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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
31/03/2016 |
Data da última atualização: |
26/01/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FREITAS NETO, M.; PEREIRA, T. N. S.; GERONIMO, I. G. C.; AZEVEDO, A. O. N.; RAMOS, S. R. R.; PEREIRA, M. G. |
Título: |
Coconut genome size determined by flow cytometry: tall versus dwarf types. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 15, n. 1, february, 2016. |
DOI: |
http://dx.doi.org/10.4238/gmr.15017470 |
Idioma: |
Português |
Conteúdo: |
Coconuts (Cocos nucifera L.) are tropical palm trees that are classified into Tall and Dwarf types based on height, and both types are diploid (2n = 2x = 32 chromosomes). The reproduction mode is autogamous for Dwarf types and allogamous for Tall types. One hypothesis for the origin of the Dwarf coconut suggests that it is a Tall variant that resulted from either mutation or inbreeding, and differences in genome size between the two types would support this hypothesis. In this study, we estimated the genome sizes of 14 coconut accessions (eight Tall and six Dwarf types) using flow cytometry. Nuclei were extracted from leaf discs and stained with propidium iodide, and Pisum sativum (2C = 9.07 pg DNA) was used as an internal standard. Histograms with good resolution and low coefficients of variation (2.5 to 3.2%) were obtained. The 2C DNA content ranged from 5.72 to 5.48 pg for Tall accessions and from 5.58 to 5.52 pg for Dwarf accessions. The mean genome sizes for Tall and Dwarf specimens were 5.59 and 5.55 pg, respectively. Among all accessions, Rennel Island Tall had the highest mean DNA content (5.72 pg), whereas West African Tall had the lowest (5.48 pg). The mean coconut genome size (2C = 5.57 pg, 2M. Freitas Neto et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (1): gmr.15017470 corresponding to 2723.73 Mbp/haploid set) was classified as small. Only small differences in genome size existed among the coconut accessions, suggesting that the Dwarf type did not evolve from the Tall type. MenosCoconuts (Cocos nucifera L.) are tropical palm trees that are classified into Tall and Dwarf types based on height, and both types are diploid (2n = 2x = 32 chromosomes). The reproduction mode is autogamous for Dwarf types and allogamous for Tall types. One hypothesis for the origin of the Dwarf coconut suggests that it is a Tall variant that resulted from either mutation or inbreeding, and differences in genome size between the two types would support this hypothesis. In this study, we estimated the genome sizes of 14 coconut accessions (eight Tall and six Dwarf types) using flow cytometry. Nuclei were extracted from leaf discs and stained with propidium iodide, and Pisum sativum (2C = 9.07 pg DNA) was used as an internal standard. Histograms with good resolution and low coefficients of variation (2.5 to 3.2%) were obtained. The 2C DNA content ranged from 5.72 to 5.48 pg for Tall accessions and from 5.58 to 5.52 pg for Dwarf accessions. The mean genome sizes for Tall and Dwarf specimens were 5.59 and 5.55 pg, respectively. Among all accessions, Rennel Island Tall had the highest mean DNA content (5.72 pg), whereas West African Tall had the lowest (5.48 pg). The mean coconut genome size (2C = 5.57 pg, 2M. Freitas Neto et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (1): gmr.15017470 corresponding to 2723.73 Mbp/haploid set) was classified as small. Only small differences in genome size existed among the coconut accessions, suggesting that the Dwarf ty... Mostrar Tudo |
Palavras-Chave: |
C-value; Coconut accessions; Cocos nucifera L; DNA content; Palm tree. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141883/1/gmr7470.pdf
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Marc: |
LEADER 02294naa a2200253 a 4500 001 2042274 005 2018-01-26 008 2016 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.4238/gmr.15017470$2DOI 100 1 $aFREITAS NETO, M. 245 $aCoconut genome size determined by flow cytometry$btall versus dwarf types.$h[electronic resource] 260 $c2016 520 $aCoconuts (Cocos nucifera L.) are tropical palm trees that are classified into Tall and Dwarf types based on height, and both types are diploid (2n = 2x = 32 chromosomes). The reproduction mode is autogamous for Dwarf types and allogamous for Tall types. One hypothesis for the origin of the Dwarf coconut suggests that it is a Tall variant that resulted from either mutation or inbreeding, and differences in genome size between the two types would support this hypothesis. In this study, we estimated the genome sizes of 14 coconut accessions (eight Tall and six Dwarf types) using flow cytometry. Nuclei were extracted from leaf discs and stained with propidium iodide, and Pisum sativum (2C = 9.07 pg DNA) was used as an internal standard. Histograms with good resolution and low coefficients of variation (2.5 to 3.2%) were obtained. The 2C DNA content ranged from 5.72 to 5.48 pg for Tall accessions and from 5.58 to 5.52 pg for Dwarf accessions. The mean genome sizes for Tall and Dwarf specimens were 5.59 and 5.55 pg, respectively. Among all accessions, Rennel Island Tall had the highest mean DNA content (5.72 pg), whereas West African Tall had the lowest (5.48 pg). The mean coconut genome size (2C = 5.57 pg, 2M. Freitas Neto et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (1): gmr.15017470 corresponding to 2723.73 Mbp/haploid set) was classified as small. Only small differences in genome size existed among the coconut accessions, suggesting that the Dwarf type did not evolve from the Tall type. 653 $aC-value 653 $aCoconut accessions 653 $aCocos nucifera L 653 $aDNA content 653 $aPalm tree 700 1 $aPEREIRA, T. N. S. 700 1 $aGERONIMO, I. G. C. 700 1 $aAZEVEDO, A. O. N. 700 1 $aRAMOS, S. R. R. 700 1 $aPEREIRA, M. G. 773 $tGenetics and Molecular Research$gv. 15, n. 1, february, 2016.
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Embrapa Tabuleiros Costeiros (CPATC) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
Data corrente: |
25/11/2002 |
Data da última atualização: |
25/11/2002 |
Autoria: |
BRAZ FILHO, R.; GOTTLIEB, O. R.; MORAES, A. A.; PEDREIRA, G.; PINHO, S. L. V.; MAGALHAES, M. T.; RIBEIRO, M. N. S. |
Título: |
The Chemistry of Brazilian Myristicaceae. IX. Isoflavonoids from Amazonian species. |
Ano de publicação: |
1977 |
Fonte/Imprenta: |
Lloydia, Columbus, v. 40, p. 236-240, 1977. |
Idioma: |
Inglês |
Palavras-Chave: |
Especies nativas; Isoflavonoides; Myristicaceas; Produto naturais. |
Thesagro: |
Química. |
Thesaurus NAL: |
Amazonia. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00701naa a2200253 a 4500 001 1414273 005 2002-11-25 008 1977 bl --- 0-- u #d 100 1 $aBRAZ FILHO, R. 245 $aThe Chemistry of Brazilian Myristicaceae. IX. Isoflavonoids from Amazonian species. 260 $c1977 650 $aAmazonia 650 $aQuímica 653 $aEspecies nativas 653 $aIsoflavonoides 653 $aMyristicaceas 653 $aProduto naturais 700 1 $aGOTTLIEB, O. R. 700 1 $aMORAES, A. A. 700 1 $aPEDREIRA, G. 700 1 $aPINHO, S. L. V. 700 1 $aMAGALHAES, M. T. 700 1 $aRIBEIRO, M. N. S. 773 $tLloydia, Columbus$gv. 40, p. 236-240, 1977.
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