
08/01/2018 |
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Data da última atualização: |
12/12/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
RAMOS, R. R. D.; MOURA, M. S. B. de; SILVA, T. G. F. da; GALVINCIO, J. D. |
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Afiliação: |
RICARDO RIVELINO DANTAS RAMOS, UNIVASF; MAGNA SOELMA BESERRA DE MOURA, CPATSA; THIERES GEORGE FREIRE DA SILVA, Professor, UAST/UFRPE, Serra Talhada, PE; JOSICLEDA DOMICIANO GALVÍNCIO, Professora da UFPE. |
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Título: |
Evapotranspiração da cana-de-açúcar utilizando o Simple Algorithm for Evapotranspiration Retrieving (SAFER) no Vale São Francisco. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 20; SIMPÓSIO DE MUDANÇAS CLIMÁTICAS E DESERTIFICAÇÃO NO SEMIÁRIDO BRASILEIRO, 5., 2017, Juazeiro, BA. A agrometeorologia na solução de problemas multiescala: anais. Petrolina: Embrapa Semiárido; Juazeiro: UNIVASF; Campinas: Sociedade Brasileira de Agrometeorologia, 2017. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi aplicar o Simple Algorithm For Evapotranspiration Retrieving (SAFER) em imagens Landsat 8 para determinação da evapotranspiração real (ETr) da cana-de-açúcar irrigada no Submédio do Vale São Francisco. Para isso, selecionou-se uma área comercial de cana-de-açúcar na Usina Agrovale, em Juazeiro, BA. Foram utilizadas imagens do satélite Landsat 8 (OLI/TIRS) para os dias 27 de outubro de 2015 e 22 de maio de 2016, quando a cana-de-açúcar estava em diferentes estágios de desenvolvimento. Os valores da ETr foram determinados por meio do algoritmo SAFER, utilizando o albedo (?s), temperatura da superfície (Ts) e IVDN (Índice de Vegetação por Diferença Normalizada). Para comparação com dados de imagens de satélite, a ETr foi medidas em três áreas de canade- açúcar utilizando-se o método do balanço de energia com base na Razão de Bowen. Observou-se que o uso do SAFER permite boas estimativas da evapotranspiração real da cana-de-açúcar, respondendo às diferentes fases fenológicas e sistemas de manjo do cultivo irrigado no Submédio do São Francisco. |
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Palavras-Chave: |
Cana-de-açúcar; Razão de Bowen; SAFER; Simple Algorithm For Evapotranspiration Retrieving. |
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Thesagro: |
Balanço de Energia; Evapotranspiração; Sensoriamento remoto. |
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Thesaurus NAL: |
Remote sensing; Sugarcane. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177288/1/Magna-11.pdf
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Marc: |
LEADER 02237nam a2200265 a 4500
001 2084468
005 2023-12-12
008 2017 bl uuuu u00u1 u #d
100 1 $aRAMOS, R. R. D.
245 $aEvapotranspiração da cana-de-açúcar utilizando o Simple Algorithm for Evapotranspiration Retrieving (SAFER) no Vale São Francisco.
260 $aIn: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 20; SIMPÓSIO DE MUDANÇAS CLIMÁTICAS E DESERTIFICAÇÃO NO SEMIÁRIDO BRASILEIRO, 5., 2017, Juazeiro, BA. A agrometeorologia na solução de problemas multiescala: anais. Petrolina: Embrapa Semiárido; Juazeiro: UNIVASF; Campinas: Sociedade Brasileira de Agrometeorologia$c2017
300 $c1 CD-ROM.
520 $aO objetivo deste trabalho foi aplicar o Simple Algorithm For Evapotranspiration Retrieving (SAFER) em imagens Landsat 8 para determinação da evapotranspiração real (ETr) da cana-de-açúcar irrigada no Submédio do Vale São Francisco. Para isso, selecionou-se uma área comercial de cana-de-açúcar na Usina Agrovale, em Juazeiro, BA. Foram utilizadas imagens do satélite Landsat 8 (OLI/TIRS) para os dias 27 de outubro de 2015 e 22 de maio de 2016, quando a cana-de-açúcar estava em diferentes estágios de desenvolvimento. Os valores da ETr foram determinados por meio do algoritmo SAFER, utilizando o albedo (?s), temperatura da superfície (Ts) e IVDN (Índice de Vegetação por Diferença Normalizada). Para comparação com dados de imagens de satélite, a ETr foi medidas em três áreas de canade- açúcar utilizando-se o método do balanço de energia com base na Razão de Bowen. Observou-se que o uso do SAFER permite boas estimativas da evapotranspiração real da cana-de-açúcar, respondendo às diferentes fases fenológicas e sistemas de manjo do cultivo irrigado no Submédio do São Francisco.
650 $aRemote sensing
650 $aSugarcane
650 $aBalanço de Energia
650 $aEvapotranspiração
650 $aSensoriamento remoto
653 $aCana-de-açúcar
653 $aRazão de Bowen
653 $aSAFER
653 $aSimple Algorithm For Evapotranspiration Retrieving
700 1 $aMOURA, M. S. B. de
700 1 $aSILVA, T. G. F. da
700 1 $aGALVINCIO, J. D.
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