Data/hora: 05/06/2024 12:18:02

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	24/12/2016	
Data da última atualização: 	07/02/2017	
Autoria: 	MIRANDA, Í. K. S. P. B.; MIRANDA, A. F. S.; SOUZA, F. V. D.; PIROVANI, C. P.; PEPE, I. M.; RODOWANSKI, I. J.; FERREIRA, K. T. de S. E.; VAZ, L. M. S.; ASSIS, S. A. de.	
Afiliação: 	ÍNGARA KEISLE SÃO PAULO BARRETTO MIRANDA; ANDERSON FONTES SUZART MIRANDA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; CARLOS PRIMINHO PIROVANI; IURI MUNIZ PEPE; IVANOÉ JOÃO RODOWANSKI; KATIÚCIA TÍCILA DE SOUZA EDUVIRGENS FERREIRA; LUCIANO MENDES SOUZA VAZ; SANDRA APARECIDA DE ASSIS.	
Título: 	The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells.	
Ano de publicação: 	2016	
Fonte/Imprenta: 	International Journal of Food Sciences and Nutrition, 2016	
ISSN: 	0963-7486	
Idioma: 	Português	
Conteúdo: 	The current study aims to extract bromelain from different parts (stem, crown, peels, pulp and leaves) of Ananas comosus var. comosus AGB 772; to determine of optimum pH and temperature; to test bromelain stability in disodium EDTA and sodium benzoate, and to investigate its pharmacological activity on B16F10 murine melanoma cells in vitro. The highest enzymatic activity was found in bromelain extracted from the pulp and peel. The optimum bromelain pH among all studied pineapple parts was 6.0. The optimum temperature was above 50?°C in all bromelain extracts. The fluorescence analysis confirmed the stability of bromelain in the presence of EDTA and sodium benzoate. Bromelain was pharmacologically active against B16F10 melanoma cells and it was possible verifying approximately 100% inhibition of tumor cell proliferation in vitro. Since bromelain activity was found in different parts of pineapple plants, pineapple residues from the food industry may be used for bromelain extraction.	
Thesagro: 	Bromélia.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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