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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sul. |
Data corrente: |
31/01/2012 |
Data da última atualização: |
31/01/2012 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SEVERO, P. de O.; QUADROS, F. L. F. de; BOAVISTA, L. da R.; DUTRA, G. M.; TRINDADE, J. P. P.; NUNES, G. D. |
Afiliação: |
PAULA DE OLIVEIRA SEVERO; FERNANDO LUIZ FERREIRA DE QUADROS; LIDIANE DA ROSA BOAVISTA; GABRIELA MACHADO DUTRA; JOSE PEDRO PEREIRA TRINDADE, CPPSUL; GISELE DUARTE NUNES. |
Título: |
Fitossociologia e índices de diversidade de pastagens naturais da região do Alto Camaquã, RS. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: JORNADA ACADÊMICA INTEGRADA, 26., 2011, Santa Maria. Anais... Santa Maria: UFSM, 2011. |
Idioma: |
Português |
Notas: |
JAI. |
Thesagro: |
Pastagem natural. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00617nam a2200181 a 4500 001 1913938 005 2012-01-31 008 2011 bl uuuu u00u1 u #d 100 1 $aSEVERO, P. de O. 245 $aFitossociologia e índices de diversidade de pastagens naturais da região do Alto Camaquã, RS.$h[electronic resource] 260 $aIn: JORNADA ACADÊMICA INTEGRADA, 26., 2011, Santa Maria. Anais... Santa Maria: UFSM$c2011 500 $aJAI. 650 $aPastagem natural 700 1 $aQUADROS, F. L. F. de 700 1 $aBOAVISTA, L. da R. 700 1 $aDUTRA, G. M. 700 1 $aTRINDADE, J. P. P. 700 1 $aNUNES, G. D.
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Embrapa Pecuária Sul (CPPSUL) |
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Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
26/04/2010 |
Data da última atualização: |
15/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
VIREQUE, A. A.; CAMARGO, L. S. de A.; SERAPIÃO, R. V.; ROSA E SILVA, A. A. M.; WATANABE, Y. F.; FERREIRA, E. M.; NAVARRO, P. A. A. S.; MARTINS, W. P.; FERRIANI, R. A. |
Afiliação: |
A. A. VIREQUE, USP; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; RAQUEL VARELLA SERAPIÃO, UFRRJ; A. A. M. ROSA E SILVA, Universidade de Brasília; Y. G. WATANABE, Vitrogen; E. M. FERREIRA, USP; P. A. A. S. NAVARRO, USP; W. P. MARTINS, USP; R. A. FERRIANI, USP. |
Título: |
Preimplantation development and expression of Hsp-70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Theriogenology, v. 71, n. 4, p. 620-627, 2009. |
DOI: |
https://doi.org/10.1016/j.theriogenology.2008.09.028 |
Idioma: |
Inglês |
Conteúdo: |
In vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in serum- and gonadotrophin-free medium supplemented with PVA or PVP, enriched with IGF-I and insulin, without altering post-cleavage development and relative abundance of some genes associated with stress and apoptosis. MenosIn vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in ... Mostrar Tudo |
Palavras-Chave: |
Bovine oocytes; In vitro maturation. |
Thesaurus NAL: |
embryogenesis. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/711876/1/Preimplantation-development-and-expression-of-Hsp-70-and-Bax-genes-in-bovine-blastocysts.pdf
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Marc: |
LEADER 02626naa a2200265 a 4500 001 1711876 005 2024-02-15 008 2009 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2008.09.028$2DOI 100 1 $aVIREQUE, A. A. 245 $aPreimplantation development and expression of Hsp-70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules. 260 $c2009 520 $aIn vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in serum- and gonadotrophin-free medium supplemented with PVA or PVP, enriched with IGF-I and insulin, without altering post-cleavage development and relative abundance of some genes associated with stress and apoptosis. 650 $aembryogenesis 653 $aBovine oocytes 653 $aIn vitro maturation 700 1 $aCAMARGO, L. S. de A. 700 1 $aSERAPIÃO, R. V. 700 1 $aROSA E SILVA, A. A. M. 700 1 $aWATANABE, Y. F. 700 1 $aFERREIRA, E. M. 700 1 $aNAVARRO, P. A. A. S. 700 1 $aMARTINS, W. P. 700 1 $aFERRIANI, R. A. 773 $tTheriogenology$gv. 71, n. 4, p. 620-627, 2009.
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