|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
31/05/2016 |
Data da última atualização: |
11/10/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GONÇALVES, F. J.; FILIPPI, M. C. C. de; LOBO, V. L. S.; ARAÚJO, L. G.; SILVA, G. B.; COELHO, A. S. G.; PRABHU, A. S. |
Afiliação: |
FABIO JOSE GONÇALVES; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; VALACIA LEMES DA SILVA LOBO, CNPAF; LEILA GARCÊS ARAÚJO, UFG; GISELE BARATA SILVA, UFRA; ALEXANDRE SIQUEIRA GUEDES COELHO, UFV; ANNE SITARAMA PRABHU, CNPAF. |
Título: |
Polymorphism detection by microsatellite markers in a Magnaporthe oryzae population from different geographical areas of Brazil. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Journal of Phytopathology, v. 164, n. 9, p. 620-630, Sept. 2016. |
ISSN: |
0931-1785 |
DOI: |
10.1111/jph.12485 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to examine Brazilian M. oryzae populations using 18 microsatellites. Fifty cultivars were sown in plastic trays for the pathotyping of 847 isolates. The DNA of 494 isolates was extracted and purified using the modified Doyle and Doyle method, the genetic structure was determined by the software Structure, and the actual number was selected from the prediction method based on the K values. Nei's genetic distance among the subpopulations was determined with the aid of the program Genetix, and the amova was performed with the program Arlequin. Out of 847 inoculated monosporic isolates, 528 infected their respective cultivars; of the 528 isolates pathotyped, there was a prevalence of group IA and pathotype IF-1, which was the most frequent pathotype in the rice production areas of Brazil. The Bayesian clustering analysis indicated that 19 was the optimal value of K; this value was the lowest standard deviation and log (ln K) closest to zero, which predicted the 494 isolates of M. oryzae that were selected for molecular studies to be grouped into 19 subpopulations. The AMOVA detected a 37.13% variability within the 19 subpopulations and 62.87% variability among the subpopulations. The polymorphic information content (PIC) ranged from 0 to 0.756. Thirty three rare alleles were found distributed among 15 out of 19 subpopulations. The Margalef index ranged from 38.69 to 79.21 for all 18 analysed locus. The results indicated that the identification of different blast resistance genes must consider the composition of each subpopulation and that the identification is most effective when performed within a subpopulation and then between subpopulations. MenosThis study aimed to examine Brazilian M. oryzae populations using 18 microsatellites. Fifty cultivars were sown in plastic trays for the pathotyping of 847 isolates. The DNA of 494 isolates was extracted and purified using the modified Doyle and Doyle method, the genetic structure was determined by the software Structure, and the actual number was selected from the prediction method based on the K values. Nei's genetic distance among the subpopulations was determined with the aid of the program Genetix, and the amova was performed with the program Arlequin. Out of 847 inoculated monosporic isolates, 528 infected their respective cultivars; of the 528 isolates pathotyped, there was a prevalence of group IA and pathotype IF-1, which was the most frequent pathotype in the rice production areas of Brazil. The Bayesian clustering analysis indicated that 19 was the optimal value of K; this value was the lowest standard deviation and log (ln K) closest to zero, which predicted the 494 isolates of M. oryzae that were selected for molecular studies to be grouped into 19 subpopulations. The AMOVA detected a 37.13% variability within the 19 subpopulations and 62.87% variability among the subpopulations. The polymorphic information content (PIC) ranged from 0 to 0.756. Thirty three rare alleles were found distributed among 15 out of 19 subpopulations. The Margalef index ranged from 38.69 to 79.21 for all 18 analysed locus. The results indicated that the identification of different blast... Mostrar Tudo |
Thesagro: |
Arroz; Brusone; Marcador molecular; Oryza sativa. |
Thesaurus Nal: |
Blast disease; Magnaporthe oryzae; Microsatellite repeats; Rice. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02619naa a2200313 a 4500 001 2045924 005 2016-10-11 008 2016 bl uuuu u00u1 u #d 022 $a0931-1785 024 7 $a10.1111/jph.12485$2DOI 100 1 $aGONÇALVES, F. J. 245 $aPolymorphism detection by microsatellite markers in a Magnaporthe oryzae population from different geographical areas of Brazil.$h[electronic resource] 260 $c2016 520 $aThis study aimed to examine Brazilian M. oryzae populations using 18 microsatellites. Fifty cultivars were sown in plastic trays for the pathotyping of 847 isolates. The DNA of 494 isolates was extracted and purified using the modified Doyle and Doyle method, the genetic structure was determined by the software Structure, and the actual number was selected from the prediction method based on the K values. Nei's genetic distance among the subpopulations was determined with the aid of the program Genetix, and the amova was performed with the program Arlequin. Out of 847 inoculated monosporic isolates, 528 infected their respective cultivars; of the 528 isolates pathotyped, there was a prevalence of group IA and pathotype IF-1, which was the most frequent pathotype in the rice production areas of Brazil. The Bayesian clustering analysis indicated that 19 was the optimal value of K; this value was the lowest standard deviation and log (ln K) closest to zero, which predicted the 494 isolates of M. oryzae that were selected for molecular studies to be grouped into 19 subpopulations. The AMOVA detected a 37.13% variability within the 19 subpopulations and 62.87% variability among the subpopulations. The polymorphic information content (PIC) ranged from 0 to 0.756. Thirty three rare alleles were found distributed among 15 out of 19 subpopulations. The Margalef index ranged from 38.69 to 79.21 for all 18 analysed locus. The results indicated that the identification of different blast resistance genes must consider the composition of each subpopulation and that the identification is most effective when performed within a subpopulation and then between subpopulations. 650 $aBlast disease 650 $aMagnaporthe oryzae 650 $aMicrosatellite repeats 650 $aRice 650 $aArroz 650 $aBrusone 650 $aMarcador molecular 650 $aOryza sativa 700 1 $aFILIPPI, M. C. C. de 700 1 $aLOBO, V. L. S. 700 1 $aARAÚJO, L. G. 700 1 $aSILVA, G. B. 700 1 $aCOELHO, A. S. G. 700 1 $aPRABHU, A. S. 773 $tJournal of Phytopathology$gv. 164, n. 9, p. 620-630, Sept. 2016.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
08/09/2003 |
Data da última atualização: |
28/12/2018 |
Autoria: |
FRANCO, D. F.; PETRINI, J. A. |
Título: |
Produção de semente genética de arroz irrigado através do sistema de transplante de mudas. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Pelotas: Embrapa Clima Temperado, 2002. |
Páginas: |
3 p. |
Série: |
(Embrapa Clima Temperado. Comunicado técnico, 60) |
Idioma: |
Português |
Palavras-Chave: |
roz. |
Thesagro: |
Arroz; Arroz Vermelho; Genética; Muda; Produção; Semente; Transplante. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31543/1/comunicado60.pdf
|
Marc: |
LEADER 00636nam a2200229 a 4500 001 1744405 005 2018-12-28 008 2002 bl uuuu u0uu1 u #d 100 1 $aFRANCO, D. F. 245 $aProdução de semente genética de arroz irrigado através do sistema de transplante de mudas. 260 $aPelotas: Embrapa Clima Temperado$c2002 300 $a3 p. 490 $a(Embrapa Clima Temperado. Comunicado técnico, 60) 650 $aArroz 650 $aArroz Vermelho 650 $aGenética 650 $aMuda 650 $aProdução 650 $aSemente 650 $aTransplante 653 $aroz 700 1 $aPETRINI, J. A.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Clima Temperado (CPACT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|