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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
08/10/2008 |
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Data da última atualização: |
08/10/2008 |
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Autoria: |
PI?L, V.; SCHLAGHAMERSKÝ, J.; TRÍSKA, J. |
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Título: |
The effects of polycyclic aromatic hydrocarbons on terrestrial annelids in urban soils. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
The impact of soil pollution on terrestrial annelids was investigated in two public parks in the city
of Brno (Czech Republic). One of the major group of pollutants emitted by motor vehicles are
polycyclic aromatic hydrocarbons (PAHs). The soil fauna of urban greens is therefore particularly
exposed to these pollutants. The objective of our study was to assess the contents of PAHs in
soil and their effects on the communities of enchytraeids and earthworms. In addition, PAH
concentrations in soil were compared with those in animal tissues. Two lawns, both adjacent to
a street with high traffic intensity, were selected as sampling sites. Samples were taken in 1 m,
5 m and 30 m distance from the street in spring and autumn 2007 (two transects per lawn and
sampling date). Each sampling point included three or four adjacent soil cores for enchytraeid
extraction (depending on soil moisture and thus enchytraeid abundance) and one earthworm
sampling point using the electrical octet method. Enchytraeids were extracted from the cores by
the wet funnel method (without heating). The obtained specimens were counted and identified.
A part of the obtained specimens was used for PAH analysis. These specimens were depurated,
freeze-dried, weighted and processed for atomic absorption spectrophotometry. The contents
of 16 PAHs were determined. Further, two sets of soil samples were taken to analyse basic
chemical parameters and PAH contents, respectively.
The content of PAHs in soil ranged from 3.2 to 17.65 mg/kg and decreased with the distance
from the street (ANOVA, F = 3.98, p = 0.05). Four species of earthworms were recorded, among
which the anecic Lumbricus terrestris and the endogeic Aporrectodea caliginosa highly
predominated. Earthworm density tended to increase from the street edge towards the centre
of the park, with mean values of 114, 186 and 218 ind./m2 in the distance of 1, 5 and 30 m,
respectively. However, the differences were insignificant (ANOVA, F = 0.79, p = 0.48). The
enchytraeid community was dominated by Henlea ventriculosa, Enchytraeus buchholzi s.l., three
Achaeta species and Fridericia spp., particularly F. hegemon. Enchytraeid densities in spring
were very low, with mean values of 4010, 1620 and 1580 ind./m2 in the distance of 1, 5 and 30
m respectively. The corresponding values for the autumn sampling were 4780, 6210 and 4260
ind./m2. The differences were not significant and there was by no means a trend of densities
increasing with the distance to the streets. Earthworm tissue concentrations of PAHs were low,
not higher than 0.78 mg/kg. There were no significant correlations, neither between soil and
tissue contents of PAHs nor between these parameters and earthworm density. Earthworm
density was, however, negatively correlated with soil pH. Chemical analyses are still on-going at
present (March 2008).
The study was supported by the Grant Agency of the AS CR, project No. IAA600660608, by the
Czech Ministry of Education, Youth and Sports, Research Plan No. MSM0021622416, and by
the Academy of Sciences of the Czech Republic, Research Plan No. AV0Z60660521. MenosThe impact of soil pollution on terrestrial annelids was investigated in two public parks in the city
of Brno (Czech Republic). One of the major group of pollutants emitted by motor vehicles are
polycyclic aromatic hydrocarbons (PAHs). The soil fauna of urban greens is therefore particularly
exposed to these pollutants. The objective of our study was to assess the contents of PAHs in
soil and their effects on the communities of enchytraeids and earthworms. In addition, PAH
concentrations in soil were compared with those in animal tissues. Two lawns, both adjacent to
a street with high traffic intensity, were selected as sampling sites. Samples were taken in 1 m,
5 m and 30 m distance from the street in spring and autumn 2007 (two transects per lawn and
sampling date). Each sampling point included three or four adjacent soil cores for enchytraeid
extraction (depending on soil moisture and thus enchytraeid abundance) and one earthworm
sampling point using the electrical octet method. Enchytraeids were extracted from the cores by
the wet funnel method (without heating). The obtained specimens were counted and identified.
A part of the obtained specimens was used for PAH analysis. These specimens were depurated,
freeze-dried, weighted and processed for atomic absorption spectrophotometry. The contents
of 16 PAHs were determined. Further, two sets of soil samples were taken to analyse basic
chemical parameters and PAH contents, respectively.
The content of PAHs in soil ranged fro... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 03806naa a2200145 a 4500
001 1315096
005 2008-10-08
008 2008 bl uuuu u00u1 u #d
100 1 $aPI?L, V.
245 $aThe effects of polycyclic aromatic hydrocarbons on terrestrial annelids in urban soils.
260 $c2008
520 $aThe impact of soil pollution on terrestrial annelids was investigated in two public parks in the city of Brno (Czech Republic). One of the major group of pollutants emitted by motor vehicles are polycyclic aromatic hydrocarbons (PAHs). The soil fauna of urban greens is therefore particularly exposed to these pollutants. The objective of our study was to assess the contents of PAHs in soil and their effects on the communities of enchytraeids and earthworms. In addition, PAH concentrations in soil were compared with those in animal tissues. Two lawns, both adjacent to a street with high traffic intensity, were selected as sampling sites. Samples were taken in 1 m, 5 m and 30 m distance from the street in spring and autumn 2007 (two transects per lawn and sampling date). Each sampling point included three or four adjacent soil cores for enchytraeid extraction (depending on soil moisture and thus enchytraeid abundance) and one earthworm sampling point using the electrical octet method. Enchytraeids were extracted from the cores by the wet funnel method (without heating). The obtained specimens were counted and identified. A part of the obtained specimens was used for PAH analysis. These specimens were depurated, freeze-dried, weighted and processed for atomic absorption spectrophotometry. The contents of 16 PAHs were determined. Further, two sets of soil samples were taken to analyse basic chemical parameters and PAH contents, respectively. The content of PAHs in soil ranged from 3.2 to 17.65 mg/kg and decreased with the distance from the street (ANOVA, F = 3.98, p = 0.05). Four species of earthworms were recorded, among which the anecic Lumbricus terrestris and the endogeic Aporrectodea caliginosa highly predominated. Earthworm density tended to increase from the street edge towards the centre of the park, with mean values of 114, 186 and 218 ind./m2 in the distance of 1, 5 and 30 m, respectively. However, the differences were insignificant (ANOVA, F = 0.79, p = 0.48). The enchytraeid community was dominated by Henlea ventriculosa, Enchytraeus buchholzi s.l., three Achaeta species and Fridericia spp., particularly F. hegemon. Enchytraeid densities in spring were very low, with mean values of 4010, 1620 and 1580 ind./m2 in the distance of 1, 5 and 30 m respectively. The corresponding values for the autumn sampling were 4780, 6210 and 4260 ind./m2. The differences were not significant and there was by no means a trend of densities increasing with the distance to the streets. Earthworm tissue concentrations of PAHs were low, not higher than 0.78 mg/kg. There were no significant correlations, neither between soil and tissue contents of PAHs nor between these parameters and earthworm density. Earthworm density was, however, negatively correlated with soil pH. Chemical analyses are still on-going at present (March 2008). The study was supported by the Grant Agency of the AS CR, project No. IAA600660608, by the Czech Ministry of Education, Youth and Sports, Research Plan No. MSM0021622416, and by the Academy of Sciences of the Czech Republic, Research Plan No. AV0Z60660521.
700 1 $aSCHLAGHAMERSKÝ, J.
700 1 $aTRÍSKA, J.
773 $tIn: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
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Registro original: |
Embrapa Florestas (CNPF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
01/09/2014 |
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Data da última atualização: |
09/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MÜLLER, B. S. de F.; SAKAMOTO, T.; MENEZES, I. P. P. de; PRADO, G. S.; MARTINS, W. S.; BRONDANI, C.; BARROS, E. G. de; VIANELLO, R. P. |
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Afiliação: |
BÁRBARA SALOMÃO DE FARIA MÜLLER, UFV; TETSU SAKAMOTO, UFMG; IVANDILSON PESSOA PINTO DE MENEZES, INSTITUTO FEDERAL GOIANO, Urutaí-GO; GUILHERME SOUZA PRADO, bolsista CNPAF; WELLINGTON SANTOS MARTINS, UFG; CLAUDIO BRONDANI, CNPAF; EVERALDO GONÇALVES DE BARROS, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
Analysis of BAC-end sequences in common bean (Phaseolus vulgaris L.) towards the development and characterization of long motifs SSRs. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Molecular Biology, Dordrecht, v. 86, n. 4/5, p. 455-470, Nov. 2014. |
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DOI: |
10.1007/s11103-014-0240-7 |
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Idioma: |
Inglês |
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Conteúdo: |
The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (≥trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E ≥ 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides motifs and bringing together relevant genetic characteristics useful for breeding programs. Additionally, the BESs were incorporated into the international genome-sequencing project for the common bean. MenosThe increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (≥trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E ≥ 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides mo... Mostrar Tudo |
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Thesagro: |
Feijão; Genética molecular; Phaseolus vulgaris. |
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Thesaurus NAL: |
Beans; Fabaceae; Genomics; Microsatellite repeats; Molecular genetics; Transcription factors; Transposons. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02734naa a2200337 a 4500
001 1993888
005 2014-12-09
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s11103-014-0240-7$2DOI
100 1 $aMÜLLER, B. S. de F.
245 $aAnalysis of BAC-end sequences in common bean (Phaseolus vulgaris L.) towards the development and characterization of long motifs SSRs.$h[electronic resource]
260 $c2014
520 $aThe increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (≥trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E ≥ 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides motifs and bringing together relevant genetic characteristics useful for breeding programs. Additionally, the BESs were incorporated into the international genome-sequencing project for the common bean.
650 $aBeans
650 $aFabaceae
650 $aGenomics
650 $aMicrosatellite repeats
650 $aMolecular genetics
650 $aTranscription factors
650 $aTransposons
650 $aFeijão
650 $aGenética molecular
650 $aPhaseolus vulgaris
700 1 $aSAKAMOTO, T.
700 1 $aMENEZES, I. P. P. de
700 1 $aPRADO, G. S.
700 1 $aMARTINS, W. S.
700 1 $aBRONDANI, C.
700 1 $aBARROS, E. G. de
700 1 $aVIANELLO, R. P.
773 $tPlant Molecular Biology, Dordrecht$gv. 86, n. 4/5, p. 455-470, Nov. 2014.
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