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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
21/03/1998 |
Data da última atualização: |
21/03/1998 |
Autoria: |
PIFFER, I. A.; ROSS, R. F. |
Afiliação: |
CNPSA. |
Título: |
Immunofluorescente technique for detection of mycoplasma hyopneumoniae in swine lungs. |
Ano de publicação: |
1985 |
Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v.20, n.8, p.877-882, ago. 1985. |
Idioma: |
Inglês |
Conteúdo: |
Direct and indirect immunofluorescence techniques and a culture method were compared for detection of Mycoplasma hyopneumoniae in lungs of experimentally infected swine. Pneumonic lungs were obtained from SPF pigs that had been exposed by contact 6 to 7 weeks previously to other SPF pigs which had been inoculated with the organism. A good association was found between isolation of M. hyopneumoniae, positive immunofluorescence test results and presence of macroscopic or microscopic lesions typical of my coplasmal pneumonia of swine. Direct and indirect immunofluorescence tests were equally efficient for detection of M. hyopneumoniae in sections of lung. An azo dye counterstain reduced nonspecific fluorescence and provided a good color contrast without reducing sensitivity of the indirect immunofluorescence test. |
Palavras-Chave: |
Diagnosis; Enzootic pneumonia; Mycoplasmal pneumonia; Pneumonia micoplasmica. |
Thesagro: |
Diagnostico; Pneumonia Enzoótica. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01440naa a2200205 a 4500 001 1104578 005 1998-03-21 008 1985 bl uuuu u00u1 u #d 100 1 $aPIFFER, I. A. 245 $aImmunofluorescente technique for detection of mycoplasma hyopneumoniae in swine lungs. 260 $c1985 520 $aDirect and indirect immunofluorescence techniques and a culture method were compared for detection of Mycoplasma hyopneumoniae in lungs of experimentally infected swine. Pneumonic lungs were obtained from SPF pigs that had been exposed by contact 6 to 7 weeks previously to other SPF pigs which had been inoculated with the organism. A good association was found between isolation of M. hyopneumoniae, positive immunofluorescence test results and presence of macroscopic or microscopic lesions typical of my coplasmal pneumonia of swine. Direct and indirect immunofluorescence tests were equally efficient for detection of M. hyopneumoniae in sections of lung. An azo dye counterstain reduced nonspecific fluorescence and provided a good color contrast without reducing sensitivity of the indirect immunofluorescence test. 650 $aDiagnostico 650 $aPneumonia Enzoótica 653 $aDiagnosis 653 $aEnzootic pneumonia 653 $aMycoplasmal pneumonia 653 $aPneumonia micoplasmica 700 1 $aROSS, R. F. 773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv.20, n.8, p.877-882, ago. 1985.
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