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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
26/12/2019 |
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Data da última atualização: |
30/12/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
RAISER, A. L.; SILVA, B. R. da; MORALES, M. M. |
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Afiliação: |
ALÉXIA LORENZI RAISER, UFMT-SINOP; BRUNO RAFAEL DA SILVA, CPAMT; MARINA MOURA MORALES, CNPF. |
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Título: |
Avaliação do método de extração para análise de HPAs em amostras de solo e biocarvão por GC-MS. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: Encontro de Ciência e Tecnologias Agrossustentávies; Jornada Científica da Embrapa Agrossilvipastoril, 6., 2017, Sinop, MT. Resumos... Sinop, MT: Embrapa Agrossilpastoril, 2018. p. 213-216. |
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Série: |
(Embrapa Agrossilvipastoril. Eventos Técnicos & Científicos, 1) |
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Idioma: |
Português |
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Conteúdo: |
Os hidrocarbonetos policíclicos aromáticos (HPAs) são compostos orgânicos semi-voláteis formados principalmente pela combustão incompleta de materiais orgânicos (Grover et al., 2013; Liu et al., 2015), sendo listados como poluentes pela União Européia e pela Agência de Proteção Ambiental dos EUA em solos (Xue et al., 2015). No processo de produção de biocarvão, durante a pirólise, ocorre a formação de HPAs devido aos fragmentos instáveis gerados (Hale et al., 2012). O método tradicional de extração de HPAs é realizado através do Soxhlet (United States, 1996). Em comparação com outros métodos de extração, o Soxhlet apresenta desvantagens como maior tempo despendido, grande quantidade de solvente e como consequência, problemas ambientais (Castro et al., 2010). O método de extração por ultrassom apresenta vantagens como simplicidade, menor tempo de extração e de baixo volume de solventes, diminuindo os possíveis danos ao ambiente (Cardoso et al., 2014). Dessa forma, nosso objetivo foi comparar os métodos de extração, Soxhlet e ultrassom, de acordo com o percentual de recuperação de HPAs em amostras de biocarvão (BC) e mistura de solo/biocarvão (SBC) por cromatografia gasosa. |
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Palavras-Chave: |
Análise de HPAs; Análise de Solo; Biocarvão. |
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Thesagro: |
Amostragem; Solo. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207968/1/2019-RAC-Marina-ECTA-Avaliacao-do-metodo-de-extracao.pdf
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Marc: |
LEADER 02049nam a2200205 a 4500
001 2117667
005 2019-12-30
008 2018 bl uuuu u00u1 u #d
100 1 $aRAISER, A. L.
245 $aAvaliação do método de extração para análise de HPAs em amostras de solo e biocarvão por GC-MS.$h[electronic resource]
260 $aIn: Encontro de Ciência e Tecnologias Agrossustentávies; Jornada Científica da Embrapa Agrossilvipastoril, 6., 2017, Sinop, MT. Resumos... Sinop, MT: Embrapa Agrossilpastoril, 2018. p. 213-216.$c2018
490 $a(Embrapa Agrossilvipastoril. Eventos Técnicos & Científicos, 1)
520 $aOs hidrocarbonetos policíclicos aromáticos (HPAs) são compostos orgânicos semi-voláteis formados principalmente pela combustão incompleta de materiais orgânicos (Grover et al., 2013; Liu et al., 2015), sendo listados como poluentes pela União Européia e pela Agência de Proteção Ambiental dos EUA em solos (Xue et al., 2015). No processo de produção de biocarvão, durante a pirólise, ocorre a formação de HPAs devido aos fragmentos instáveis gerados (Hale et al., 2012). O método tradicional de extração de HPAs é realizado através do Soxhlet (United States, 1996). Em comparação com outros métodos de extração, o Soxhlet apresenta desvantagens como maior tempo despendido, grande quantidade de solvente e como consequência, problemas ambientais (Castro et al., 2010). O método de extração por ultrassom apresenta vantagens como simplicidade, menor tempo de extração e de baixo volume de solventes, diminuindo os possíveis danos ao ambiente (Cardoso et al., 2014). Dessa forma, nosso objetivo foi comparar os métodos de extração, Soxhlet e ultrassom, de acordo com o percentual de recuperação de HPAs em amostras de biocarvão (BC) e mistura de solo/biocarvão (SBC) por cromatografia gasosa.
650 $aAmostragem
650 $aSolo
653 $aAnálise de HPAs
653 $aAnálise de Solo
653 $aBiocarvão
700 1 $aSILVA, B. R. da
700 1 $aMORALES, M. M.
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Registro original: |
Embrapa Florestas (CNPF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/04/2011 |
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Data da última atualização: |
29/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MARTINS, P. K.; MAFRA, V. S.; KISHI, L. T.; FREITAS-ASTUA, J. |
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Afiliação: |
Polyanna Kelly Martins, APTA; Valéria S. Mafra, APTA; Luciano T. Kishi, APTA; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Phylogenetic and comparative gene expression analysis of citrus WRKY transcription factor family. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
pdf 35130 |
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Conteúdo: |
Plants have a variety of active defense mechanisms to protect themselves from pathogen infection. Plant defense response results from the transcriptional activation of a large number of genes upon pathogen infection or treatment with pathogen elicitors. Among them, WRKY proteins are considered important transcriptional regulators in defense signaling, including the activation of SAR (systemic acquired resistance). Several genes regulating plant defense, such as NPR1 and PR (Pathogenesis-related proteins), have W-box elements in their promoters that are specifically recognized by WRKY proteins also required for induction or expression. The defining feature of WRKY transcription factors is their DNA binding domain. The WRKY domain is about 60 residues in length that contains the WRKY signature and also an atypical zinc-finger structure at the C-terminus (Cx4?5Cx22?23HxH or Cx7Cx23HxC). The aim of this work was to identify all potential WRKY transcription factors in citrus, retrieved from the CitEST (Citrus ESTs) database and to construct a phylogenetic tree with orthologs from Arabidopsis thaliana and Oryza sativa. To find ESTs coding for WRKY proteins in citrus we performed a tBLASTn search on the CitEST database. The WRKY domain sequences of seven Arabidopsis WRKY family members each representing one of the WRKY subgroups were used as query sequences. Phylogenetic tree were constructed by the neighbor-joining (NJ) method in MEGA 4. NJ analysis was performed with the Pairwise Deletion option and the Poisson correction. For statistical reliability, bootstrap analysis was conducted with 1,000 replicates to assess statistical support for each node. The primary search resulted in 71 non-redundant hits, of which 9 were removed as they did not contain the conserved WRKY domain signature. The remaining 62 sequences were used to construct the phylogenetic tree; this analysis identified 19 unigenes for the group 1; 6 unigenes for the subgroup 2a, 3 unigenes for the subgroup 2b, 12 unigenes for the subgroup 2c, 13 unigenes for the group 2d, 9 unigenes for subgroup 2e and 7 unigenes for the group 3. To complete the phylogenetic analysis, WRKY sequences from the draft sequencing of the genomes of Citrus clementina and C. sinensis will be included. We are particularly interested in WRKY proteins involved in biotic stress, so the next step will be to evaluate the transcriptional profile of some WRKY genes under different biotic stress conditions. This study will be crucial to select candidates for citrus genetic transformation. MenosPlants have a variety of active defense mechanisms to protect themselves from pathogen infection. Plant defense response results from the transcriptional activation of a large number of genes upon pathogen infection or treatment with pathogen elicitors. Among them, WRKY proteins are considered important transcriptional regulators in defense signaling, including the activation of SAR (systemic acquired resistance). Several genes regulating plant defense, such as NPR1 and PR (Pathogenesis-related proteins), have W-box elements in their promoters that are specifically recognized by WRKY proteins also required for induction or expression. The defining feature of WRKY transcription factors is their DNA binding domain. The WRKY domain is about 60 residues in length that contains the WRKY signature and also an atypical zinc-finger structure at the C-terminus (Cx4?5Cx22?23HxH or Cx7Cx23HxC). The aim of this work was to identify all potential WRKY transcription factors in citrus, retrieved from the CitEST (Citrus ESTs) database and to construct a phylogenetic tree with orthologs from Arabidopsis thaliana and Oryza sativa. To find ESTs coding for WRKY proteins in citrus we performed a tBLASTn search on the CitEST database. The WRKY domain sequences of seven Arabidopsis WRKY family members each representing one of the WRKY subgroups were used as query sequences. Phylogenetic tree were constructed by the neighbor-joining (NJ) method in MEGA 4. NJ analysis was performed with the Pairwise... Mostrar Tudo |
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Palavras-Chave: |
CitEST; SAR; W-box. |
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Thesaurus NAL: |
Citrus; transcription factors. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03255nam a2200217 a 4500
001 1886952
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aMARTINS, P. K.
245 $aPhylogenetic and comparative gene expression analysis of citrus WRKY transcription factor family.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 35130
520 $aPlants have a variety of active defense mechanisms to protect themselves from pathogen infection. Plant defense response results from the transcriptional activation of a large number of genes upon pathogen infection or treatment with pathogen elicitors. Among them, WRKY proteins are considered important transcriptional regulators in defense signaling, including the activation of SAR (systemic acquired resistance). Several genes regulating plant defense, such as NPR1 and PR (Pathogenesis-related proteins), have W-box elements in their promoters that are specifically recognized by WRKY proteins also required for induction or expression. The defining feature of WRKY transcription factors is their DNA binding domain. The WRKY domain is about 60 residues in length that contains the WRKY signature and also an atypical zinc-finger structure at the C-terminus (Cx4?5Cx22?23HxH or Cx7Cx23HxC). The aim of this work was to identify all potential WRKY transcription factors in citrus, retrieved from the CitEST (Citrus ESTs) database and to construct a phylogenetic tree with orthologs from Arabidopsis thaliana and Oryza sativa. To find ESTs coding for WRKY proteins in citrus we performed a tBLASTn search on the CitEST database. The WRKY domain sequences of seven Arabidopsis WRKY family members each representing one of the WRKY subgroups were used as query sequences. Phylogenetic tree were constructed by the neighbor-joining (NJ) method in MEGA 4. NJ analysis was performed with the Pairwise Deletion option and the Poisson correction. For statistical reliability, bootstrap analysis was conducted with 1,000 replicates to assess statistical support for each node. The primary search resulted in 71 non-redundant hits, of which 9 were removed as they did not contain the conserved WRKY domain signature. The remaining 62 sequences were used to construct the phylogenetic tree; this analysis identified 19 unigenes for the group 1; 6 unigenes for the subgroup 2a, 3 unigenes for the subgroup 2b, 12 unigenes for the subgroup 2c, 13 unigenes for the group 2d, 9 unigenes for subgroup 2e and 7 unigenes for the group 3. To complete the phylogenetic analysis, WRKY sequences from the draft sequencing of the genomes of Citrus clementina and C. sinensis will be included. We are particularly interested in WRKY proteins involved in biotic stress, so the next step will be to evaluate the transcriptional profile of some WRKY genes under different biotic stress conditions. This study will be crucial to select candidates for citrus genetic transformation.
650 $aCitrus
650 $atranscription factors
653 $aCitEST
653 $aSAR
653 $aW-box
700 1 $aMAFRA, V. S.
700 1 $aKISHI, L. T.
700 1 $aFREITAS-ASTUA, J.
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