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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
20/06/2018 |
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Data da última atualização: |
20/06/2018 |
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Autoria: |
OLIVEIRA, A. P. L.; RANGEL, J. P. P.; RAPOSO, V.; PIANCA, N. G.; CRUZ, E. P.; PEREIRA NETO, E.; FIORIO, W. A. B.; MONTEIRO, B. S. |
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Afiliação: |
Alvaro P. L. Oliveira, Laboratório de Terapia Celular e Células Tronco/Departamento de Medicina Veterinária/Universidade Vila Velha - UVV; Julia P. P. Rangel, Laboratório de Terapia Celular e Células Tronco/Departamento de Medicina Veterinária/Universidade Vila Velha - UVV; Viviane Raposo, Laboratório de Terapia Celular e Células/Departamento de Medicina Veterinária - UVV; Noely G. Pianca, Iniciação Científica/Laboratório de Terapia Celular e Células/Departamento de Medicina Veterinária - UVV; Emanuelle P. Cruz, Iniciação Científica/Laboratório de Terapia Celular e Células/Departamento de Medicina Veterinária - UVV; Evandro Pereira Neto, Laboratório de Terapia Celular e Células Tronco/Departamento de Medicina Veterinária/Universidade Vila Velha - UVV; Wagner A. B. Fiorio, Laboratório de Terapia Celular e Células Tronco/Departamento de Medicina Veterinária/Universidade Vila Velha - UVV; Betânia S. Monteiro, Laboratório de Terapia Celular e Células/Departamento de Medicina Veterinária - UVV. |
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Título: |
Allogenic mesenchymal stem cell intravenous infusion in reparation of mild intestinal ischemia/reperfusion injury in New Zealand rabbits. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 710-721, abril 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Infusão intravenosa de células tronco mesenquimais alógenas na reparação da lesão branda de isquemia/reperfusão intestinal em coelhos Nova Zelândia. |
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Conteúdo: |
The present study aimed to evaluate the efficacy of mesenchymal stem cell (MSC) infusion, derived from adipose tissue, on reduction of local and remote tissue damage caused by the event of experimental intestinal I/R in New Zealand breed rabbits. For obtaining, characterization, and cultivation of MSC derived from adipose tissue (MSC-Adp), 3 juvenile animals (four months old) were used. The cells were considered to be viable for therapy after the fourth passage (in vitro phase). For the in vivo stage, 24 young adult animals (six months old) were used, weighing approximately 3.5 kg, in which were randomly divided into two groups, called: IR treated with MSC (I2H/R5H MSC 3D; I2H/R5H MSC 7D); IR treated with PBS (I2H/R5H PBS 3D; I2H/R5H PBS 7D). The animals were anesthetized and submitted to pre-retro-umbilical midline celiotomy. The extramural peri-intestinal marginal artery was located and clamped (predetermined and standardized region) with the aid of a vascular clip, promoting a 2 hour blood flow interruption. After this period, blood flow was reestablished, inhalatory anesthesia was suspended, and the animals awaken. After 5 hours of reperfusion, the treatments were performed by intravenous infusion according to the experimental groups. The animals were evaluated 72 hours and seven days after the treatment as for the macroscopic appearance (color and peristaltism) of the jejunal segment, and by histological evaluation of the ischemic segment for the presence or absence of destruction of the intestinal mucosa, edema, bleeding, dilation of lymph vessels, and presence of polymorphonuclear inflammatory cells, both in the mucosa and submucosa. The observed results revealed that the groups treated with MSC-Adp obtained smaller mucosal and submucosal lesions when compared to the groups treated with PBS. Also, MSC-Adp treated groups obtained controlled inflammatory response and higher mitotic rate, outcomes related to the therapeutic potential of MSC. Infusion of stem cells attenuated the lesions caused by intestinal I/R in both MSC groups when compared to the group treated with PBS. MenosThe present study aimed to evaluate the efficacy of mesenchymal stem cell (MSC) infusion, derived from adipose tissue, on reduction of local and remote tissue damage caused by the event of experimental intestinal I/R in New Zealand breed rabbits. For obtaining, characterization, and cultivation of MSC derived from adipose tissue (MSC-Adp), 3 juvenile animals (four months old) were used. The cells were considered to be viable for therapy after the fourth passage (in vitro phase). For the in vivo stage, 24 young adult animals (six months old) were used, weighing approximately 3.5 kg, in which were randomly divided into two groups, called: IR treated with MSC (I2H/R5H MSC 3D; I2H/R5H MSC 7D); IR treated with PBS (I2H/R5H PBS 3D; I2H/R5H PBS 7D). The animals were anesthetized and submitted to pre-retro-umbilical midline celiotomy. The extramural peri-intestinal marginal artery was located and clamped (predetermined and standardized region) with the aid of a vascular clip, promoting a 2 hour blood flow interruption. After this period, blood flow was reestablished, inhalatory anesthesia was suspended, and the animals awaken. After 5 hours of reperfusion, the treatments were performed by intravenous infusion according to the experimental groups. The animals were evaluated 72 hours and seven days after the treatment as for the macroscopic appearance (color and peristaltism) of the jejunal segment, and by histological evaluation of the ischemic segment for the presence or absence of ... Mostrar Tudo |
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Palavras-Chave: |
Artéria marginal peri-intestinal extramural; Cell therapy; Células tronco mesenquimais; Clínica; Extramural peri-intestinal; Intestinal ischemia; Intravenous infusion; Isquemia intestinal; Lesão remota; New Zealand rabbit; Remote injury; Terapia celular. |
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Thesagro: |
Coelho Nova Zelândia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178809/1/Allogenic-mesenchymal-stem-cell-intravenous-infusion.pdf
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Marc: |
LEADER 03477naa a2200373 a 4500
001 2092621
005 2018-06-20
008 2018 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, A. P. L.
245 $aAllogenic mesenchymal stem cell intravenous infusion in reparation of mild intestinal ischemia/reperfusion injury in New Zealand rabbits.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Infusão intravenosa de células tronco mesenquimais alógenas na reparação da lesão branda de isquemia/reperfusão intestinal em coelhos Nova Zelândia.
520 $aThe present study aimed to evaluate the efficacy of mesenchymal stem cell (MSC) infusion, derived from adipose tissue, on reduction of local and remote tissue damage caused by the event of experimental intestinal I/R in New Zealand breed rabbits. For obtaining, characterization, and cultivation of MSC derived from adipose tissue (MSC-Adp), 3 juvenile animals (four months old) were used. The cells were considered to be viable for therapy after the fourth passage (in vitro phase). For the in vivo stage, 24 young adult animals (six months old) were used, weighing approximately 3.5 kg, in which were randomly divided into two groups, called: IR treated with MSC (I2H/R5H MSC 3D; I2H/R5H MSC 7D); IR treated with PBS (I2H/R5H PBS 3D; I2H/R5H PBS 7D). The animals were anesthetized and submitted to pre-retro-umbilical midline celiotomy. The extramural peri-intestinal marginal artery was located and clamped (predetermined and standardized region) with the aid of a vascular clip, promoting a 2 hour blood flow interruption. After this period, blood flow was reestablished, inhalatory anesthesia was suspended, and the animals awaken. After 5 hours of reperfusion, the treatments were performed by intravenous infusion according to the experimental groups. The animals were evaluated 72 hours and seven days after the treatment as for the macroscopic appearance (color and peristaltism) of the jejunal segment, and by histological evaluation of the ischemic segment for the presence or absence of destruction of the intestinal mucosa, edema, bleeding, dilation of lymph vessels, and presence of polymorphonuclear inflammatory cells, both in the mucosa and submucosa. The observed results revealed that the groups treated with MSC-Adp obtained smaller mucosal and submucosal lesions when compared to the groups treated with PBS. Also, MSC-Adp treated groups obtained controlled inflammatory response and higher mitotic rate, outcomes related to the therapeutic potential of MSC. Infusion of stem cells attenuated the lesions caused by intestinal I/R in both MSC groups when compared to the group treated with PBS.
650 $aCoelho Nova Zelândia
653 $aArtéria marginal peri-intestinal extramural
653 $aCell therapy
653 $aCélulas tronco mesenquimais
653 $aClínica
653 $aExtramural peri-intestinal
653 $aIntestinal ischemia
653 $aIntravenous infusion
653 $aIsquemia intestinal
653 $aLesão remota
653 $aNew Zealand rabbit
653 $aRemote injury
653 $aTerapia celular
700 1 $aRANGEL, J. P. P.
700 1 $aRAPOSO, V.
700 1 $aPIANCA, N. G.
700 1 $aCRUZ, E. P.
700 1 $aPEREIRA NETO, E.
700 1 $aFIORIO, W. A. B.
700 1 $aMONTEIRO, B. S.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 4, p. 710-721, abril 2018
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Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
16/09/1996 |
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Data da última atualização: |
13/03/2008 |
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Autoria: |
MENOSSO, O. G.; COSTA, J. A.; BOHNEN, H.; ANGHINONI, I. |
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Afiliação: |
Embrapa-Soja. Londrina, PR. |
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Título: |
Mecanismo de tolerancia ao aluminio em soja: III. conteudo de acidos organicos no sistema de raizes. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
In: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 17., 1995, Goiania. Ata e resumos. Goiania: EMGOPA, 1995. |
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Páginas: |
p.183-184. |
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Série: |
(EMGOPA. Documentos, 28). |
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Idioma: |
Português |
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Palavras-Chave: |
Aluminium; Brasil; Root system; Soybean; Tolerance; Tolerancia. |
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Thesagro: |
Alumínio; Sistema Radicular; Soja. |
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Thesaurus NAL: |
Brazil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00839naa a2200289 a 4500
001 1456211
005 2008-03-13
008 1995 bl uuuu u00u1 u #d
100 1 $aMENOSSO, O. G.
245 $aMecanismo de tolerancia ao aluminio em soja$bIII. conteudo de acidos organicos no sistema de raizes.
260 $c1995
300 $ap.183-184.
490 $a(EMGOPA. Documentos, 28).
650 $aBrazil
650 $aAlumínio
650 $aSistema Radicular
650 $aSoja
653 $aAluminium
653 $aBrasil
653 $aRoot system
653 $aSoybean
653 $aTolerance
653 $aTolerancia
700 1 $aCOSTA, J. A.
700 1 $aBOHNEN, H.
700 1 $aANGHINONI, I.
773 $tIn: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 17., 1995, Goiania. Ata e resumos. Goiania: EMGOPA, 1995.
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