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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Tabuleiros Costeiros. |
Data corrente: |
07/12/2022 |
Data da última atualização: |
22/12/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
OLIVEIRA, M. B. de; MOLINA, J. C. J.; SILVA, R. S. da; RAMOS, A. F.; PURDY, P. H.; AZEVEDO, H. C. |
Afiliação: |
MATHEUS BATISTA DE OLIVEIRA; JULIO CONSTANTINO JERI MOLINA; REBECA SANTOS DA SILVA; ALEXANDRE FLORIANI RAMOS, Cenargen; PHILLIP HAMILTON PURDY; HYMERSON COSTA AZEVEDO, CPATC. |
Título: |
Effects of cell concentration during cryopreservation on the post-thaw quality of Santa Inês ram sperm. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
CryoLetters, v. 43, n. 6, p. 357?367, 2022. |
DOI: |
https://doi.org/10.54680/fr22610110812 |
Idioma: |
Inglês |
Conteúdo: |
Non-surgical artificial insemination techniques for sheep may benefit from larger numbers of sperm in the insemination dose because the ewe?s cervix is convoluted and often cannot be traversed with an insemination gun resulting in deposition of the sperm at the os cervix. OBJECTIVE: To compare a range of sperm concentrations when cryopreserving semen from Santa Inês rams and determine the effects of this on post-thaw quality. MATERIALS AND METHODS: One ejaculate from each ram (n = 10) was diluted to four sperm concentrations to obtain the following groups: G?400, G?800, G?1200, and G?1600 x 106 sperm/mL. The semen samples were packaged in 0.25 mL straws,cooled to 5 ºC, cryopreserved in liquid nitrogen vapor, thawed in a water bath (40 ºC/20 s), and were analyzed for computerized kinetics, capacitation and acrosome integrity, and plasma membrane integrity of sperm. RESULTS: The G?400 treatment resulted in samples with the highest linearity and progressive motion (P < 0.05) and had significantly greater plasma membrane integrity, and lower capacitation and acrosome reaction rates compared to G-1600 (P < 0.05). Overall, use of the G?400 treatment resulted in better kinetics, less plasma membrane damage and less early capacitation. However, despite reducing the ejaculate yield and increasing the costs of the semen freezing process, the G?800 and G?1200 treatments make a greater absolute number of sperm with good kinetics, plasma membrane integrity and capacitation status available. CONCLUSION: Ram sperm concentration impacts cryopreservation, and higher concentrations may be advantageous if a single artificial insemination protocol is desirable. MenosNon-surgical artificial insemination techniques for sheep may benefit from larger numbers of sperm in the insemination dose because the ewe?s cervix is convoluted and often cannot be traversed with an insemination gun resulting in deposition of the sperm at the os cervix. OBJECTIVE: To compare a range of sperm concentrations when cryopreserving semen from Santa Inês rams and determine the effects of this on post-thaw quality. MATERIALS AND METHODS: One ejaculate from each ram (n = 10) was diluted to four sperm concentrations to obtain the following groups: G?400, G?800, G?1200, and G?1600 x 106 sperm/mL. The semen samples were packaged in 0.25 mL straws,cooled to 5 ºC, cryopreserved in liquid nitrogen vapor, thawed in a water bath (40 ºC/20 s), and were analyzed for computerized kinetics, capacitation and acrosome integrity, and plasma membrane integrity of sperm. RESULTS: The G?400 treatment resulted in samples with the highest linearity and progressive motion (P < 0.05) and had significantly greater plasma membrane integrity, and lower capacitation and acrosome reaction rates compared to G-1600 (P < 0.05). Overall, use of the G?400 treatment resulted in better kinetics, less plasma membrane damage and less early capacitation. However, despite reducing the ejaculate yield and increasing the costs of the semen freezing process, the G?800 and G?1200 treatments make a greater absolute number of sperm with good kinetics, plasma membrane integrity and capacitation status availab... Mostrar Tudo |
Palavras-Chave: |
Ovino Santa Iês. |
Thesagro: |
Carneiro; Esperma; Inseminação; Inseminação Artificial; Ovelha; Ovino. |
Thesaurus Nal: |
Rams; Sperm concentration. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02544naa a2200301 a 4500 001 2150268 005 2022-12-22 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.54680/fr22610110812$2DOI 100 1 $aOLIVEIRA, M. B. de 245 $aEffects of cell concentration during cryopreservation on the post-thaw quality of Santa Inês ram sperm.$h[electronic resource] 260 $c2022 520 $aNon-surgical artificial insemination techniques for sheep may benefit from larger numbers of sperm in the insemination dose because the ewe?s cervix is convoluted and often cannot be traversed with an insemination gun resulting in deposition of the sperm at the os cervix. OBJECTIVE: To compare a range of sperm concentrations when cryopreserving semen from Santa Inês rams and determine the effects of this on post-thaw quality. MATERIALS AND METHODS: One ejaculate from each ram (n = 10) was diluted to four sperm concentrations to obtain the following groups: G?400, G?800, G?1200, and G?1600 x 106 sperm/mL. The semen samples were packaged in 0.25 mL straws,cooled to 5 ºC, cryopreserved in liquid nitrogen vapor, thawed in a water bath (40 ºC/20 s), and were analyzed for computerized kinetics, capacitation and acrosome integrity, and plasma membrane integrity of sperm. RESULTS: The G?400 treatment resulted in samples with the highest linearity and progressive motion (P < 0.05) and had significantly greater plasma membrane integrity, and lower capacitation and acrosome reaction rates compared to G-1600 (P < 0.05). Overall, use of the G?400 treatment resulted in better kinetics, less plasma membrane damage and less early capacitation. However, despite reducing the ejaculate yield and increasing the costs of the semen freezing process, the G?800 and G?1200 treatments make a greater absolute number of sperm with good kinetics, plasma membrane integrity and capacitation status available. CONCLUSION: Ram sperm concentration impacts cryopreservation, and higher concentrations may be advantageous if a single artificial insemination protocol is desirable. 650 $aRams 650 $aSperm concentration 650 $aCarneiro 650 $aEsperma 650 $aInseminação 650 $aInseminação Artificial 650 $aOvelha 650 $aOvino 653 $aOvino Santa Iês 700 1 $aMOLINA, J. C. J. 700 1 $aSILVA, R. S. da 700 1 $aRAMOS, A. F. 700 1 $aPURDY, P. H. 700 1 $aAZEVEDO, H. C. 773 $tCryoLetters$gv. 43, n. 6, p. 357?367, 2022.
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Registros recuperados : 7 | |
3. | | DIAS, I. D. M.; OLIVEIRA, D. A.; OLIVEIRA, M. B. de; RAMOS, A. F.; PURDY, P. H.; AZEVEDO, H. C. Avaliação de diferentes momentos de inseminação artificial transcervical em tempo fixo com uso de sêmen congelado em ovinos Santa Inês. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA TABULEIROS COSTEIROS, 9., 2019, Aracaju. Anais... Aracaju: Embrapa Tabuleiros Costeiros; Brasília, DF: Embrapa, 2019. Editor Técnico: Ronaldo Souza Resende. p. 22. Resumo.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Tabuleiros Costeiros. |
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4. | | BANDEIRA, A. M. P.; MATOS, J. E.; MARIA, A. N.; CARNEIRO, P. C. F.; PURDY, P. H.; AZEVEDO, H. C. The effects of gelatin supplementation prior to cooling on ram semen quality and fertility. Animal Reproduction, v. 15, n. 1, p. 23-28, Jan./Mar. 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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5. | | OLIVEIRA, M. B. de; MOLINA, J. C. J.; SILVA, R. S. da; DIAS, I. D. M.; RAMOS, A. F.; PURDY, P. H.; AZEVEDO, H. C. Qualidade do sêmen de carneiros Santa Inês congelado com diferentes concentrações espermáticas. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA TABULEIROS COSTEIROS, 9., 2019, Aracaju. Anais... Aracaju: Embrapa Tabuleiros Costeiros; Brasília, DF: Embrapa, 2019. Editor Técnico: Ronaldo Souza Resende. p. 21.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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6. | | BLACKBURN, H. D.; PAIVA, S. R.; WILDEUS, S.; GETZ, W.; WALDRON, D.; STOBART, R.; BIXBY, D.; PURDY, P. H.; WELS, C.; SPILLER, S.; BROWN, M. Genetic structure and diversity among sheep breeds in the United States: identification of the major gene pools. Journal of Animal Science, v.89, p. 2336-2348, 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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7. | | OLIVEIRA, M. B. de; MOLINA, J. C. J.; SILVA, R. S. da; RAMOS, A. F.; PURDY, P. H.; AZEVEDO, H. C. Effects of cell concentration during cryopreservation on the post-thaw quality of Santa Inês ram sperm. CryoLetters, v. 43, n. 6, p. 357?367, 2022.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Tabuleiros Costeiros. |
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Registros recuperados : 7 | |
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