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Registro Completo |
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
03/02/2014 |
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Data da última atualização: |
03/02/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LANNA FILHO, R.; SOUZA, R. M.; FERREIRA, A.; QUECINE, M. C.; ALVES, E.; AZEVEDO, J. L. de. |
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Afiliação: |
Roberto Lanna Filho, UFRGS; Ricardo Magela de Souza, UFLA; ANDERSON FERREIRA, CPAMT; Maria Carolina Quecine Verdi, USP-ESALQ; Eduardo Alves, UFLA; USP-ESALQ. |
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Título: |
Biocontrol activity of Bacillus against a GFP-marked Pseudomonas syringae pv. tomato on tomato phylloplane |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Australasian Plant Pathology, Holanda, v. 42, n. 6, p. 643-651, nov. 2013 |
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Volume: |
42 |
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Páginas: |
643-651 |
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ISSN: |
0815-3191 |
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DOI: |
http://dx.doi.org/10.1007/s13313-013-0233-z |
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Idioma: |
Inglês |
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Conteúdo: |
We report the biocontrol activity of the endophytic bacteria Bacillus pumilus and Bacillus amyloliquefacies against the plant pathogenic bacteriumPseudomonas syringae pv.tomato strain NS4 transformed with theGFPexpressing gene. P. s . pv. tomato strain NS4 was obtained from the transformation of P. s .pv.tomato wild-type strain NW with the plasmid pNKGFP containing GFP-cassette for chromosomal integration. The GFP-marked strain was tested for hypersensitivity and pathogenicity, as well as population studies on the phylloplane, to determine its epidemiology and survival. In all of the bioassays strain NS4 presented similar characteristics to the wild-type, and was hence chosen as the model strain for these studies with antagonistic endophytic bacterial strains. In the biocontrol experiments, tomato plants were preinoculated with the endophytic bacteria 4 days prior to inoculation withP. s .pv.tomatostrains. On the tomato phylloplane the P. s .pv.tomato (strains NW and NS4) populations were drastically reduced, and tomato leaves showed reduced numbers of bacterial speck lesions, comparable to the standard chemical treatment copper oxychloride. Additionally, under epifluorescence microscopy, few GFP-tagged cells of strain NS4 were observed colonizing important niches on the tomato phylloplane. However, leaves untreated with the antagonists presented a large number of GFP-tagged cell aggregates. Our results demonstrated that endophytic bacteria can also act efficiently on the biocontrol of bacterial speck when applied as a foliar spray on the leaves. In addition, we highlighted the use of GFP-marked strain NS4 as a model system to study biocontrol agent and pathogen interactions, and growth and development of the pathogen on the tomato leaf surface. MenosWe report the biocontrol activity of the endophytic bacteria Bacillus pumilus and Bacillus amyloliquefacies against the plant pathogenic bacteriumPseudomonas syringae pv.tomato strain NS4 transformed with theGFPexpressing gene. P. s . pv. tomato strain NS4 was obtained from the transformation of P. s .pv.tomato wild-type strain NW with the plasmid pNKGFP containing GFP-cassette for chromosomal integration. The GFP-marked strain was tested for hypersensitivity and pathogenicity, as well as population studies on the phylloplane, to determine its epidemiology and survival. In all of the bioassays strain NS4 presented similar characteristics to the wild-type, and was hence chosen as the model strain for these studies with antagonistic endophytic bacterial strains. In the biocontrol experiments, tomato plants were preinoculated with the endophytic bacteria 4 days prior to inoculation withP. s .pv.tomatostrains. On the tomato phylloplane the P. s .pv.tomato (strains NW and NS4) populations were drastically reduced, and tomato leaves showed reduced numbers of bacterial speck lesions, comparable to the standard chemical treatment copper oxychloride. Additionally, under epifluorescence microscopy, few GFP-tagged cells of strain NS4 were observed colonizing important niches on the tomato phylloplane. However, leaves untreated with the antagonists presented a large number of GFP-tagged cell aggregates. Our results demonstrated that endophytic bacteria can also act efficiently on the bi... Mostrar Tudo |
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Palavras-Chave: |
Bacterial Speck; Endophytic Bactéria. |
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Thesaurus Nal: |
Bacillus amyloliquefaciens; Bacillus pumilus; biological control; green fluorescent protein. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/96286/1/cpamt-ferreira-0815-3191-2013.pdf
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Marc: |
LEADER 02711naa a2200301 a 4500
001 1978335
005 2014-02-03
008 2013 bl uuuu u00u1 u #d
022 $a0815-3191
024 7 $ahttp://dx.doi.org/10.1007/s13313-013-0233-z$2DOI
100 1 $aLANNA FILHO, R.
245 $aBiocontrol activity of Bacillus against a GFP-marked Pseudomonas syringae pv. tomato on tomato phylloplane$h[electronic resource]
260 $c2013
300 $a643-651 42
490 $v42
520 $aWe report the biocontrol activity of the endophytic bacteria Bacillus pumilus and Bacillus amyloliquefacies against the plant pathogenic bacteriumPseudomonas syringae pv.tomato strain NS4 transformed with theGFPexpressing gene. P. s . pv. tomato strain NS4 was obtained from the transformation of P. s .pv.tomato wild-type strain NW with the plasmid pNKGFP containing GFP-cassette for chromosomal integration. The GFP-marked strain was tested for hypersensitivity and pathogenicity, as well as population studies on the phylloplane, to determine its epidemiology and survival. In all of the bioassays strain NS4 presented similar characteristics to the wild-type, and was hence chosen as the model strain for these studies with antagonistic endophytic bacterial strains. In the biocontrol experiments, tomato plants were preinoculated with the endophytic bacteria 4 days prior to inoculation withP. s .pv.tomatostrains. On the tomato phylloplane the P. s .pv.tomato (strains NW and NS4) populations were drastically reduced, and tomato leaves showed reduced numbers of bacterial speck lesions, comparable to the standard chemical treatment copper oxychloride. Additionally, under epifluorescence microscopy, few GFP-tagged cells of strain NS4 were observed colonizing important niches on the tomato phylloplane. However, leaves untreated with the antagonists presented a large number of GFP-tagged cell aggregates. Our results demonstrated that endophytic bacteria can also act efficiently on the biocontrol of bacterial speck when applied as a foliar spray on the leaves. In addition, we highlighted the use of GFP-marked strain NS4 as a model system to study biocontrol agent and pathogen interactions, and growth and development of the pathogen on the tomato leaf surface.
650 $aBacillus amyloliquefaciens
650 $aBacillus pumilus
650 $abiological control
650 $agreen fluorescent protein
653 $aBacterial Speck
653 $aEndophytic Bactéria
700 1 $aSOUZA, R. M.
700 1 $aFERREIRA, A.
700 1 $aQUECINE, M. C.
700 1 $aALVES, E.
700 1 $aAZEVEDO, J. L. de
773 $tAustralasian Plant Pathology, Holanda$gv. 42, n. 6, p. 643-651, nov. 2013
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Registro original: |
Embrapa Agrossilvipastoril (CPAMT) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/04/2003 |
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Data da última atualização: |
27/07/2017 |
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Autoria: |
GAZZONI, D. L. |
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Título: |
Cultura sadia. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Cultivar: grandes culturas, Pelotas, v.5, n. 49, p. 9-11, abr. 2003. |
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Idioma: |
Português |
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Thesagro: |
Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00311naa a2200121 a 4500
001 1448230
005 2017-07-27
008 2003 bl uuuu u00u1 u #d
100 1 $aGAZZONI, D. L.
245 $aCultura sadia.
260 $c2003
650 $aSoja
773 $tCultivar: grandes culturas, Pelotas$gv.5, n. 49, p. 9-11, abr. 2003.
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Embrapa Soja (CNPSO) |
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