Data/hora: 04/06/2024 17:24:06

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	15/04/2004	
Data da última atualização: 	15/04/2004	
Autoria: 	LEGRIS, G.; POTRYKUS, I.; PUONTI-KAERLAS, J.	
Título: 	Engineering insect resistance in cassava.	
Ano de publicação: 	1998	
Fonte/Imprenta: 	Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p. 30, nov., 1998. Suplemento.	
Idioma: 	Inglês	
Conteúdo: 	Abstract: Insect pests can be a severe problem for cassava farmers in Latin America. Recently, stem borers, difficult to combat chemically, have caused increasing to cassava production. Transfer of insecticidal genes e.g. the Bt gene from Bacillus thuringiensis into plants allows them to protect themselves against insect attacks. Lepidopteran insects are susceptible to Bt, and hence production of transgenic cassava containing Bt genes should allow a pest control method that is environmentally and economically sustainable. Both Agrobacterium-mediated transformation and PIG (Particle Inflow Gun) were used to transform cassava with constructs carrying the Bt gene. No shoots could be regenerated from the explants transformed by Agrobacterium-mediated transformation, even, when the transformation conditions were optimised. So far more than 60 plants from explants cotransformed with plasmids carrying the Bt and hpt genes, have been regenerated, and the presence of the transgenes in some of the regenerated shoots has been demonstrated by PCR. Preliminary analysis using PCR shows that the cotransformation frequency is about 50%. The material is Currently being multiplied for molecular analysis.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (20957-1) - ADD - AP - -- - 633.68205 -  -  - DIS