Data/hora: 05/06/2024 13:11:09

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 14/10/2020
Data da última atualização: | 30/11/2020
Autoria: | TERRA. L. R.; FERREIRA, M. M. C.; TERAO, D.; QUEIROZ, S. C. do N. de.
Afiliação: | LARISSA ROCHA TERRA, IQ-UNICAMP; MARCIA MIGUEL CASTRO FERREIRA, IQ-UNICAMP; DANIEL TERAO, CNPMA; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA.
Título: | Otimização do processo não invasivo e não destrutivo de extração e análise de metabólitos voláteis de mamão papaia por SPME-GC-MS.
Ano de publicação: | 2020
Fonte/Imprenta: | Química Nova, v. 43, n. 9, p. 1240-1245, 2020.
ISSN: | 1678-7064
DOI: | http://dx.doi.org/10.21577/0100-4042.20170611
Idioma: | Português
Conteúdo: | Abstract: The analysis of volatiles is an alternative in the routine of quality control of fruits, since the volatile metabolites can change in the presence of microorganisms. A well-established method for extracting volatiles is the solid phase microextraction (SPME). This work aims to define some parameters (fiber coating, conditioning time and fiber exposure time) for an effective extraction. Whole papaya was inserted into a hermetically closed glass bottle and the system was left in conditioning for the volatiles accumulation. Then, extraction by SPME and subsequent desorption in a gas chromatograph was performed. Two fibers were evaluated: a 100 &#956;m polydimethylsiloxane (PDMS) and a 65 &#956;m polydimethylsiloxane/divinylbenzene (PDMS/DVB). The PDMS/DVB fiber extracted the highest number of volatiles. Central composite design (CCD) was performed to assess the effect of papaya conditioning time and the fiber exposure time on the response: number of detectable peaks. Both variables studied and their interactions were significant for the response. ANOVA showed that the quadratic terms and the lack of fit were not significant (p <0.05). A conditioning time of 10 min and exposure time of 30 min was sufficient for the detection of more than 100 compounds.
Palavras-Chave: | Central composite design; Fruit; Response surface methodology Metabolomics.
Thesagro: | Análise Química; Carica Papaya; Cromatografia Gasosa; Espectrometria; Mamão; Microrganismo.
Thesaurus Nal: | Chemical analysis; Gas chromatography; Mass spectrometry; Metabolites; Microbial detection; Papayas; Solid phase microextraction; Volatile organic compounds.

Categoria do assunto:

H Saúde e Patologia
W Química e Física

URL:

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/218363/1/Terao-Optimization-extraction-2020.pdf
http://ainfo.cnptia.embrapa.br/digital/bitstream/item/216646/1/Terao-Otimizacao-processo-2020.pdf

Registro original: | Embrapa Meio Ambiente (CNPMA)

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