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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
27/07/2022 |
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Data da última atualização: |
10/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PELLIN, G. P.; MARTINS, R. A.; QUEIROZ, C. A. de; SOUSA, T. F.; MUNIZ, A. W.; SILVA, G. F. da; MAJOLO, C. |
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Afiliação: |
GIULIENE PEREIRA PELLIN, Programa Institucional de Apoio à Iniciação Científica do Amazonas (PAIC), CPAA; RAESLEN ARAÚJO MARTINS, Programa Institucional de Apoio à Iniciação Científica do Amazonas (PAIC), CPAA; CLAUDIA AFRAS DE QUEIROZ, Programa de Pós-graduação em Biotecnologia, UFAM; THIAGO FERNANDES SOUSA, Programa de Pós-graduação em Biotecnologia, UFAM; ALEKSANDER WESTPHAL MUNIZ, CPAA; GILVAN FERREIRA DA SILVA, CPAA; CLAUDIA MAJOLO, CPAA. |
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Título: |
Aeromonas from farmed tambaqui from North Brazil: molecular identification and pathogenic potential. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Ciência Rural, v. 53, n. 4, art. e20220151, 2023. |
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DOI: |
http://doi.org/10.1590/0103-8478cr20220151 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Aeromonas de tambaqui de cultivo do norte do Brasil: identificação molecular e potencial patogênico. |
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Conteúdo: |
ABSTRACT: The aim of this study was to molecularly identify different species of Aeromonas isolated from farmed tambaqui (Colossoma macropomum) from North Brazil, and evaluate their pathogenic potential by the presence of virulence genes. From the extraction of bacterial DNA, PCR (polymerase chain reaction) of the primers 16S rDNA, aerA (cytolytic enterotoxin), ast (cytotoxic enterotoxin) and act (cytotoxic enterotoxin) were performed. Of 24 isolates evaluated, eight amplified the ast gene, one amplified the act gene, but the areA gene was not amplified in any isolate. Sequencing of the 16S rRNA primer revealed a predominance of Aeromonas jandaei specie (92%). Aeromonas taiwanensis (4%), for the first time isolated from fish in Brazil, and Aeromonas hydrophila (4%) each appeared as just one isolate. Results showed that 32% of Aeromonas isolated from farmed tambaqui have considerable pathogenic potential for systemic damage, since the selected PCR primers are encoding the most common virulence genes in Aeromonas with high pathogenic intensity. RESUMO: O objetivo deste estudo foi identificar molecularmente diferentes espécies de Aeromonas isoladas de tambaqui (Colossoma macropomum) de cultivo do norte do Brasil e avaliar seu potencial patogênico pela presença de genes de virulência. A partir da extração do DNA bacteriano, foi realizada a PCR (reação em cadeia da polimerase) dos primers 16S rDNA, aerA (enterotoxina citolítica), ast (enterotoxina citotóxica) e act (enterotoxina citotóxica). Dos 24 isolados avaliados, oito amplificaram o gene ast, um amplificou o gene act, mas o gene areA não foi amplificado em nenhum isolado. O sequenciamento do primer 16S rRNA revelou uma predominância da espécie Aeromonas jandaei (92%). Aeromonas taiwanensis (4%), pela primeira vez isolada em peixes no Brasil, e Aeromonas hydrophila (4%) apareceram cada uma como apenas um isolado. Os resultados mostram que 32% das Aeromonas isoladas de tambaqui de cultivo apresentam considerável potencial patogênico para danos sistêmicos, uma vez que os primers de PCR selecionados estão codificando os genes de virulência mais comuns em Aeromonas com alta intensidade patogênica. MenosABSTRACT: The aim of this study was to molecularly identify different species of Aeromonas isolated from farmed tambaqui (Colossoma macropomum) from North Brazil, and evaluate their pathogenic potential by the presence of virulence genes. From the extraction of bacterial DNA, PCR (polymerase chain reaction) of the primers 16S rDNA, aerA (cytolytic enterotoxin), ast (cytotoxic enterotoxin) and act (cytotoxic enterotoxin) were performed. Of 24 isolates evaluated, eight amplified the ast gene, one amplified the act gene, but the areA gene was not amplified in any isolate. Sequencing of the 16S rRNA primer revealed a predominance of Aeromonas jandaei specie (92%). Aeromonas taiwanensis (4%), for the first time isolated from fish in Brazil, and Aeromonas hydrophila (4%) each appeared as just one isolate. Results showed that 32% of Aeromonas isolated from farmed tambaqui have considerable pathogenic potential for systemic damage, since the selected PCR primers are encoding the most common virulence genes in Aeromonas with high pathogenic intensity. RESUMO: O objetivo deste estudo foi identificar molecularmente diferentes espécies de Aeromonas isoladas de tambaqui (Colossoma macropomum) de cultivo do norte do Brasil e avaliar seu potencial patogênico pela presença de genes de virulência. A partir da extração do DNA bacteriano, foi realizada a PCR (reação em cadeia da polimerase) dos primers 16S rDNA, aerA (enterotoxina citolítica), ast (enterotoxina citotóxica) e act (enterotoxina ... Mostrar Tudo |
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Palavras-Chave: |
Aeromonas taiwanensis; Genes de virulência; Reação da polimerase em cadeia; Virulence genes. |
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Thesagro: |
Colossoma Macropomum; Tambaqui. |
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Thesaurus Nal: |
Polymerase chain reaction. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144999/1/Aeromonas-from-farmed.pdf
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Marc: |
LEADER 03246naa a2200301 a 4500
001 2144999
005 2023-02-10
008 2023 bl uuuu u00u1 u #d
024 7 $ahttp://doi.org/10.1590/0103-8478cr20220151$2DOI
100 1 $aPELLIN, G. P.
245 $aAeromonas from farmed tambaqui from North Brazil$bmolecular identification and pathogenic potential.$h[electronic resource]
260 $c2023
500 $aTítulo em português: Aeromonas de tambaqui de cultivo do norte do Brasil: identificação molecular e potencial patogênico.
520 $aABSTRACT: The aim of this study was to molecularly identify different species of Aeromonas isolated from farmed tambaqui (Colossoma macropomum) from North Brazil, and evaluate their pathogenic potential by the presence of virulence genes. From the extraction of bacterial DNA, PCR (polymerase chain reaction) of the primers 16S rDNA, aerA (cytolytic enterotoxin), ast (cytotoxic enterotoxin) and act (cytotoxic enterotoxin) were performed. Of 24 isolates evaluated, eight amplified the ast gene, one amplified the act gene, but the areA gene was not amplified in any isolate. Sequencing of the 16S rRNA primer revealed a predominance of Aeromonas jandaei specie (92%). Aeromonas taiwanensis (4%), for the first time isolated from fish in Brazil, and Aeromonas hydrophila (4%) each appeared as just one isolate. Results showed that 32% of Aeromonas isolated from farmed tambaqui have considerable pathogenic potential for systemic damage, since the selected PCR primers are encoding the most common virulence genes in Aeromonas with high pathogenic intensity. RESUMO: O objetivo deste estudo foi identificar molecularmente diferentes espécies de Aeromonas isoladas de tambaqui (Colossoma macropomum) de cultivo do norte do Brasil e avaliar seu potencial patogênico pela presença de genes de virulência. A partir da extração do DNA bacteriano, foi realizada a PCR (reação em cadeia da polimerase) dos primers 16S rDNA, aerA (enterotoxina citolítica), ast (enterotoxina citotóxica) e act (enterotoxina citotóxica). Dos 24 isolados avaliados, oito amplificaram o gene ast, um amplificou o gene act, mas o gene areA não foi amplificado em nenhum isolado. O sequenciamento do primer 16S rRNA revelou uma predominância da espécie Aeromonas jandaei (92%). Aeromonas taiwanensis (4%), pela primeira vez isolada em peixes no Brasil, e Aeromonas hydrophila (4%) apareceram cada uma como apenas um isolado. Os resultados mostram que 32% das Aeromonas isoladas de tambaqui de cultivo apresentam considerável potencial patogênico para danos sistêmicos, uma vez que os primers de PCR selecionados estão codificando os genes de virulência mais comuns em Aeromonas com alta intensidade patogênica.
650 $aPolymerase chain reaction
650 $aColossoma Macropomum
650 $aTambaqui
653 $aAeromonas taiwanensis
653 $aGenes de virulência
653 $aReação da polimerase em cadeia
653 $aVirulence genes
700 1 $aMARTINS, R. A.
700 1 $aQUEIROZ, C. A. de
700 1 $aSOUSA, T. F.
700 1 $aMUNIZ, A. W.
700 1 $aSILVA, G. F. da
700 1 $aMAJOLO, C.
773 $tCiência Rural$gv. 53, n. 4, art. e20220151, 2023.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
21/02/2017 |
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Data da última atualização: |
06/03/2017 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SOLÓRZANO, L. A. R.; MARINO, C. T.; RODRIGUES, P. H. M.; BERNDT, A.; PINEDO, L. A.; FRIGHETTO, R. T. S.; OLIVEIRA, P. P. A.; PRAES, M. F. F. M. |
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Afiliação: |
LAURA ALEXANDRA ROMERO SOLÓRZANO, FMVZ-USP; CAROLINA TOBIAS MARINO, FMVZ-USP; PAULO HENRIQUE MAZZA RODRIGUES, FMZV-USP; ALEXANDRE BERNDT, CPPSE; LERNER AREVALO PINEDO, FMVZ-USP; ROSA TOYOKO SHIRAISHI FRIGHETTO, CNPMA; PATRICIA PERONDI ANCHAO OLIVEIRA, CPPSE; MARIA FERNANDA FERREIRA MENEGUCCI PRAES. |
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Título: |
Effect of energy source inclusion in diet on methane production of cattle determined by sulphur hexafluoride (SF6) tracer gas technique. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON GREENHOUSE GASES IN AGRICULTURE, 2., 2016, Campo Grande, MS. Proceedings... Brasília, DF: Embrapa, 2016. p. 235-240. (Embrapa Gado de Corte. Documentos, 216). |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Ruminant animals have a great advantage over simple-stomached animals, as their digestive process is able to release the energy contained in cellulosic material through carbohydrate fermentation by microorganisms enzymes from the rumen environment. However, carbohydrate fermentation results not only in short chain fatty acids (SCFA) but also in less desirable products such as heat, as well as methane (CH4) and carbon dioxide (CO2) gases which represents energy loss for the animal estimated in 2 to 12% of gross energy from feed (Johnson and Johnson, 1995). As an adult ruminant can produce up to 17 liters of methane per hour and this gas cannot be metabolized even by rumen microorganisms, most of it is removed from rumen by expiration or eructation (Moss, 1993), and released in the environment. There are different techniques for methane emissions measurements by ruminants in production conditions; among them there is one that uses an inert gas tracer, sulphur hexafluoride (SF6). This technique results in a precise estimative of methane production by the animal besides enabling the evaluation of animals in normal pasture conditions, it consists in placing a permeation tube, which releases SF6 at a previously known rate in the rumen, where by the contractions of this organ, CH4 and SF6 gases are released by eructation and samples are collected close to mouth and nostrils. This method assumes that the standard of SF6 emission simulates the standard of CH4 emission. The flow of CH4 released by the animal is calculated in relation to SF6 flow (Westberg et al., 1998).The objective with the present study was to evaluate energy sources inclusion in cattle diets on methane production determined by sulphur hexafluoride (SF6) tracer gas technique. MenosAbstract: Ruminant animals have a great advantage over simple-stomached animals, as their digestive process is able to release the energy contained in cellulosic material through carbohydrate fermentation by microorganisms enzymes from the rumen environment. However, carbohydrate fermentation results not only in short chain fatty acids (SCFA) but also in less desirable products such as heat, as well as methane (CH4) and carbon dioxide (CO2) gases which represents energy loss for the animal estimated in 2 to 12% of gross energy from feed (Johnson and Johnson, 1995). As an adult ruminant can produce up to 17 liters of methane per hour and this gas cannot be metabolized even by rumen microorganisms, most of it is removed from rumen by expiration or eructation (Moss, 1993), and released in the environment. There are different techniques for methane emissions measurements by ruminants in production conditions; among them there is one that uses an inert gas tracer, sulphur hexafluoride (SF6). This technique results in a precise estimative of methane production by the animal besides enabling the evaluation of animals in normal pasture conditions, it consists in placing a permeation tube, which releases SF6 at a previously known rate in the rumen, where by the contractions of this organ, CH4 and SF6 gases are released by eructation and samples are collected close to mouth and nostrils. This method assumes that the standard of SF6 emission simulates the standard of CH4 emission. The ... Mostrar Tudo |
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Thesagro: |
Bovino; Efeito estufa; Metano; Nutrição animal. |
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Thesaurus NAL: |
Cattle; Greenhouse gas emissions; Methane; Sulfur hexafluoride; Tracer techniques. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02851naa a2200313 a 4500
001 2064973
005 2017-03-06
008 2016 bl --- 0-- u #d
100 1 $aSOLÓRZANO, L. A. R.
245 $aEffect of energy source inclusion in diet on methane production of cattle determined by sulphur hexafluoride (SF6) tracer gas technique.$h[electronic resource]
260 $c2016
520 $aAbstract: Ruminant animals have a great advantage over simple-stomached animals, as their digestive process is able to release the energy contained in cellulosic material through carbohydrate fermentation by microorganisms enzymes from the rumen environment. However, carbohydrate fermentation results not only in short chain fatty acids (SCFA) but also in less desirable products such as heat, as well as methane (CH4) and carbon dioxide (CO2) gases which represents energy loss for the animal estimated in 2 to 12% of gross energy from feed (Johnson and Johnson, 1995). As an adult ruminant can produce up to 17 liters of methane per hour and this gas cannot be metabolized even by rumen microorganisms, most of it is removed from rumen by expiration or eructation (Moss, 1993), and released in the environment. There are different techniques for methane emissions measurements by ruminants in production conditions; among them there is one that uses an inert gas tracer, sulphur hexafluoride (SF6). This technique results in a precise estimative of methane production by the animal besides enabling the evaluation of animals in normal pasture conditions, it consists in placing a permeation tube, which releases SF6 at a previously known rate in the rumen, where by the contractions of this organ, CH4 and SF6 gases are released by eructation and samples are collected close to mouth and nostrils. This method assumes that the standard of SF6 emission simulates the standard of CH4 emission. The flow of CH4 released by the animal is calculated in relation to SF6 flow (Westberg et al., 1998).The objective with the present study was to evaluate energy sources inclusion in cattle diets on methane production determined by sulphur hexafluoride (SF6) tracer gas technique.
650 $aCattle
650 $aGreenhouse gas emissions
650 $aMethane
650 $aSulfur hexafluoride
650 $aTracer techniques
650 $aBovino
650 $aEfeito estufa
650 $aMetano
650 $aNutrição animal
700 1 $aMARINO, C. T.
700 1 $aRODRIGUES, P. H. M.
700 1 $aBERNDT, A.
700 1 $aPINEDO, L. A.
700 1 $aFRIGHETTO, R. T. S.
700 1 $aOLIVEIRA, P. P. A.
700 1 $aPRAES, M. F. F. M.
773 $tIn: INTERNATIONAL SYMPOSIUM ON GREENHOUSE GASES IN AGRICULTURE, 2., 2016, Campo Grande, MS. Proceedings... Brasília, DF: Embrapa, 2016. p. 235-240. (Embrapa Gado de Corte. Documentos, 216).
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