| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Amazônia Oriental. |
|
Data corrente: |
29/03/1996 |
|
Data da última atualização: |
03/10/2012 |
|
Autoria: |
PINTO, G. P. |
|
Título: |
Contribuicao ao estudo tecnologico e economico da neutralizacao do oleo de babacu. |
|
Ano de publicação: |
1952 |
|
Fonte/Imprenta: |
Belem: [s.n.], 1952. |
|
Páginas: |
38p. |
|
Descrição Física: |
il. |
|
Idioma: |
Português |
|
Notas: |
Tese Livre-Docencia. |
|
Conteúdo: |
Versa sobre o estudo tecnico e economico da neutralizacao do oleo de babacu, principal materia gorda manufaturada pelas industrias do norte do pais. Nos primeiros capitulos sintetiza ao maximo, as nocoes gerais sobre os inconvenientes apresentados pela acidez livre, por vezes elevada, dos nossos oleos de babacu e outros; sua formacao e influencia nos fenomenos da oxidacao e rancificacao das materias graxas em geral, originando-se dai o interesse fundamental da industria, em retirar a acidez, beneficiando enfim o produto bruto, a fim de melhorar suas condicoes de estabilidade frente aos agentes oxidantes naturais, como tambem aumentar suas qualidades alimenticias. Logo depois, foram descritas as caracteristicas do cocos da palmeira babacu (Orbignya Martiana Barb. Roa.) provenientes do Estado do Para, confrontando-as com as dos cocos procedentes do Maranhao, principal produtor. Mostramos a acidez media relativamente elevada que o oleo bruto apresenta, e a quanto se eleva a producao nacional. |
|
Palavras-Chave: |
Analysis; Babassu; Economy; Oil; Processing; Production. |
|
Thesagro: |
Acidez; Análise; Babaçu; Economia; Óleo Vegetal; Orbignya Martiana; Processamento; Produção; Tecnologia. |
|
Thesaurus Nal: |
acidity. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01786nam a2200325 a 4500
001 1401386
005 2012-10-03
008 1952 bl uuuu m 00u1 u #d
100 1 $aPINTO, G. P.
245 $aContribuicao ao estudo tecnologico e economico da neutralizacao do oleo de babacu.
260 $aBelem: [s.n.]$c1952
300 $a38p.$cil.
500 $aTese Livre-Docencia.
520 $aVersa sobre o estudo tecnico e economico da neutralizacao do oleo de babacu, principal materia gorda manufaturada pelas industrias do norte do pais. Nos primeiros capitulos sintetiza ao maximo, as nocoes gerais sobre os inconvenientes apresentados pela acidez livre, por vezes elevada, dos nossos oleos de babacu e outros; sua formacao e influencia nos fenomenos da oxidacao e rancificacao das materias graxas em geral, originando-se dai o interesse fundamental da industria, em retirar a acidez, beneficiando enfim o produto bruto, a fim de melhorar suas condicoes de estabilidade frente aos agentes oxidantes naturais, como tambem aumentar suas qualidades alimenticias. Logo depois, foram descritas as caracteristicas do cocos da palmeira babacu (Orbignya Martiana Barb. Roa.) provenientes do Estado do Para, confrontando-as com as dos cocos procedentes do Maranhao, principal produtor. Mostramos a acidez media relativamente elevada que o oleo bruto apresenta, e a quanto se eleva a producao nacional.
650 $aacidity
650 $aAcidez
650 $aAnálise
650 $aBabaçu
650 $aEconomia
650 $aÓleo Vegetal
650 $aOrbignya Martiana
650 $aProcessamento
650 $aProdução
650 $aTecnologia
653 $aAnalysis
653 $aBabassu
653 $aEconomy
653 $aOil
653 $aProcessing
653 $aProduction
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Amazônia Oriental (CPATU) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
26/12/2018 |
|
Data da última atualização: |
24/01/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
SOUZA, G. T. de; HELL, R. C. R.; SOUZA, J. F. da S.; CAMARGO, L. S. de A. |
|
Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
|
Título: |
Easy in vitro synthesis of optimised functioning reporter mRNA from common eGFP plasmid. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Molecular Biotechnology, v. 60, n. 10, p. 762-771, 2018. |
|
DOI: |
10.1007/s12033-018-0112-5 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract The extensive growth in number and importance of experiments and clinical-aimed techniques based solely or majorly on the activity of RNA strands, e.g. CRSPR/Cas9 and siRNA, has put emphasis on the necessity of standardisation of experiments with RNA. Considering RNA degradation during its handling seems to be a major hindrance in all RNA-based tools, the assessment of its integrity is of utmost importance. Furthermore, evaluating whether the RNA to be transfected is intact requires time-consuming electrophoresis protocol. In view of the RNA lability and the necessity for controlling experiments performed with this molecule, the transfection of a reporter mRNA may be of aid in optimising experiments. Nevertheless, commercial reporter mRNAs are far less available than plasmids for such purpose. Thus, in this work, we aimed at the optimisation of an easily performed protocol to produce a suitable eGFP mRNA. By utilising molecular biology kits customarily employed in molecular biology laboratories working with RNA-based techniques and starting from any eGFP coding vector, we produced four mRNA molecules: (1) eGFP mRNA (non-polyadenylated); (2) Kozak-eGFP mRNA (non-polyadenylated, produced from the Kozak-containing amplicon); (3) eGFP-PolyA mRNA (polyadenylated); (4) Kozak-eGFP-PolyA mRNA (containing both signals, Kozak sequence and poly(A) tail). These mRNA molecules were transfected into HEK 293 FT cells, readily transfectable, and into the MDBK bovine lineage, which has been observed as difficult-to-transfect DNA constructs. eGFP expression could be detected both by flow cytometry and by fluorescence microscopy after transfection with the polyadenylated mRNAs. Upon cytometric analysis, we noted a marked difference among the mRNA groups (p < 0.01), both in fluorescent population percentage and in florescence intensity. We showed here the necessity of the polyadenylation step in order to achieve cell expression of the eGFP observable under fluorescence microscopy. The presence of the Kozak sequence, as a 5' element, seems to augment significantly the level of protein produced upon mRNA transfection. We presented here an easy protocol to allow production of functioning mRNAs from any DNA construct. The molecules produced may aid in the standardisation and controlling most of the RNA-related experiments as well as it gives proper guidance for researchers performing expression of other proteins through mRNA transfection. MenosAbstract The extensive growth in number and importance of experiments and clinical-aimed techniques based solely or majorly on the activity of RNA strands, e.g. CRSPR/Cas9 and siRNA, has put emphasis on the necessity of standardisation of experiments with RNA. Considering RNA degradation during its handling seems to be a major hindrance in all RNA-based tools, the assessment of its integrity is of utmost importance. Furthermore, evaluating whether the RNA to be transfected is intact requires time-consuming electrophoresis protocol. In view of the RNA lability and the necessity for controlling experiments performed with this molecule, the transfection of a reporter mRNA may be of aid in optimising experiments. Nevertheless, commercial reporter mRNAs are far less available than plasmids for such purpose. Thus, in this work, we aimed at the optimisation of an easily performed protocol to produce a suitable eGFP mRNA. By utilising molecular biology kits customarily employed in molecular biology laboratories working with RNA-based techniques and starting from any eGFP coding vector, we produced four mRNA molecules: (1) eGFP mRNA (non-polyadenylated); (2) Kozak-eGFP mRNA (non-polyadenylated, produced from the Kozak-containing amplicon); (3) eGFP-PolyA mRNA (polyadenylated); (4) Kozak-eGFP-PolyA mRNA (containing both signals, Kozak sequence and poly(A) tail). These mRNA molecules were transfected into HEK 293 FT cells, readily transfectable, and into the MDBK bovine lineage, which ... Mostrar Tudo |
|
Palavras-Chave: |
CRISPR/Cas9; EGFP; GFP; Kozak; MRNA; Sequence. |
|
Thesagro: |
RNA. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 03181naa a2200253 a 4500
001 2102521
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1007/s12033-018-0112-5$2DOI
100 1 $aSOUZA, G. T. de
245 $aEasy in vitro synthesis of optimised functioning reporter mRNA from common eGFP plasmid.$h[electronic resource]
260 $c2018
520 $aAbstract The extensive growth in number and importance of experiments and clinical-aimed techniques based solely or majorly on the activity of RNA strands, e.g. CRSPR/Cas9 and siRNA, has put emphasis on the necessity of standardisation of experiments with RNA. Considering RNA degradation during its handling seems to be a major hindrance in all RNA-based tools, the assessment of its integrity is of utmost importance. Furthermore, evaluating whether the RNA to be transfected is intact requires time-consuming electrophoresis protocol. In view of the RNA lability and the necessity for controlling experiments performed with this molecule, the transfection of a reporter mRNA may be of aid in optimising experiments. Nevertheless, commercial reporter mRNAs are far less available than plasmids for such purpose. Thus, in this work, we aimed at the optimisation of an easily performed protocol to produce a suitable eGFP mRNA. By utilising molecular biology kits customarily employed in molecular biology laboratories working with RNA-based techniques and starting from any eGFP coding vector, we produced four mRNA molecules: (1) eGFP mRNA (non-polyadenylated); (2) Kozak-eGFP mRNA (non-polyadenylated, produced from the Kozak-containing amplicon); (3) eGFP-PolyA mRNA (polyadenylated); (4) Kozak-eGFP-PolyA mRNA (containing both signals, Kozak sequence and poly(A) tail). These mRNA molecules were transfected into HEK 293 FT cells, readily transfectable, and into the MDBK bovine lineage, which has been observed as difficult-to-transfect DNA constructs. eGFP expression could be detected both by flow cytometry and by fluorescence microscopy after transfection with the polyadenylated mRNAs. Upon cytometric analysis, we noted a marked difference among the mRNA groups (p < 0.01), both in fluorescent population percentage and in florescence intensity. We showed here the necessity of the polyadenylation step in order to achieve cell expression of the eGFP observable under fluorescence microscopy. The presence of the Kozak sequence, as a 5' element, seems to augment significantly the level of protein produced upon mRNA transfection. We presented here an easy protocol to allow production of functioning mRNAs from any DNA construct. The molecules produced may aid in the standardisation and controlling most of the RNA-related experiments as well as it gives proper guidance for researchers performing expression of other proteins through mRNA transfection.
650 $aRNA
653 $aCRISPR/Cas9
653 $aEGFP
653 $aGFP
653 $aKozak
653 $aMRNA
653 $aSequence
700 1 $aHELL, R. C. R.
700 1 $aSOUZA, J. F. da S.
700 1 $aCAMARGO, L. S. de A.
773 $tMolecular Biotechnology$gv. 60, n. 10, p. 762-771, 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
