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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
03/11/2010 |
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Data da última atualização: |
28/03/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SALES, H. R.; SILVA, R. A.; QUINTELA, E. D.; POSSEBOM, A.; BARRIGOSSI, J. A. F. |
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Afiliação: |
HELLEN ROSA SALES, bolsista CNPAF; RODRIGO ALVES DA SILVA, CNPAF; ELIANE DIAS QUINTELA, CNPAF; ADRIANO DAVI POSSEBOM, bolsista CNPAF; JOSE ALEXANDRE F BARRIGOSSI, CNPAF. |
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Título: |
Aumento da patogenicidade de Metarhizium anisopliae ao Tibraca limbativentris com dose subletal de Tiametoxan. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: SEMINÁRIO JOVENS TALENTOS, 4., 2010, Santo Antônio de Goiás. Resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2010. |
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Páginas: |
p. 21. |
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Série: |
(Embrapa Arroz e Feijão. Documentos, 257). |
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Idioma: |
Português |
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Conteúdo: |
O percevejo-do-colmo, Tibraca limbativentris tem apresentado alta incidência em lavouras de arroz, provocando perdas na produção de 5% a 80%. Determinou-se o efeito de dose subletal de Tiametoxan em combinação com M. anisopliae no controle do T. limbativentris em nível de campo. |
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Palavras-Chave: |
Tiametoxan. |
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Thesagro: |
Fungo; Metarhizium anisopliae; Percevejo; Tibraca limbativentris. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31245/1/p21.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/23407/1/cbe8.pdf
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Marc: |
LEADER 01128nam a2200241 a 4500
001 1883194
005 2011-03-28
008 2010 bl uuuu u00u1 u #d
100 1 $aSALES, H. R.
245 $aAumento da patogenicidade de Metarhizium anisopliae ao Tibraca limbativentris com dose subletal de Tiametoxan.$h[electronic resource]
260 $aIn: SEMINÁRIO JOVENS TALENTOS, 4., 2010, Santo Antônio de Goiás. Resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão$c2010
300 $ap. 21.
490 $a(Embrapa Arroz e Feijão. Documentos, 257).
520 $aO percevejo-do-colmo, Tibraca limbativentris tem apresentado alta incidência em lavouras de arroz, provocando perdas na produção de 5% a 80%. Determinou-se o efeito de dose subletal de Tiametoxan em combinação com M. anisopliae no controle do T. limbativentris em nível de campo.
650 $aFungo
650 $aMetarhizium anisopliae
650 $aPercevejo
650 $aTibraca limbativentris
653 $aTiametoxan
700 1 $aSILVA, R. A.
700 1 $aQUINTELA, E. D.
700 1 $aPOSSEBOM, A.
700 1 $aBARRIGOSSI, J. A. F.
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Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
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Origem |
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Classificação |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
19/01/2009 |
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Data da última atualização: |
24/07/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KOBAYASHI, A. K.; DIAZ TRUJILLO, C.; LEE, T. van der; ZWIERS, L.-H.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. |
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Afiliação: |
ADILSON KENJI KOBAYASHI, CPAMN; CAUCASELLA DIAZ TRUJILLO, PLANT RESEARCH INTERNATIONAL; THEO VAN DER LEE; LUTE-HARM ZWIERS; LUCIANO V. PAIVA, FEDERAL UNIVERSITY OF LAVRAS; MANOEL TEIXEIRA SOUZA JUNIOR, EMBRAPA LABEX EUROPE; GERT H. J. KEMA, PLANT RESEARCH INTERNATIONAL. |
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Título: |
Generation of transgenic Mycosphaerella fijiensis expressing reporter genes: tools for pathogenicity and mating studies. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Mycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana. MenosMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducte... Mostrar Tudo |
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Palavras-Chave: |
CIRAD139A; CIRAD86; DsRed; GFP. |
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Thesagro: |
Doença; Fungo. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/69938/1/GenerationTransgenic-22676.pdf
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Marc: |
LEADER 02408nam a2200253 a 4500
001 1069938
005 2023-07-24
008 2008 bl uuuu u00u1 u #d
100 1 $aKOBAYASHI, A. K.
245 $aGeneration of transgenic Mycosphaerella fijiensis expressing reporter genes$btools for pathogenicity and mating studies.$h[electronic resource]
260 $aIn: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland$c2008
520 $aMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana.
650 $aDoença
650 $aFungo
653 $aCIRAD139A
653 $aCIRAD86
653 $aDsRed
653 $aGFP
700 1 $aDIAZ TRUJILLO, C.
700 1 $aLEE, T. van der
700 1 $aZWIERS, L.-H.
700 1 $aPAIVA, L. V.
700 1 $aSOUZA JUNIOR, M. T.
700 1 $aKEMA, G. H. J.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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