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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
16/06/2010 |
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Data da última atualização: |
02/09/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, M. B.; NASCIMENTO, L. B.; JUNIOR, M. L.; PETROFEZA, S. |
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Afiliação: |
M. B. OLIVEIRA, UNIVERSIDADE FEDERAL DE GOIÁS; L. B. NASCIMENTO, UNIVERSIDADE FEDERAL DE GOIÁS; MURILLO LOBO JUNIOR, CNPAF; S. PETROFEZA, UNIVERSIDADE FEDERAL DE GOIÁS. |
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Título: |
Characterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 9, n. 2, p. 994-1004, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Polygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be moderately induced by mechanical wounding. These results provide evidence that endopolygalacturonases contribute to the infection process during host colonization by promoting the release of plant cell oligogalacturonides, which are powerful signaling molecules and may also activate plant defenses, such as polygalacturonase-inhibiting proteins. MenosPolygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be... Mostrar Tudo |
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Palavras-Chave: |
Endopolygalacturonase; Polygalacturonase-inhibiting protein. |
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Thesagro: |
Doença fúngica; Feijão; Mofo branco; Phaseolus vulgaris; Sclerotinia sclerotiorum. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/18863/1/gmr776.pdf
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Marc: |
LEADER 02653naa a2200241 a 4500
001 1855158
005 2010-09-02
008 2010 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, M. B.
245 $aCharacterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection.$h[electronic resource]
260 $c2010
520 $aPolygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be moderately induced by mechanical wounding. These results provide evidence that endopolygalacturonases contribute to the infection process during host colonization by promoting the release of plant cell oligogalacturonides, which are powerful signaling molecules and may also activate plant defenses, such as polygalacturonase-inhibiting proteins.
650 $aDoença fúngica
650 $aFeijão
650 $aMofo branco
650 $aPhaseolus vulgaris
650 $aSclerotinia sclerotiorum
653 $aEndopolygalacturonase
653 $aPolygalacturonase-inhibiting protein
700 1 $aNASCIMENTO, L. B.
700 1 $aJUNIOR, M. L.
700 1 $aPETROFEZA, S.
773 $tGenetics and Molecular Research$gv. 9, n. 2, p. 994-1004, 2010.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
04/10/2022 |
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Data da última atualização: |
13/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SILVA, J. K. B. da; ARAUJO, G. G. L. de; SANTOS, E. M.; OLIVEIRA, J. S. de; CAMPOS, F. S.; GOIS, G. C.; SILVA, T. S.; MATIAS, A. G. da S.; RIBEIRO, O. L.; PERAZZO, A. F.; ZANINE, A. de M. |
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Afiliação: |
JUSCELINO KUBITSCHEK BEVENUTO DA SILVA, UFPB; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; EDSON MAURO SANTOS, UFPB; JULIANA SILVA DE OLIVEIRA, UFPB; FLEMING SENA CAMPOS, Bolsista CNPq; GLAYCIANE COSTA GOIS, UNIVASF; TIAGO SANTOS SILVA, IF Sertão Pernambuco; ALEX GOMES DA SILVA MATIAS, UNIVASF; OSSIVAL LOLATO RIBEIRO, UFRB; ALEXANDRE FERNANDES PERAZZO, UFMA; ANDERSON DE MOURA ZANINE, UFMA. |
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Título: |
Rendimiento de corderos alimentados con ensilaje como alimento total a base de nopal. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Revista Mexicana de Ciencias Pecuarias, v. 13, n. 1, p. 19-31, 2022. |
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DOI: |
https://doi.org/10.22319/rmcp.v13i1.5849 |
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Idioma: |
Espanhol |
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Conteúdo: |
Este estudio tuvo como objetivo evaluar la ingesta, digestibilidad aparente, balance hídrico, balance de nitrógeno y rendimiento productivo en corderos alimentados con ensilaje de nopal asociado con forrajes tropicales. En el experimento se utilizaron 40 corderos machos cruzados intactos, con un peso corporal inicial de 18.85 ± 1.2 kg. El diseño experimental fue completamente al azar, con 5 tratamientos y 8 repeticiones. Los tratamientos consistieron en dietas con diferentes proporciones de pasto buffel y salvado de trigo como fuentes de fibra detergente neutro (FDN), en ensilaje de alimento total (EAT) a base de nopal: EAT1 - 279 g/kg de pasto buffel; EAT2 - 240 g/kg de pasto buffel y 17 g/kg de salvado de trigo; EAT3 - 198 g/kg de pasto buffel y 34 g/kg de salvado de trigo; EAT4 - 108 g/kg de pasto buffel y 74 g/kg de salvado de trigo; EAT5 ? 118 g/kg de salvado de trigo. La ingesta más baja de FDN se encontró en corderos que consumieron EAT5 (402 g/día). La digestibilidad aparente de los carbohidratos no fibrosos fue mayor para EAT5, mientras que la digestibilidad aparente de FDN de EAT5 y EAT4 fue mayor que la de EAT1. Las dietas promovieron una ganancia diaria de peso promedio de 180.8 g/día. En condiciones experimentales, el uso de nopal forrajero y concentrado en forma de raciones mixtas totales de ensilaje conduce a una mayor ingesta de proteína cruda, carbohidratos no fibrosos, extracto etéreo y mayor digestibilidad de carbohidratos no fibrosos y fibra detergente neutro, sin embargo, todas las dietas fueron viables en la alimentación de ovinos confinados, proporcionando ganancias de hasta 198 g/d según la formulación de la dieta. MenosEste estudio tuvo como objetivo evaluar la ingesta, digestibilidad aparente, balance hídrico, balance de nitrógeno y rendimiento productivo en corderos alimentados con ensilaje de nopal asociado con forrajes tropicales. En el experimento se utilizaron 40 corderos machos cruzados intactos, con un peso corporal inicial de 18.85 ± 1.2 kg. El diseño experimental fue completamente al azar, con 5 tratamientos y 8 repeticiones. Los tratamientos consistieron en dietas con diferentes proporciones de pasto buffel y salvado de trigo como fuentes de fibra detergente neutro (FDN), en ensilaje de alimento total (EAT) a base de nopal: EAT1 - 279 g/kg de pasto buffel; EAT2 - 240 g/kg de pasto buffel y 17 g/kg de salvado de trigo; EAT3 - 198 g/kg de pasto buffel y 34 g/kg de salvado de trigo; EAT4 - 108 g/kg de pasto buffel y 74 g/kg de salvado de trigo; EAT5 ? 118 g/kg de salvado de trigo. La ingesta más baja de FDN se encontró en corderos que consumieron EAT5 (402 g/día). La digestibilidad aparente de los carbohidratos no fibrosos fue mayor para EAT5, mientras que la digestibilidad aparente de FDN de EAT5 y EAT4 fue mayor que la de EAT1. Las dietas promovieron una ganancia diaria de peso promedio de 180.8 g/día. En condiciones experimentales, el uso de nopal forrajero y concentrado en forma de raciones mixtas totales de ensilaje conduce a una mayor ingesta de proteína cruda, carbohidratos no fibrosos, extracto etéreo y mayor digestibilidad de carbohidratos no fibrosos y fibra detergente ne... Mostrar Tudo |
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Palavras-Chave: |
Conservação de alimentos; Equilíbrio hídrico. |
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Thesagro: |
Ganho de Peso; Ingestão; Nutrição Animal; Palma Forrageira; Silagem. |
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Thesaurus NAL: |
Opuntia ficus-indica; Silage. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1147109/1/Rendimiento-de-corderos-alimentados-con-ensilaje-como-alimento-total-a-base-de-nopal.-2022.pdf
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Marc: |
LEADER 02752naa a2200361 a 4500
001 2147109
005 2023-01-13
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.22319/rmcp.v13i1.5849$2DOI
100 1 $aSILVA, J. K. B. da
245 $aRendimiento de corderos alimentados con ensilaje como alimento total a base de nopal.$h[electronic resource]
260 $c2022
520 $aEste estudio tuvo como objetivo evaluar la ingesta, digestibilidad aparente, balance hídrico, balance de nitrógeno y rendimiento productivo en corderos alimentados con ensilaje de nopal asociado con forrajes tropicales. En el experimento se utilizaron 40 corderos machos cruzados intactos, con un peso corporal inicial de 18.85 ± 1.2 kg. El diseño experimental fue completamente al azar, con 5 tratamientos y 8 repeticiones. Los tratamientos consistieron en dietas con diferentes proporciones de pasto buffel y salvado de trigo como fuentes de fibra detergente neutro (FDN), en ensilaje de alimento total (EAT) a base de nopal: EAT1 - 279 g/kg de pasto buffel; EAT2 - 240 g/kg de pasto buffel y 17 g/kg de salvado de trigo; EAT3 - 198 g/kg de pasto buffel y 34 g/kg de salvado de trigo; EAT4 - 108 g/kg de pasto buffel y 74 g/kg de salvado de trigo; EAT5 ? 118 g/kg de salvado de trigo. La ingesta más baja de FDN se encontró en corderos que consumieron EAT5 (402 g/día). La digestibilidad aparente de los carbohidratos no fibrosos fue mayor para EAT5, mientras que la digestibilidad aparente de FDN de EAT5 y EAT4 fue mayor que la de EAT1. Las dietas promovieron una ganancia diaria de peso promedio de 180.8 g/día. En condiciones experimentales, el uso de nopal forrajero y concentrado en forma de raciones mixtas totales de ensilaje conduce a una mayor ingesta de proteína cruda, carbohidratos no fibrosos, extracto etéreo y mayor digestibilidad de carbohidratos no fibrosos y fibra detergente neutro, sin embargo, todas las dietas fueron viables en la alimentación de ovinos confinados, proporcionando ganancias de hasta 198 g/d según la formulación de la dieta.
650 $aOpuntia ficus-indica
650 $aSilage
650 $aGanho de Peso
650 $aIngestão
650 $aNutrição Animal
650 $aPalma Forrageira
650 $aSilagem
653 $aConservação de alimentos
653 $aEquilíbrio hídrico
700 1 $aARAUJO, G. G. L. de
700 1 $aSANTOS, E. M.
700 1 $aOLIVEIRA, J. S. de
700 1 $aCAMPOS, F. S.
700 1 $aGOIS, G. C.
700 1 $aSILVA, T. S.
700 1 $aMATIAS, A. G. da S.
700 1 $aRIBEIRO, O. L.
700 1 $aPERAZZO, A. F.
700 1 $aZANINE, A. de M.
773 $tRevista Mexicana de Ciencias Pecuarias$gv. 13, n. 1, p. 19-31, 2022.
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