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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
21/12/2017 |
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Data da última atualização: |
21/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
IVOMOTO, S. T.; SAKURAY, L. M.; FERREIRA, L. P.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; POT, D.; LEROY, T.; VIEIRA, L. G. E.; DOMINGUES, D. S.; PEREIRA, L. F. P. |
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Afiliação: |
Suzana T. Ivamoto, IAPAR/UEL; Leonardo M. Sakuray, IAPAR/UEL; Lucia P. Ferreira, IAPAR; Cíntia S. G. Kitzberger, IAPAR; Maria B. S. Scholz, IAPAR; David Pot, CIRAD; Thierry Leroy, CIRAD; Luiz G. E. Vieira, UNOEST; Douglas S. Domingues, UNESP; LUIZ FILIPE PROTASIO PEREIRA, SAPC. |
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Título: |
Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plant Physiology and Biochemistry, v. 111, p. 340-347, February, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30e240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways. MenosLipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30e240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warra... Mostrar Tudo |
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Palavras-Chave: |
Cafestol; Kahweol; RT-qPCR. |
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Thesagro: |
Café. |
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Thesaurus Nal: |
Gene expression; High performance liquid chromatography. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169675/1/Diterpenes-biochemical-profile.pdf
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Marc: |
LEADER 02581naa a2200301 a 4500
001 2083355
005 2017-12-21
008 2017 bl uuuu u00u1 u #d
100 1 $aIVOMOTO, S. T.
245 $aDiterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development.$h[electronic resource]
260 $c2017
520 $aLipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30e240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways.
650 $aGene expression
650 $aHigh performance liquid chromatography
650 $aCafé
653 $aCafestol
653 $aKahweol
653 $aRT-qPCR
700 1 $aSAKURAY, L. M.
700 1 $aFERREIRA, L. P.
700 1 $aKITZBERGER, C. S. G.
700 1 $aSCHOLZ, M. B. S.
700 1 $aPOT, D.
700 1 $aLEROY, T.
700 1 $aVIEIRA, L. G. E.
700 1 $aDOMINGUES, D. S.
700 1 $aPEREIRA, L. F. P.
773 $tPlant Physiology and Biochemistry$gv. 111, p. 340-347, February, 2017.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
01/04/2011 |
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Data da última atualização: |
19/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
CALIXTO JÚNIOR, J. T.; DRUMOND, M. A.; ALVES JÚNIOR, F. T. |
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Afiliação: |
JOÃO TAVARES CALIXTO JÚNIOR; MARCOS ANTONIO DRUMOND, CPATSA; FRANCISCO TARCÍSIO ALVES JÚNIOR. |
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Título: |
Estrutura e distribuição espacial de Mimosa tenuiflora (Willd.) Poir. em dois fragmentos de Caatinga em Pernambuco. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Revista Caatinga, Mossoró, v. 24, n. 2, p. 95-100, 2011. |
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Idioma: |
Português |
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Conteúdo: |
Este estudo objetivou investigar aspectos estruturais e ecológicos de sua população, com base em dados de diâmetro e altura, bem como caracterização do seu padrão de distribuição nas áreas estudadas. |
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Palavras-Chave: |
Estrutura de populações; Fitossociologia; Pernambuco; Semiárido; Vegetação caducifólia espinhosa. |
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Thesagro: |
Caatinga; Jurema Preta. |
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Thesaurus NAL: |
Mimosa tenuiflora. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31889/1/Drumond-2011.pdf
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Marc: |
LEADER 00974naa a2200241 a 4500
001 1884693
005 2023-09-19
008 2011 bl uuuu u00u1 u #d
100 1 $aCALIXTO JÚNIOR, J. T.
245 $aEstrutura e distribuição espacial de Mimosa tenuiflora (Willd.) Poir. em dois fragmentos de Caatinga em Pernambuco.$h[electronic resource]
260 $c2011
520 $aEste estudo objetivou investigar aspectos estruturais e ecológicos de sua população, com base em dados de diâmetro e altura, bem como caracterização do seu padrão de distribuição nas áreas estudadas.
650 $aMimosa tenuiflora
650 $aCaatinga
650 $aJurema Preta
653 $aEstrutura de populações
653 $aFitossociologia
653 $aPernambuco
653 $aSemiárido
653 $aVegetação caducifólia espinhosa
700 1 $aDRUMOND, M. A.
700 1 $aALVES JÚNIOR, F. T.
773 $tRevista Caatinga, Mossoró$gv. 24, n. 2, p. 95-100, 2011.
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