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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
07/04/2004 |
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Autoria: |
SCHÄRER-HERNÁNDEZ, NG; FREY, PM; POTRYKUS, I; PUONTI-KAERLAS, J. |
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Título: |
Towards African cassava mosaic virus resistance in cassava. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v.17, p.43, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Cassava (Manihot esculenta Crantz) is one of the most important staple crops in the tropics. It is growm for its tuberous roots, which provide food for over 500 million people. Because of its hardiness, tolerance to adverse environmental conditions and its flexible harvesting time, it plays and integral part in food security in developing countries. Cassava yields are, however, severely reduced by various insect pests and diseases. Traditional breeding of cassava is difficult, so biotechnology provides a valuable tool for genetic crop improvement. In our laboratory a method for producing transgenic cassava plants has been developed by combining direct shoot organogenesis from somatic cotyledon explants and Agrobacterium-mediated gene transfer. We have started applying the transformation technique transfering agriculturally relivant genes to cassava. The most devastating disease in all African food crops is African cassava mosaic virus disease. It is caused by African cassava Mosaic Virus (ACMV) and can cause local losses of up to 80%. So far there is no method available to protect the plants in the field from the virus. Our aim is to develop and evaluate methods for producing genetically improved cassava varieties resistant to ACMV, which will be able to grow and produce high yields under severe ACMV infection pressures. In order to achieve the suppression of virus replication, the effect of the expression of viral antisense RNAs as 3'-UTRs of a selectable marker gene, of truncated viral gene products and of defective interfering DNA will be assessed. MenosAbstract: Cassava (Manihot esculenta Crantz) is one of the most important staple crops in the tropics. It is growm for its tuberous roots, which provide food for over 500 million people. Because of its hardiness, tolerance to adverse environmental conditions and its flexible harvesting time, it plays and integral part in food security in developing countries. Cassava yields are, however, severely reduced by various insect pests and diseases. Traditional breeding of cassava is difficult, so biotechnology provides a valuable tool for genetic crop improvement. In our laboratory a method for producing transgenic cassava plants has been developed by combining direct shoot organogenesis from somatic cotyledon explants and Agrobacterium-mediated gene transfer. We have started applying the transformation technique transfering agriculturally relivant genes to cassava. The most devastating disease in all African food crops is African cassava mosaic virus disease. It is caused by African cassava Mosaic Virus (ACMV) and can cause local losses of up to 80%. So far there is no method available to protect the plants in the field from the virus. Our aim is to develop and evaluate methods for producing genetically improved cassava varieties resistant to ACMV, which will be able to grow and produce high yields under severe ACMV infection pressures. In order to achieve the suppression of virus replication, the effect of the expression of viral antisense RNAs as 3'-UTRs of a selectable marker g... Mostrar Tudo |
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Categoria do assunto: |
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LEADER 02051naa a2200157 a 4500
001 1651824
005 2004-04-07
008 1998 bl uuuu u00u1 u #d
100 1 $aSCHÄRER-HERNÁNDEZ, NG
245 $aTowards African cassava mosaic virus resistance in cassava.
260 $c1998
520 $aAbstract: Cassava (Manihot esculenta Crantz) is one of the most important staple crops in the tropics. It is growm for its tuberous roots, which provide food for over 500 million people. Because of its hardiness, tolerance to adverse environmental conditions and its flexible harvesting time, it plays and integral part in food security in developing countries. Cassava yields are, however, severely reduced by various insect pests and diseases. Traditional breeding of cassava is difficult, so biotechnology provides a valuable tool for genetic crop improvement. In our laboratory a method for producing transgenic cassava plants has been developed by combining direct shoot organogenesis from somatic cotyledon explants and Agrobacterium-mediated gene transfer. We have started applying the transformation technique transfering agriculturally relivant genes to cassava. The most devastating disease in all African food crops is African cassava mosaic virus disease. It is caused by African cassava Mosaic Virus (ACMV) and can cause local losses of up to 80%. So far there is no method available to protect the plants in the field from the virus. Our aim is to develop and evaluate methods for producing genetically improved cassava varieties resistant to ACMV, which will be able to grow and produce high yields under severe ACMV infection pressures. In order to achieve the suppression of virus replication, the effect of the expression of viral antisense RNAs as 3'-UTRs of a selectable marker gene, of truncated viral gene products and of defective interfering DNA will be assessed.
700 1 $aFREY, PM
700 1 $aPOTRYKUS, I
700 1 $aPUONTI-KAERLAS, J.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv.17, p.43, nov., 1998. Suplemento.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
25/11/2022 |
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Data da última atualização: |
25/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CARVALHO, M. H. de; ROSA, S. D. V. F. da; COELHO, S. V. B.; GUIMARÃES, C. C.; MARTINS, R. de S.; CLEMENTE, A. da C. S.; PAIVA, L. V. |
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Afiliação: |
MAYARA HOLANDA DE CARVALHO; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; STEFÂNIA VIIAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; CRISTIANE CARVALHO GUIMARÃES, UNIVERSIDADE ESTADUAL PAULISTA; RAYANA DE SÁ MARTINS, INSTITUTO DE BOTÂNICA; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, ONSEEDS TREINAMENTOS; LUCIANO VILELA PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS. |
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Título: |
Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Acta Scientiarum: Agronomy, v. 45, e56908, 2023. |
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DOI: |
https://doi.org/10.4025/actasciagron.v45i1.56908 |
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Idioma: |
Inglês |
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Conteúdo: |
The reduced longevity of coffee seeds has been attributed to their sensitivity to desiccation. Studies related to gene expression and enzyme activity in coffee seeds under drying are important for understanding the effects of drying on their physiological quality. The aim of this study was to investigate the molecular aspects of seeds under different drying methods and associate them with physiological quality. Coffee seeds with different water contents were dried both slowly and rapidly. Enzymatic activity was analysed, as well as the expression of genes that encode the enzymes superoxide dismutase, catalase, peroxiredoxins, isocitrate lyase, and endo-ß-mannanase. There was a significant effect of drying speed and final water content on enzyme activity and on the expression of the different genes analysed. In seeds under rapid drying, there was greater expression of the genes that encode the enzymes catalase and endo-ß-mannanase. Greater expression of the 1 CYS PRX and SOD genes and greater activity of the ICL isoenzymes were found in seeds with superior physiological quality, but greater activity of the endo-β-mannanase and CAT enzymes occurred in seeds with lower physiological quality. |
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Thesaurus NAL: |
Coffea arabica var. arabica; Drying; Enzyme activity; Oxidative stress. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148771/1/Carvalho-et-al-2023-Drying-of-arabica-coffee-and-its-effect-on-the-gene-expression-and-activity-of-enzymes-linked-to-seed-physiological-quality.pdf
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Marc: |
LEADER 02045naa a2200253 a 4500
001 2148771
005 2022-11-25
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4025/actasciagron.v45i1.56908$2DOI
100 1 $aCARVALHO, M. H. de
245 $aDrying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.$h[electronic resource]
260 $c2023
520 $aThe reduced longevity of coffee seeds has been attributed to their sensitivity to desiccation. Studies related to gene expression and enzyme activity in coffee seeds under drying are important for understanding the effects of drying on their physiological quality. The aim of this study was to investigate the molecular aspects of seeds under different drying methods and associate them with physiological quality. Coffee seeds with different water contents were dried both slowly and rapidly. Enzymatic activity was analysed, as well as the expression of genes that encode the enzymes superoxide dismutase, catalase, peroxiredoxins, isocitrate lyase, and endo-ß-mannanase. There was a significant effect of drying speed and final water content on enzyme activity and on the expression of the different genes analysed. In seeds under rapid drying, there was greater expression of the genes that encode the enzymes catalase and endo-ß-mannanase. Greater expression of the 1 CYS PRX and SOD genes and greater activity of the ICL isoenzymes were found in seeds with superior physiological quality, but greater activity of the endo-β-mannanase and CAT enzymes occurred in seeds with lower physiological quality.
650 $aCoffea arabica var. arabica
650 $aDrying
650 $aEnzyme activity
650 $aOxidative stress
700 1 $aROSA, S. D. V. F. da
700 1 $aCOELHO, S. V. B.
700 1 $aGUIMARÃES, C. C.
700 1 $aMARTINS, R. de S.
700 1 $aCLEMENTE, A. da C. S.
700 1 $aPAIVA, L. V.
773 $tActa Scientiarum: Agronomy$gv. 45, e56908, 2023.
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Embrapa Café (CNPCa) |
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