Data/hora: 01/06/2024 11:28:15

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais.
Data corrente: | 05/08/2016
Data da última atualização: | 22/09/2016
Autoria: | QUEIROZ, P. R.; MARTINS, E. S.; KLAUTAU, N.; LIMA, L.; PRACA, L.; PONTES, R. G. M. S. de.
Afiliação: | PAULO ROBERTO QUEIROZ, Instituto Mato-Grossense do Algodão; ERICA SOCARES MARTINS, Instituto Mato-Grossence do Algodão; NAZARÉ KLAUTAU, UNB; LUZIA HELENA CORREA LIMA, Cenargen; LILIAN BOTELHO PRACA, Cenargen; ROSE GOMES MONNERAT SOLON DE PONTES, Cenargen.
Título: | Identification of the B, Q, and native brazilian biotypes of the Bemisia tabaci species complex using Scar markers.
Ano de publicação: | 2016
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília v. 51, n. 5, p. 555-562, maio 2016.
DOI: | 10.1590/S0100-204X2016000500016
Idioma: | Inglês
Conteúdo: | The objective of this work was to develop sequence?characterized amplified region (Scar) markers to identify the B, Q, and native Brazilian biotypes of the sweet potato whitefly [Bemisia tabaci (Hemiptera: Aleyrodidae)]. Random amplified polymorphic DNA (RAPD) amplification products, exclusive to the B and Brazilian biotypes, were selected after the analysis of 12,000 samples, in order to design a specific Scar primer set. The BT?B1 and BT?B3 Scar markers, used to detect the B biotype, produced PCR fragments of 850 and 582 bp, respectively. The BT?BR1 Scar marker, used to identify the Brazilian biotype, produced a PCR fragment of 700 bp. The Scar markers were tested against the Q biotype, and a flowchart was proposed to indicate the decision steps to use these primers, in order to correctly discriminate the biotypes. This procedure allowed to identify the biotypes that occur in field samples, such as the B biotype. The used set of primers allowed to discriminate the B, Q, and native Brazilian biotypes of B. tabaci. These primers can be successfully used to identify the B biotype of B. tabaci from field samples, showing only one specific biotype present in all cultures.
Palavras-Chave: | Amostra de campo; Field samples.
Thesagro: | Bemisia Tabaci; Marcador molecular; Mosca branca.
Thesaurus Nal: | Aleyrodidae; Genetic markers; Products and commodities.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/146114/1/Identification-of-the-B-Q-and-native.pdf
Registro original: | Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)

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