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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
20/02/2013 |
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Data da última atualização: |
21/08/2017 |
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Autoria: |
PERSEGUINI, J. M. K. C; ROMÃO, L. R. de C.; BRIÑEZ, B.; SCALOPPI JÚNIOR, E. J.; GONÇALVES, P. de S.; BENCHIMOL, L. L. |
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Afiliação: |
JULIANA MORINI KÜPPER CARDOSO PERSEGUINI, UNICAMP; LINEU ROBERTO de CASTRO ROMÃO, UNICAMP; BORIS BRIÑEZ, UNICAMP; ERIVALDO JOSÉ SCALOPPI JÚNIOR, APTA; PAULO de SOUZA GONAÇLVES, IAC; LUCIANA LASRY BENCHIMOL, IAC. |
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Título: |
Genetic diversity of cultivated accessions and wild species of rubber tree using EST-SSR markers. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 8, p. 1087-1094, ago. 2012 |
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Páginas: |
p.1087-1094 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Diversidade genética de acessos cultivados e espécies silvestres de seringueira por meio de marcadores EST?SSR. |
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Conteúdo: |
The objective of this work was to evaluate the efficiency of EST-SSR markers in the assessment of the genetic diversity of rubber tree genotypes (Hevea brasiliensis) and to verify the transferability of these markers for wild species of Hevea. Forty-five rubber tree accessions from the Instituto Agronômico (Campinas, SP, Brazil) and six wild species were used. Information provided by modified Roger's genetic distance were used to analyze EST-SSR data. UPGMA clustering divided the samples into two major groups with high genetic differentiation, while the software Structure distributed the 51 clones into eight groups. A parallel could be established between both clustering analyses. The 30 polymorphic EST-SSRs showed from two to ten alleles and were efficient in amplifying the six wild species. Functional EST-SSR microsatellites are efficient in evaluating the genetic diversity among rubber tree clones and can be used to translate the genetic differences among cultivars and to fingerprint closely related materials. The accessions from the Instituto Agronômico show high genetic diversity. The EST-SSR markers, developed from Hevea brasiliensis, show transferability and are able to amplify other species of Hevea. |
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Palavras-Chave: |
Functional molecular markers; Genetic structure; Ingerprinting analysis; Polymorphism information content; Transferability. |
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Thesagro: |
Clone; Hevea Brasiliensis; Marcador molecular. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/76802/1/PAB-v.48-n.8-p.1087-1094.pdf
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Marc: |
LEADER 02291naa a2200301 a 4500
001 1950140
005 2017-08-21
008 2012 bl uuuu u00u1 u #d
100 1 $aPERSEGUINI, J. M. K. C
245 $aGenetic diversity of cultivated accessions and wild species of rubber tree using EST-SSR markers.
260 $c2012
300 $ap.1087-1094
500 $aTítulo em português: Diversidade genética de acessos cultivados e espécies silvestres de seringueira por meio de marcadores EST?SSR.
520 $aThe objective of this work was to evaluate the efficiency of EST-SSR markers in the assessment of the genetic diversity of rubber tree genotypes (Hevea brasiliensis) and to verify the transferability of these markers for wild species of Hevea. Forty-five rubber tree accessions from the Instituto Agronômico (Campinas, SP, Brazil) and six wild species were used. Information provided by modified Roger's genetic distance were used to analyze EST-SSR data. UPGMA clustering divided the samples into two major groups with high genetic differentiation, while the software Structure distributed the 51 clones into eight groups. A parallel could be established between both clustering analyses. The 30 polymorphic EST-SSRs showed from two to ten alleles and were efficient in amplifying the six wild species. Functional EST-SSR microsatellites are efficient in evaluating the genetic diversity among rubber tree clones and can be used to translate the genetic differences among cultivars and to fingerprint closely related materials. The accessions from the Instituto Agronômico show high genetic diversity. The EST-SSR markers, developed from Hevea brasiliensis, show transferability and are able to amplify other species of Hevea.
650 $aClone
650 $aHevea Brasiliensis
650 $aMarcador molecular
653 $aFunctional molecular markers
653 $aGenetic structure
653 $aIngerprinting analysis
653 $aPolymorphism information content
653 $aTransferability
700 1 $aROMÃO, L. R. de C.
700 1 $aBRIÑEZ, B.
700 1 $aSCALOPPI JÚNIOR, E. J.
700 1 $aGONÇALVES, P. de S.
700 1 $aBENCHIMOL, L. L.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 47, n. 8, p. 1087-1094, ago. 2012
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
04/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
CABRAL, Í dos S.; AZEVÊDO, J. A. G.; PINA, D. dos S.; PEREIRA, L. G. R.; ALMEIDA, F. M. de; SOUZA, L. L.; LIMA, R. F. de. |
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Afiliação: |
Ícaro dos Santos Cabral, UFOPA; José Augusto Gomes Azevêdo, UESC, ILHEUS; Douglas dos Santos Pina, UFBA - Bahia; LUIZ GUSTAVO RIBEIRO PEREIRA, CNPGL; Flávio Moreira de Almeida, UNIPAM; Lígia Lins Souza, UESC, ILHEUS; Ronaldo Francisco de Lima, Universidade Federal do Amazonas. |
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Título: |
Evaluation of internal markers as determinants of fecal dry matter output and digestibility in feedlot sheep. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, v. 38, n. 5, p. 3331-3339, 2017. |
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DOI: |
10.5433/1679-0359.2017v38n5p3331 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract This study aimed to evaluate the fecal recovery of the internal markers indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF), and indigestible acid detergent fiber (iADF) and to compare them as estimators of the fecal dry matter output (FDMO) and digestibility of the dry matter (DM) with values observed in total feces collection in trials with feedlot sheep. For this, 82 sampling units from two experiments were evaluated. Internal markers were quantified in the feed supplied, orts, and feces. The recovery of the markers was determined by the comparison of the amount of marker consumed and excreted. Fecal dry matter output and digestibility were determined based on the amount of marker consumed and on its concentration in the feces through individual collections directly from the rectum of the animals, with values compared with those observed in the total feces collection from orthogonal contrasts. The mean bias, the concordance correlation coefficient, and the mean squared prediction error were also evaluated. For all statistical procedures, the critical level of probability for type-I error was fixed at 0.05. The marker iDM showed complete fecal recovery (100.03%) and was efficient in estimating FDMO (482.9 g day-1) and DM digestibility (542 g kg-1) as compared with the values observed in the total feces collection (454.7 g day-1 and 561 g kg-1 for FDMO and DM digestibility, respectively). The iNDF and iADF markers did not show complete recovery in the feces and were not effective in the estimate of FDMO and DM digestibility. MenosAbstract This study aimed to evaluate the fecal recovery of the internal markers indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF), and indigestible acid detergent fiber (iADF) and to compare them as estimators of the fecal dry matter output (FDMO) and digestibility of the dry matter (DM) with values observed in total feces collection in trials with feedlot sheep. For this, 82 sampling units from two experiments were evaluated. Internal markers were quantified in the feed supplied, orts, and feces. The recovery of the markers was determined by the comparison of the amount of marker consumed and excreted. Fecal dry matter output and digestibility were determined based on the amount of marker consumed and on its concentration in the feces through individual collections directly from the rectum of the animals, with values compared with those observed in the total feces collection from orthogonal contrasts. The mean bias, the concordance correlation coefficient, and the mean squared prediction error were also evaluated. For all statistical procedures, the critical level of probability for type-I error was fixed at 0.05. The marker iDM showed complete fecal recovery (100.03%) and was efficient in estimating FDMO (482.9 g day-1) and DM digestibility (542 g kg-1) as compared with the values observed in the total feces collection (454.7 g day-1 and 561 g kg-1 for FDMO and DM digestibility, respectively). The iNDF and iADF markers did not show complete recove... Mostrar Tudo |
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Palavras-Chave: |
Fecal excretion; Indigestible components. |
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Thesaurus NAL: |
methodology. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/170341/1/Artigo-Evaluation-of-internal.pdf
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Marc: |
LEADER 02353naa a2200241 a 4500
001 2084265
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2017v38n5p3331$2DOI
100 1 $aCABRAL, Í dos S.
245 $aEvaluation of internal markers as determinants of fecal dry matter output and digestibility in feedlot sheep.$h[electronic resource]
260 $c2017
520 $aAbstract This study aimed to evaluate the fecal recovery of the internal markers indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF), and indigestible acid detergent fiber (iADF) and to compare them as estimators of the fecal dry matter output (FDMO) and digestibility of the dry matter (DM) with values observed in total feces collection in trials with feedlot sheep. For this, 82 sampling units from two experiments were evaluated. Internal markers were quantified in the feed supplied, orts, and feces. The recovery of the markers was determined by the comparison of the amount of marker consumed and excreted. Fecal dry matter output and digestibility were determined based on the amount of marker consumed and on its concentration in the feces through individual collections directly from the rectum of the animals, with values compared with those observed in the total feces collection from orthogonal contrasts. The mean bias, the concordance correlation coefficient, and the mean squared prediction error were also evaluated. For all statistical procedures, the critical level of probability for type-I error was fixed at 0.05. The marker iDM showed complete fecal recovery (100.03%) and was efficient in estimating FDMO (482.9 g day-1) and DM digestibility (542 g kg-1) as compared with the values observed in the total feces collection (454.7 g day-1 and 561 g kg-1 for FDMO and DM digestibility, respectively). The iNDF and iADF markers did not show complete recovery in the feces and were not effective in the estimate of FDMO and DM digestibility.
650 $amethodology
653 $aFecal excretion
653 $aIndigestible components
700 1 $aAZEVÊDO, J. A. G.
700 1 $aPINA, D. dos S.
700 1 $aPEREIRA, L. G. R.
700 1 $aALMEIDA, F. M. de
700 1 $aSOUZA, L. L.
700 1 $aLIMA, R. F. de
773 $tSemina: Ciências Agrárias$gv. 38, n. 5, p. 3331-3339, 2017.
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