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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
12/06/2023 |
Data da última atualização: |
12/06/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RAMOS, C. P.; DORNELES, E. M. S.; HAAS, D. J.; VESCHI, J. L. A.; LOUREIRO, D.; PORTELA, R. D.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. |
Afiliação: |
CAROLINA PANTUZZA RAMOS, UFMG; ELAINE MARIA SELES DORNELES, UFLA; DIONEI JOAQUIM HAAS, UFMG; JOSIR LAINE APARECIDA VESCHI, CPATSA; DAN LOUREIRO, UFBA; RICARDO DIAS PORTELA, UFBA; VASCO AZEVEDO, UFMG; MARCOS BRYAN HEINEMANN, USP - São Paulo; ANDREY PEREIRA LAGE, UFMG. |
Título: |
Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Ciência Rural, v. 52, n. 11, e20210328, 2022. |
DOI: |
http://doi.org/10.1590/0103-8478cr20210328 |
Idioma: |
Inglês |
Conteúdo: |
The aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could be useful as an epidemiological tool to control caseous lymphadenitis. Moreover, our results also indicate the potential of ERIC 1+2-PCR for the genotyping of other species of Corynebacterium other than C. pseudotuberculosis. MenosThe aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could ... Mostrar Tudo |
Palavras-Chave: |
Epidemiologia molecular; ERIC 1+2-PCR; Genotipagem. |
Thesagro: |
Doença Animal; Linfadenite Caseosa. |
Thesaurus Nal: |
Caseous lymphadenitis; Genotyping; Molecular epidemiology. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154376/1/Molecular-characterization-of-Corynebacterium-pseudotuberculosis-2022.pdf
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Marc: |
LEADER 02705naa a2200325 a 4500 001 2154376 005 2023-06-12 008 2022 bl uuuu u00u1 u #d 024 7 $ahttp://doi.org/10.1590/0103-8478cr20210328$2DOI 100 1 $aRAMOS, C. P. 245 $aMolecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR.$h[electronic resource] 260 $c2022 520 $aThe aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could be useful as an epidemiological tool to control caseous lymphadenitis. Moreover, our results also indicate the potential of ERIC 1+2-PCR for the genotyping of other species of Corynebacterium other than C. pseudotuberculosis. 650 $aCaseous lymphadenitis 650 $aGenotyping 650 $aMolecular epidemiology 650 $aDoença Animal 650 $aLinfadenite Caseosa 653 $aEpidemiologia molecular 653 $aERIC 1+2-PCR 653 $aGenotipagem 700 1 $aDORNELES, E. M. S. 700 1 $aHAAS, D. J. 700 1 $aVESCHI, J. L. A. 700 1 $aLOUREIRO, D. 700 1 $aPORTELA, R. D. 700 1 $aAZEVEDO, V. 700 1 $aHEINEMANN, M. B. 700 1 $aLAGE, A. P. 773 $tCiência Rural$gv. 52, n. 11, e20210328, 2022.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
10/03/2004 |
Data da última atualização: |
05/06/2018 |
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
Autoria: |
FIGUEIREDO, J. E. F.; COELHO, V. T. da S.; DE PAOLI, H. C.; BRESSAN, W.; PURCINO, A. A. C.; ROCHA, T. L.; PRATES, M. V. |
Afiliação: |
JOSE EDSON FONTES FIGUEIREDO, CNPMS; ANTONIO ALVARO CORSETTI PURCINO, CNPMS. |
Título: |
Desenvolvimento de marcador molecular para Bacillus subtilis utilizando proteomics. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
Sete Lagoas: Embrapa Milho e Sorgo, 2003. |
Páginas: |
6 p. |
Série: |
(Embrapa Milho e Sorgo. Comunicado técnico, 67). |
Idioma: |
Português |
Thesagro: |
Marcador genético. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPMS/16148/1/Com_67.pdf
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Marc: |
LEADER 00650nam a2200205 a 4500 001 1487513 005 2018-06-05 008 2003 bl uuuu u0uu1 u #d 100 1 $aFIGUEIREDO, J. E. F. 245 $aDesenvolvimento de marcador molecular para Bacillus subtilis utilizando proteomics.$h[electronic resource] 260 $aSete Lagoas: Embrapa Milho e Sorgo$c2003 300 $a6 p. 490 $a(Embrapa Milho e Sorgo. Comunicado técnico, 67). 650 $aMarcador genético 700 1 $aCOELHO, V. T. da S. 700 1 $aDE PAOLI, H. C. 700 1 $aBRESSAN, W. 700 1 $aPURCINO, A. A. C. 700 1 $aROCHA, T. L. 700 1 $aPRATES, M. V.
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