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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
09/01/2024 |
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Data da última atualização: |
09/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GEROMEL, C.; FERREIRA, L. P.; GUERREIRO, S. M. C.; CAVALARI, A. A.; POT, D.; PEREIRA, L. F. P.; LEROY, T.; VIEIRA, L. G. E.; MAZZAFERA, P.; MARRACCINI, P. |
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Afiliação: |
CLARA GEROMEL, UNIVERSIDADE ESTADUAL DE CAMPINAS; LÚCIA PIRES FERREIRA, INSTITUTO AGRONÔMICO DO PARANÁ; SANDRA MARIA CARMELO GUERREIRO, UNIVERSIDADE ESTADUAL DE CAMPINAS; ALINE ANDRÉIA CAVALARI, UNIVERSIDADE ESTADUAL DE CAMPINAS; DAVID POT, INSTITUTO AGRONÔMICO DO PARANÁ; LUIZ FILIPE PROTASIO PEREIRA, CNPCa; THIERRY LEROY, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRONÔMICA PARA O DESENVOLVIMENTO; LUIZ GONZAGA ESTEVES VIEIRA, INSTITUTO AGRONÔMICO DO PARANÁ; PAULO MAZZAFERA, UNIVERSIDADE ESTADUAL DE CAMPINAS; PIERRE MARRACCINI, CENTRO DE COOPERAÇÃO INTERNACIONAL EM PESQUISA AGRONÔMICA PARA O DESENVOLVIMENTO. |
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Título: |
Biochemical and genomic analysis of sucrose metabolism during coffee (Coffea arabica) fruit development. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Journal of Experimental Botany, v. 57, n. 12, p. 3243-3258, 2006. |
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DOI: |
https://www.doi.org/10.1093/jxb/erl084 |
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Idioma: |
Inglês |
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Conteúdo: |
Sucrose metabolism and the role of sucrose synthase were investigated in the fruit tissues (pericarp, perisperm, and endosperm) of Coffea arabica during development. Acid invertase, sucrose phosphate synthase, and sucrose synthase activities were monitored and compared with the levels of sucrose and reducing sugars. Among these enzymes, sucrose synthase showed the highest activities during the last stage of endosperm and pericarp development and this activity paralleled closely the accumulation of sucrose in these tissues at this stage. Carbon partitioning in fruits was studied by pulse-chase experiments with C-14-sugars and revealed high rates of sucrose turnover in perisperm and endosperm tissues. Additional feeding experiments with (CO2)-C-14 showed that leaf photosynthesis contributed more to seed development than the pericarp in terms of photosynthate supply to the endosperm. Sugar analysis, feeding experiments, and histological studies indicated that the perisperm plays an important role in this downloading process. It was observed that the perisperm presents a transient accumulation of starch which is degraded as the seed develops. Two full-length cDNAs (CaSUS1 and CaSUS2) and the complete gene sequence of the latter were also isolated. They encode sucrose synthase isoforms that are phylogenetically distinct, indicating their involvement in different physiological functions during cherry development. Contrasting expression patterns were observed for CaSUS1 and CaSUS2 in perisperm, endosperm, and pericarp tissues: CaSUS1 mRNAs accumulated mainly during the early development of perisperm and endosperm, as well as during pericarp growing phases, whereas those of CaSUS2 paralleled sucrose synthase activity in the last weeks of pericarp and endosperm development. Taken together, these results indicate that sucrose synthase plays an important role in sugar metabolism during sucrose accumulation in the coffee fruit. MenosSucrose metabolism and the role of sucrose synthase were investigated in the fruit tissues (pericarp, perisperm, and endosperm) of Coffea arabica during development. Acid invertase, sucrose phosphate synthase, and sucrose synthase activities were monitored and compared with the levels of sucrose and reducing sugars. Among these enzymes, sucrose synthase showed the highest activities during the last stage of endosperm and pericarp development and this activity paralleled closely the accumulation of sucrose in these tissues at this stage. Carbon partitioning in fruits was studied by pulse-chase experiments with C-14-sugars and revealed high rates of sucrose turnover in perisperm and endosperm tissues. Additional feeding experiments with (CO2)-C-14 showed that leaf photosynthesis contributed more to seed development than the pericarp in terms of photosynthate supply to the endosperm. Sugar analysis, feeding experiments, and histological studies indicated that the perisperm plays an important role in this downloading process. It was observed that the perisperm presents a transient accumulation of starch which is degraded as the seed develops. Two full-length cDNAs (CaSUS1 and CaSUS2) and the complete gene sequence of the latter were also isolated. They encode sucrose synthase isoforms that are phylogenetically distinct, indicating their involvement in different physiological functions during cherry development. Contrasting expression patterns were observed for CaSUS1 and CaSUS2 ... Mostrar Tudo |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Endosperm; Gene expression; Sucrose synthase. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160617/1/Biochemical-genomic-analysis.pdf
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Marc: |
LEADER 02824naa a2200289 a 4500
001 2160617
005 2024-01-09
008 2006 bl uuuu u00u1 u #d
024 7 $ahttps://www.doi.org/10.1093/jxb/erl084$2DOI
100 1 $aGEROMEL, C.
245 $aBiochemical and genomic analysis of sucrose metabolism during coffee (Coffea arabica) fruit development.$h[electronic resource]
260 $c2006
520 $aSucrose metabolism and the role of sucrose synthase were investigated in the fruit tissues (pericarp, perisperm, and endosperm) of Coffea arabica during development. Acid invertase, sucrose phosphate synthase, and sucrose synthase activities were monitored and compared with the levels of sucrose and reducing sugars. Among these enzymes, sucrose synthase showed the highest activities during the last stage of endosperm and pericarp development and this activity paralleled closely the accumulation of sucrose in these tissues at this stage. Carbon partitioning in fruits was studied by pulse-chase experiments with C-14-sugars and revealed high rates of sucrose turnover in perisperm and endosperm tissues. Additional feeding experiments with (CO2)-C-14 showed that leaf photosynthesis contributed more to seed development than the pericarp in terms of photosynthate supply to the endosperm. Sugar analysis, feeding experiments, and histological studies indicated that the perisperm plays an important role in this downloading process. It was observed that the perisperm presents a transient accumulation of starch which is degraded as the seed develops. Two full-length cDNAs (CaSUS1 and CaSUS2) and the complete gene sequence of the latter were also isolated. They encode sucrose synthase isoforms that are phylogenetically distinct, indicating their involvement in different physiological functions during cherry development. Contrasting expression patterns were observed for CaSUS1 and CaSUS2 in perisperm, endosperm, and pericarp tissues: CaSUS1 mRNAs accumulated mainly during the early development of perisperm and endosperm, as well as during pericarp growing phases, whereas those of CaSUS2 paralleled sucrose synthase activity in the last weeks of pericarp and endosperm development. Taken together, these results indicate that sucrose synthase plays an important role in sugar metabolism during sucrose accumulation in the coffee fruit.
650 $aEndosperm
650 $aGene expression
650 $aSucrose synthase
650 $aCoffea Arábica
700 1 $aFERREIRA, L. P.
700 1 $aGUERREIRO, S. M. C.
700 1 $aCAVALARI, A. A.
700 1 $aPOT, D.
700 1 $aPEREIRA, L. F. P.
700 1 $aLEROY, T.
700 1 $aVIEIRA, L. G. E.
700 1 $aMAZZAFERA, P.
700 1 $aMARRACCINI, P.
773 $tJournal of Experimental Botany$gv. 57, n. 12, p. 3243-3258, 2006.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
11/09/2003 |
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Data da última atualização: |
28/02/2013 |
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Autoria: |
MELO, J. T. de; GONCALVES, A. N. |
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Título: |
Inibidores de germinacao em frutos e sementes de pequi. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Planaltina: Embrapa Cerrados, 2001. |
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Páginas: |
11 p. |
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Série: |
(Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 23). |
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Idioma: |
Português |
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Conteúdo: |
Utilizando frutos de pequi, coletados na região de Sete Lagoas - MG, em 1986, foi avaliada a presença de inibidores de germinação nas sementes e nas partes do fruto que a envolvem (polpa, espinhos e endocarpo). a extração foi a 5o C por 24 horas, utilizando dois solventes: água e metanol a 80%. Para testar a presença de inibidores, foram utilizadas sementes de alface (Lactuca sativa L.), cultivar repolhuda, com 88,5% de germinação. Os extratos aquoso e metanólico do endocarpo e dos espinhos e o extrato metanólico da polpa diminuíram, significativamente, tanto a velocidade como a porcentagem de germinação. O extrato aquoso da polpa não afetou o número de sementes germinadas, porém diminuiu a velocidade de germinação. Os extratos aquosos e metanólicos da semente não apresentaram qualquer efeito de inibição da germinação. |
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Palavras-Chave: |
elocity germination index; Germination index; Germination ratio; Propagação vegetal: Germinação; rowth regulators. |
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Thesagro: |
Caryocar Brasiliense; Cerrado; Fruto; Germinação; Pequi; Regulador de Crescimento; Semente. |
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Thesaurus NAL: |
growth regulators. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAC-2010/23731/1/bolpd-23.pdf
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Marc: |
LEADER 01712nam a2200301 a 4500
001 1567089
005 2013-02-28
008 2001 bl uuuu u0uu1 u #d
100 1 $aMELO, J. T. de
245 $aInibidores de germinacao em frutos e sementes de pequi.
260 $aPlanaltina: Embrapa Cerrados$c2001
300 $a11 p.
490 $a(Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 23).
520 $aUtilizando frutos de pequi, coletados na região de Sete Lagoas - MG, em 1986, foi avaliada a presença de inibidores de germinação nas sementes e nas partes do fruto que a envolvem (polpa, espinhos e endocarpo). a extração foi a 5o C por 24 horas, utilizando dois solventes: água e metanol a 80%. Para testar a presença de inibidores, foram utilizadas sementes de alface (Lactuca sativa L.), cultivar repolhuda, com 88,5% de germinação. Os extratos aquoso e metanólico do endocarpo e dos espinhos e o extrato metanólico da polpa diminuíram, significativamente, tanto a velocidade como a porcentagem de germinação. O extrato aquoso da polpa não afetou o número de sementes germinadas, porém diminuiu a velocidade de germinação. Os extratos aquosos e metanólicos da semente não apresentaram qualquer efeito de inibição da germinação.
650 $agrowth regulators
650 $aCaryocar Brasiliense
650 $aCerrado
650 $aFruto
650 $aGerminação
650 $aPequi
650 $aRegulador de Crescimento
650 $aSemente
653 $aelocity germination index
653 $aGermination index
653 $aGermination ratio
653 $aPropagação vegetal: Germinação
653 $arowth regulators
700 1 $aGONCALVES, A. N.
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Embrapa Cerrados (CPAC) |
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