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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
05/01/2016 |
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Data da última atualização: |
17/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BOSCO, D. D.; SINSKI, I.; COMACHIO, V.; MAIA, J. D. G.; RITSCHEL, P. S.; QUECINI, V. |
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Afiliação: |
DANIELA DAL BOSCO, CNPUV; IRACI SINSKI, CNPUV; VALTAIR COMACHIO, CNPUV; JOAO DIMAS GARCIA MAIA, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; VERA MARIA QUECINI, CNPUV. |
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Título: |
In Vitro techniques for grapevine germplasm conservation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Acta Horticultura, n. 1082, Apr. 2015. p. 201-206. |
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Idioma: |
Português |
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Conteúdo: |
Abstract Plant genetic resources hold significant phenotypic variation resultant from the presence of allelic diversity, which is maintained by evolutionary processes or by artificial selection. Therefore, plant germplasm encompasses the huge genotypic diversity found in wild and cultivated species and constitutes a source of interesting traits for breeders. Although extremely valuable, biodiversity conservation has high demand for funding, physical space and labor. In vitro conservation is an interesting alternative for the maintenance of highly-heterozygous, vegetatively propagated, perennial species, such as grapevine. It also contributes to the plants? phytosanitary conditions. The current work aimed to develop effective and feasible means toward in vitro establishment and conservation of grapevine germplasm. Woody stakes were obtained from the field collection of the Grapevine Germplasm Bank, at Embrapa, and were surface disinfected, planted in a mixture of autoclaved soil and vermiculite (1:1), and kept under controlled temperature (23±5°C) and relative humidity (70%). Young apical shoots were excised and superficially disinfected in the presence of 1% (w/v) polyvinylpirrolidone. Explants were transferred to tubes containing Galzy medium with active charcoal, under aseptic conditions. Established plants were propagated and maintained in vitro as duplicates. For long-term conservation, the effectiveness of two cryopreservation techniques; vitrification and encapsulationdehydration, was compared for 11 grapevine genotypes, including Vitis vinifera, V. labrusca and hybrids V. berlandieri × V. rupestris, and V. riparia × V. berlandieri. Shoot induction from treated stakes under protected greenhouse conditions significantly reduced environmental contamination and, along with the use of antioxidant agents, allowed in vitro establishment of approximately 1200 (85% of the accessions held by the bank) grapevine accessions. The establishment of the remaining accessions is underway. Plants free of ectophytes were produced for 900 (64.3%) accessions. Cryogenic protocols require further adjustments to allow acceptable recovery rates. High-scale in vitro conservation of grapevine germplasm is feasible and may safeguard valuable biodiversity. Although promising, cryopreservation requires further studies for protocol optimization. MenosAbstract Plant genetic resources hold significant phenotypic variation resultant from the presence of allelic diversity, which is maintained by evolutionary processes or by artificial selection. Therefore, plant germplasm encompasses the huge genotypic diversity found in wild and cultivated species and constitutes a source of interesting traits for breeders. Although extremely valuable, biodiversity conservation has high demand for funding, physical space and labor. In vitro conservation is an interesting alternative for the maintenance of highly-heterozygous, vegetatively propagated, perennial species, such as grapevine. It also contributes to the plants? phytosanitary conditions. The current work aimed to develop effective and feasible means toward in vitro establishment and conservation of grapevine germplasm. Woody stakes were obtained from the field collection of the Grapevine Germplasm Bank, at Embrapa, and were surface disinfected, planted in a mixture of autoclaved soil and vermiculite (1:1), and kept under controlled temperature (23±5°C) and relative humidity (70%). Young apical shoots were excised and superficially disinfected in the presence of 1% (w/v) polyvinylpirrolidone. Explants were transferred to tubes containing Galzy medium with active charcoal, under aseptic conditions. Established plants were propagated and maintained in vitro as duplicates. For long-term conservation, the effectiveness of two cryopreservation techniques; vitrification and encapsulation... Mostrar Tudo |
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Palavras-Chave: |
In vitro; Phytosanitary improvement. |
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Thesaurus Nal: |
Germplasm; Micropropagation; Tissue culture. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136629/1/Ritschel-Actahort-n1082-Apr2015.pdf
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Marc: |
LEADER 03023naa a2200241 a 4500
001 2032995
005 2016-03-17
008 2015 bl uuuu u00u1 u #d
100 1 $aBOSCO, D. D.
245 $aIn Vitro techniques for grapevine germplasm conservation.$h[electronic resource]
260 $c2015
520 $aAbstract Plant genetic resources hold significant phenotypic variation resultant from the presence of allelic diversity, which is maintained by evolutionary processes or by artificial selection. Therefore, plant germplasm encompasses the huge genotypic diversity found in wild and cultivated species and constitutes a source of interesting traits for breeders. Although extremely valuable, biodiversity conservation has high demand for funding, physical space and labor. In vitro conservation is an interesting alternative for the maintenance of highly-heterozygous, vegetatively propagated, perennial species, such as grapevine. It also contributes to the plants? phytosanitary conditions. The current work aimed to develop effective and feasible means toward in vitro establishment and conservation of grapevine germplasm. Woody stakes were obtained from the field collection of the Grapevine Germplasm Bank, at Embrapa, and were surface disinfected, planted in a mixture of autoclaved soil and vermiculite (1:1), and kept under controlled temperature (23±5°C) and relative humidity (70%). Young apical shoots were excised and superficially disinfected in the presence of 1% (w/v) polyvinylpirrolidone. Explants were transferred to tubes containing Galzy medium with active charcoal, under aseptic conditions. Established plants were propagated and maintained in vitro as duplicates. For long-term conservation, the effectiveness of two cryopreservation techniques; vitrification and encapsulationdehydration, was compared for 11 grapevine genotypes, including Vitis vinifera, V. labrusca and hybrids V. berlandieri × V. rupestris, and V. riparia × V. berlandieri. Shoot induction from treated stakes under protected greenhouse conditions significantly reduced environmental contamination and, along with the use of antioxidant agents, allowed in vitro establishment of approximately 1200 (85% of the accessions held by the bank) grapevine accessions. The establishment of the remaining accessions is underway. Plants free of ectophytes were produced for 900 (64.3%) accessions. Cryogenic protocols require further adjustments to allow acceptable recovery rates. High-scale in vitro conservation of grapevine germplasm is feasible and may safeguard valuable biodiversity. Although promising, cryopreservation requires further studies for protocol optimization.
650 $aGermplasm
650 $aMicropropagation
650 $aTissue culture
653 $aIn vitro
653 $aPhytosanitary improvement
700 1 $aSINSKI, I.
700 1 $aCOMACHIO, V.
700 1 $aMAIA, J. D. G.
700 1 $aRITSCHEL, P. S.
700 1 $aQUECINI, V.
773 $tActa Horticultura$gn. 1082, Apr. 2015. p. 201-206.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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| Registros recuperados : 95 | |
| 2. |  | RITSCHEL, P. S.; QUECINI, V. Suco de Uva. In: REIFSCHNEIDER, F. J. B.; NASS, L. L.; HEINRICH, A. G.; RIBEIRO, C. S. da C.; HENZ, G. P.; EUCLIDES FILHO, K.; BOITEUX, L. S.; RITSCHEL, P. S.; FERRAZ, R. M.; QUECINI, V. Uma pitada de biodiversidade na mesa dos brasileiros. Brasília, DF: [s.n.], 2015. p. 107-123.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 13. | | BOSCO, D. D.; SINSKI, I.; J. A. Fernando; QUECINI, V. Perfil transcricional em escala genômica de genes associados a respostas à temperatura em videira. In:CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 15., 2015; Conferência Brasil-Israel de Ciências de Plantas, 1., 2015, Foz do Iguaçú, PR. Anais... Foz do Iguaçú: SBFV, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 17. | | DAL BOSCO, D.; SINSKI, I.; RITSCHEL, P. S.; QUECINI, V. Residues of 2,4-Dichlorophenoxyacetic acid in plant-tissue culture glassware: their effects and a decontamination protocol. International Journal of Biotechnology & Biochemistry, Delhi, v. 6, n. 4, p. 617-624, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 18. | | STORCH, T. T.; FINATTO, T.; QUECINI, V.; ROMBALDI, C. V.; LAURENS, F.; GIRARDI, C. L. Associação entre a indução transcricional de uma α-l-arabinofuranosidase e etileno em polpa de maçã 'Gala' armazenada sob refrigeração. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, p. 848-851, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 20. | | QUECINI, V.; SINSKI, I.; RITSCHEL, P. S.; FAJARDO, T. V. M. Aiming at grapevines with increased resistance to pathogens, reaching structural genome modifications. In: Science & Wine World Congress, 1., 2019, Porto. Anais.... Porto, Pt: Alfândega Congress Centre, 8 a 10 maio de 2019.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 95 | |
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