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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
15/09/2021 |
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Data da última atualização: |
15/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENDES, I. V.; GARCIA, M. B.; BITENCOURT, A. C. A.; SANTANA, R. H.; LINS, P. de C.; SILVEIRA, R.; SIMMONS, B. A.; GLADDEN, J. M.; KRUGER, R. H.; QUIRINO, B. F. |
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Afiliação: |
ISIS VIANA MENDES, Universidade de Brasília; MARIANA BOTELHO GARCIA, Universidade Católica de Brasília; ANA CAROLINA ARAUJO BITENCOURT, Universidade de Brasília; RENATA HENRIQUE SANTANA, Instituto Federal de Brasília; PHILIPPE DE CASTRO LINS, Universidade de Brasília; RAFAELLA SILVEIRA, Universidade de Brasília; BLAKE A. SIMMONS, Joint BioEnergy Institute; JOHN M. GLADDEN, Joint BioEnergy Institute; RICARDO HENRIQUE KRUGER, Universidade de Brasília; BETANIA FERRAZ QUIRINO, CNPAE. |
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Título: |
Bacterial diversity dynamics in microbial consortia selected for lignin utilization. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
PLoS ONE, v. 16, n. 9, e0255083, 2021. |
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Descrição Física: |
PDF: il. color. |
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DOI: |
https://doi.org/10.1371/journal.pone.0255083 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lignin used as a carbon source contributed more to differentiate the bacterial consortia than the variable temperature. The main known bacterial genera selected to use lignin as a carbon source were Altererythrobacter, Aminobacter, Bacillus, Burkholderia, Lysinibacillus, Microvirga, Mycobacterium, Ochrobactrum, Paenibacillus, Pseudomonas, Pseudoxanthomonas, Rhizobiales and Sphingobium. These selected bacterial genera can be of particular interest for studying lignin degradation and utilization, as well as for lignin-related biotechnology applications. MenosAbstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lign... Mostrar Tudo |
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Thesagro: |
Biotecnologia; Carbono; Composto Fenólico; Lignina. |
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Thesaurus Nal: |
Application technology; Biotechnology; Carbon; Degradation; Energy conversion; Lignin; Phenolic compounds. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226045/1/Bacterial-diversity-dynamics-in-microbial-2021.pdf
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Marc: |
LEADER 03127naa a2200385 a 4500
001 2134421
005 2021-09-15
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0255083$2DOI
100 1 $aMENDES, I. V.
245 $aBacterial diversity dynamics in microbial consortia selected for lignin utilization.$h[electronic resource]
260 $c2021
300 $cPDF: il. color.
520 $aAbstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lignin used as a carbon source contributed more to differentiate the bacterial consortia than the variable temperature. The main known bacterial genera selected to use lignin as a carbon source were Altererythrobacter, Aminobacter, Bacillus, Burkholderia, Lysinibacillus, Microvirga, Mycobacterium, Ochrobactrum, Paenibacillus, Pseudomonas, Pseudoxanthomonas, Rhizobiales and Sphingobium. These selected bacterial genera can be of particular interest for studying lignin degradation and utilization, as well as for lignin-related biotechnology applications.
650 $aApplication technology
650 $aBiotechnology
650 $aCarbon
650 $aDegradation
650 $aEnergy conversion
650 $aLignin
650 $aPhenolic compounds
650 $aBiotecnologia
650 $aCarbono
650 $aComposto Fenólico
650 $aLignina
700 1 $aGARCIA, M. B.
700 1 $aBITENCOURT, A. C. A.
700 1 $aSANTANA, R. H.
700 1 $aLINS, P. de C.
700 1 $aSILVEIRA, R.
700 1 $aSIMMONS, B. A.
700 1 $aGLADDEN, J. M.
700 1 $aKRUGER, R. H.
700 1 $aQUIRINO, B. F.
773 $tPLoS ONE$gv. 16, n. 9, e0255083, 2021.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| Registros recuperados : 184 | |
| 3. |  | MOLINARI, H. B. C.; QUIRINO, B. F. Biologia avançada em Jatropha. In: WORKSHOP INTERNO DE P&DI EM JATROPHA SPP. PARA PRODUÇÃO DE BIODIESEL, 2008, Brasília, DF. Balanço do esforço corrente e realinhamento de atividades. Brasília, DF: Embrapa Agroenergia, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Agroenergia. |
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| 11. | | SANTANA, B. G.; ALLEN, C.; QUIRINO, B. F. Classificação em filotipo de isolados brasileiros de Ralstonia solanacearum da Biovar 2. Tropical Plant Pathology, Brasília, DF, v. 33, p. S93, ago. 2008. Suplemento. Resumo BAC 019. Trabalho apresentado no 41. Congresso Brasileiro de Fitopatologia, 41. Annual Meeting of the Brazilian Phytopathological Society, Belo Horizonte, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 16. | | TAVARES, P.; SILVA, M. R. S. S.; KRUGER, R. H.; NORONHA, E. F.; QUIRINO, B. F. Accessing lipases from soil metagenoma and their application for microbial biofuel production. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçu, PR. Anais... São Paulo: Sociedade Brasileira de Microbiologia, 2011. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | TAVARES, P.; BERGMANN, J.; KRUGER, R. H.; NORONHA, E.; QUIRINO, B. F. Accessing lipases from soil metagenome as an aid for biofuel production. In: BRAZILIAN BIOENERGY SCIENCE AND TECHNOLOGY CONFERENCE - BBEST, 1., 2011, Campos do Jordão, SP. [Anais...]. São Paulo: FAPESP, 2011. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 18. | | BALESTRINI, V. P.; PINTO, O. H. B.; KRÜGER, R. H.; QUIRINO, B. F. Análise de genomas montados a partir de uma comunidade microbiana enriquecida com lignina. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 7., 2023, Brasília, DF. Anais... Brasília, DF: Embrapa, 2023. p. 10.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 19. | | MURAD, A. M.; MOLINARI, H. B. C.; TAKAHASHI, F.; FRANCO, O. L.; QUIRINO, B. F. Análises proteômicas e fisiológicas em Saccharum spp. submetidas a estresse salino. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 105.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 184 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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