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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
21/10/2019 |
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Data da última atualização: |
21/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
QUINTERO, F. O. C.; PINTO, L. G.; BARSALOBRES-CAVALLARI, C. F.; ARCURI, M. de L. C.; PINO, L. E.; PERES, L. E. P.; MALUF, M. P.; MAIA, I. G. |
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Afiliação: |
Fabíola O. Campo Quintero, Department of Genetics/Institute of Biosciences/Sao Paulo State University - UNESP; Layra G. Pinto, Department of Genetics/Institute of Biosciences/São Paulo State University - UNESP; Carla F. Barsalobres-Cavallari, Department of Genetics/Institute of Biosciences/São Paulo State University - UNESP; Mariana de Lara Campos Arcuri, Department of Genetics/Institute of Biosciences/São Paulo State University - UNESP; Lilian Ellen Pino, Department of Biological Sciences - LCB/Escola Superior de Agricultura 'Luiz de Queiroz' - ESALQ/University of São Paulo - USP; Lázaro Eustáquio Pereira Peres, Department of Biological Sciences - LCB/Escola Superior de Agricultura 'Luiz de Queiroz' - ESALQ/University of São Paulo - USP; MIRIAN PEREZ MALUF, CNPCa; Ivan G. Maia, Department of Genetics/Institute of Biosciences/Sao Paulo State University - UNESP. |
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Título: |
Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 37, n.9, p.1257–1268, September 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5?-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool. |
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Palavras-Chave: |
Coffee; Promoter; Seed maturation protein; Seed-specific expression; Tomato. |
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Thesagro: |
Café. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/203217/1/Identificaction-of-a-seed-maturation-protein.pdf
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Marc: |
LEADER 02110naa a2200277 a 4500
001 2113247
005 2019-10-21
008 2018 bl uuuu u00u1 u #d
100 1 $aQUINTERO, F. O. C.
245 $aIdentification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.$h[electronic resource]
260 $c2018
520 $aIn this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5?-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool.
650 $aCafé
653 $aCoffee
653 $aPromoter
653 $aSeed maturation protein
653 $aSeed-specific expression
653 $aTomato
700 1 $aPINTO, L. G.
700 1 $aBARSALOBRES-CAVALLARI, C. F.
700 1 $aARCURI, M. de L. C.
700 1 $aPINO, L. E.
700 1 $aPERES, L. E. P.
700 1 $aMALUF, M. P.
700 1 $aMAIA, I. G.
773 $tPlant Cell Reports$gv. 37, n.9, p.1257–1268, September 2018.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 6 | |
| 2. |  | ZANDONADI, D. B.; SANTOS, M. P.; BUSATO, J. G.; PERES, L. E. P; FAÇANHA, A. R. Plant physiology as affected by humified organic matter. Theoretical and Experimental Plant Physiology, Campo dos Goytacazes, v. 25, n. 1, p. 12-25, 2013. Titulo anterior: Brazilian Journal of Plant Physiology| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Hortaliças. |
|    |
| 3. | | ZANDONADI, D. B.; SANTOS, M. P.; CAIXETA, L. S.; MARINHO, E. B.; PERES, L. E. P.; FAÇANHA, A. R. Plant proton pumps as markers of biostimulant action. Scientia Agricola, Piracicaba, v. 73. n. 1, p. 24-28, Jan./Feb. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 4. | | PINO, L. E.; SOMBARDI-CRESTANA, S.; AZEVEDO, M. S.; SCOTTON, D. C.; BORGO, L.; QUECINI, V.; FIGUEIRA, A.; PERES, L. E. P. The Rg1 allele as a valuable tool for genetic transformation of the tomato 'Micro-Tom' model system. Plant Methods, London, v. 6, p. 1-11, out. 2010. Artigo número 23.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 5. | | QUINTERO, F. O. C.; PINTO, L. G.; BARSALOBRES-CAVALLARI, C. F.; ARCURI, M. de L. C.; PINO, L. E.; PERES, L. E. P.; MALUF, M. P.; MAIA, I. G. Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato. Plant Cell Reports, v. 37, n.9, p.1257–1268, September 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
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| 6. | | GONZÁLEZ-ARCOS, M.; FONSECA, M. E. N.; ZANDONADI, D. B.; PERES, L. E. P.; ARRUABARRENA, A.; FERREIRA, D. S.; KEVEI, Z.; MOHABEB, F.; THOMPSON, A. J.; BOITEUX, L. S. A loss-of-function allele of TAC1-like gene (SITAC1) located on tomato chromosome 10 is a candidate for the Erectoid leaf (Erl) mutation. Euphytica, v. 215, n. 5, p. 1-16, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 6 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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