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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
05/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TEIXEIRA, J. A.; RIBEIRO, J. B.; GONÇALVES, D. B.; QUEIROZ, M. V. de; ARAÚJO, E. F. de. |
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Afiliação: |
JANAINA APARECIDA TEIXEIRA, UFV; JOAO BATISTA RIBEIRO, CNPGL; DANIEL BONOTO GONÇALVES, UFSJ; MARISA VIEIRA DE QUEIROZ, UFV; ELZA FERNANDES DE ARAÚJO, UFV. |
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Título: |
Overproduction of polygalacturonase by Penicillium griseoroseum recombinant strains and functional analysis by targeted disruption of the pgg2 gene. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Applied Biochemistry and Biotechnology, v. 169, n. 6, p. 1965-1977, 2013. |
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DOI: |
https://doi.org/10.1007/s12010-013-0121-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Inactivation of the pgg2 gene, a polygalacturonase-encoding gene from Penicillium griseoroseum, reduced the total activity of polygalacturonase (PG) by 90 % in wild-type P. griseoroseum, which indicates that the pgg2 gene is the major gene responsible for PG production in this species. To increase PG production, the coding region of the pgg2 gene was cloned under the control of the glyceraldehyde 3-phosphate dehydrogenase (gpd) promoter and the terminator region of the tryptophan synthase (trpC) gene from Aspergillus nidulans (pAN52pgg2 vector). This vector was then used to transform P. griseoroseum. The transformed strains were characterized according to PG production using glucose, sucrose, or sugar cane juice as the carbon sources. The recombinant P. griseoroseum T146 strain contained an additional copy of the pgg2 gene, which resulted in a 12-fold increase in PG activity when compared with that detected in the supernatant of the control PG63 strain. The proteins secreted by the recombinant strain T146 showed a strong band at 38 kDa, which corresponds to the molecular weight of PG of the P. griseoroseum. The results demonstrate the significant biotechnological potential of recombinant P. griseoroseum T146 for use in PG production. |
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Palavras-Chave: |
Gene inactivation; Gpd promoter; Overproduction; Penicillium griseoroseum. |
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Thesaurus Nal: |
polygalacturonase. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 02088naa a2200241 a 4500
001 1978792
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s12010-013-0121-6$2DOI
100 1 $aTEIXEIRA, J. A.
245 $aOverproduction of polygalacturonase by Penicillium griseoroseum recombinant strains and functional analysis by targeted disruption of the pgg2 gene.$h[electronic resource]
260 $c2013
520 $aInactivation of the pgg2 gene, a polygalacturonase-encoding gene from Penicillium griseoroseum, reduced the total activity of polygalacturonase (PG) by 90 % in wild-type P. griseoroseum, which indicates that the pgg2 gene is the major gene responsible for PG production in this species. To increase PG production, the coding region of the pgg2 gene was cloned under the control of the glyceraldehyde 3-phosphate dehydrogenase (gpd) promoter and the terminator region of the tryptophan synthase (trpC) gene from Aspergillus nidulans (pAN52pgg2 vector). This vector was then used to transform P. griseoroseum. The transformed strains were characterized according to PG production using glucose, sucrose, or sugar cane juice as the carbon sources. The recombinant P. griseoroseum T146 strain contained an additional copy of the pgg2 gene, which resulted in a 12-fold increase in PG activity when compared with that detected in the supernatant of the control PG63 strain. The proteins secreted by the recombinant strain T146 showed a strong band at 38 kDa, which corresponds to the molecular weight of PG of the P. griseoroseum. The results demonstrate the significant biotechnological potential of recombinant P. griseoroseum T146 for use in PG production.
650 $apolygalacturonase
653 $aGene inactivation
653 $aGpd promoter
653 $aOverproduction
653 $aPenicillium griseoroseum
700 1 $aRIBEIRO, J. B.
700 1 $aGONÇALVES, D. B.
700 1 $aQUEIROZ, M. V. de
700 1 $aARAÚJO, E. F. de
773 $tApplied Biochemistry and Biotechnology$gv. 169, n. 6, p. 1965-1977, 2013.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Hortaliças. |
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Data corrente: |
09/06/2020 |
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Data da última atualização: |
09/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CARVALHO, S. I. C. de; RAGASSI, C. F.; FALEIRO, F. G.; BIANCHETTI, L. de B.; LIMA, M. F.; REIFSCHNEIDER, F. J. B.; RIBEIRO, C. S. da C. |
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Afiliação: |
SABRINA ISABEL COSTA DE CARVALHO, CNPH; CARLOS FRANCISCO RAGASSI, CNPH; FABIO GELAPE FALEIRO, CPAC; LUCIANO DE BEM BIANCHETTI, Cenargen; MIRTES FREITAS LIMA, CNPH; FRANCISCO JOSE BECKER REIFSCHNEIDER, SIRE; CLAUDIA SILVA DA COSTA RIBEIRO, CNPH. |
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Título: |
Establishment of Capsicum frutescenscore collections based on morphological and molecular descriptors and on virus incidence. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 19, n. 1, gmr18503, 2020. |
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Páginas: |
19 p. |
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DOI: |
http://dx.doi.org/10.4238/gmr18503 |
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Idioma: |
Português |
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Conteúdo: |
A germplasm collection of C. frutescensis maintained at Embrapa Vegetables, Brasilia, Brazil, the branch for vegetable crops of the Brazilian Agricultural Research Corporation (EMBRAPA). This is considered to be the main collection representing the variability of this species in the country. |
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Thesagro: |
Banco de Germoplasma; Pimenta Malagueta. |
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Thesaurus NAL: |
Capsicum frutescens; Pepper. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/213875/1/gmr18503-establishment-capsicum-frutescens-core-collections-based-morphological-and-molecular-0.pdf
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Marc: |
LEADER 01145naa a2200265 a 4500
001 2123180
005 2020-06-09
008 2020 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/gmr18503$2DOI
100 1 $aCARVALHO, S. I. C. de
245 $aEstablishment of Capsicum frutescenscore collections based on morphological and molecular descriptors and on virus incidence.$h[electronic resource]
260 $c2020
300 $a19 p.
520 $aA germplasm collection of C. frutescensis maintained at Embrapa Vegetables, Brasilia, Brazil, the branch for vegetable crops of the Brazilian Agricultural Research Corporation (EMBRAPA). This is considered to be the main collection representing the variability of this species in the country.
650 $aCapsicum frutescens
650 $aPepper
650 $aBanco de Germoplasma
650 $aPimenta Malagueta
700 1 $aRAGASSI, C. F.
700 1 $aFALEIRO, F. G.
700 1 $aBIANCHETTI, L. de B.
700 1 $aLIMA, M. F.
700 1 $aREIFSCHNEIDER, F. J. B.
700 1 $aRIBEIRO, C. S. da C.
773 $tGenetics and Molecular Research$gv. 19, n. 1, gmr18503, 2020.
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Registro original: |
Embrapa Hortaliças (CNPH) |
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ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
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