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Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
05/10/2011 |
Data da última atualização: |
10/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CAMPOS, M. K. F. de; CARVALHO, K. de; SOUZA, F. S. de; MARUR, C. J.; PEREIRA, L. F. P.; BESPALHOK FILHO, J. C.; VIEIRA, L. G. E. |
Afiliação: |
INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ; LUIZ FILIPE PROTASIO PEREIRA, SAPC; UNIVERSIDADE FEDERAL DO PARANÁ; UNIVERSIDADE FEDERAL DO PARANÁ. |
Título: |
Drought tolerance and antioxidant enzymatic activity in transgenic 'Swingle' citrumelo plants over-accumulating proline. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Environmental and Experimental Botany, v.72, n. 2, p. 242-250, 2011. |
Idioma: |
Inglês |
Conteúdo: |
In this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the following conditions: irrigated (w = -1.3 MPa), moderate stress (w = -2.3 to - 2.5 MPa), severe stress (w = -3.8 to - 3.9 MPa) and recovery (24 h after re-irrigation: w = -1.3 to - 1.9 MPa). Osmotic adjustment was observed in transgenic plants until 11 days after withholding water, while pressure potential in non-transformed controls was close to zero after nine days of water deprivation. This superior maintenance of turgor pressure in leaves of transgenic plants led to higher stomatal conductance, photosynthetic and transpiration rates when compared to non-transgenic plants. Drought caused a significant decrease in APX and SOD activities in control plants, followed by an increase after re-watering. On the other hand, CAT was more active in control than in transgenic plants under irrigated condition and both stress levels. Our results suggest that transgenic plants were able to cope with water deficit better than non-transformed controls since the high endogenous proline level acted not only by mediating osmotic adjustment, but also by contributing to gas exchange parameters and ameliorating deleterious effects of drought-induced oxidative stress. MenosIn this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the following conditions: irrigated (w = -1.3 MPa), moderate stress (w = -2.3 to - 2.5 MPa), severe stress (w = -3.8 to - 3.9 MPa) and recovery (24 h after re-irrigation: w = -1.3 to - 1.9 MPa). Osmotic adjustment was observed in transgenic plants until 11 days after withholding water, while pressure potential in non-transformed controls was close to zero after nine days of water deprivation. This superior maintenance of turgor pressure in leaves of transgenic plants led to higher stomatal conductance, photosynthetic and transpiration rates when compared to non-transgenic plants. Drought caused a significant decrease in APX and SOD activities in control plants, followed by an increase after re-watering. On the other hand, CAT was m... Mostrar Tudo |
Palavras-Chave: |
Citrus rootstock; Osmotic adjustment. |
Thesaurus Nal: |
Drought tolerance; Oxidative stress; Proline. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42905/1/Drought-tolerance-and-antioxidant.pdf
|
Marc: |
LEADER 02726naa a2200253 a 4500 001 1902435 005 2024-01-10 008 2011 bl uuuu u00u1 u #d 100 1 $aCAMPOS, M. K. F. de 245 $aDrought tolerance and antioxidant enzymatic activity in transgenic 'Swingle' citrumelo plants over-accumulating proline.$h[electronic resource] 260 $c2011 520 $aIn this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the following conditions: irrigated (w = -1.3 MPa), moderate stress (w = -2.3 to - 2.5 MPa), severe stress (w = -3.8 to - 3.9 MPa) and recovery (24 h after re-irrigation: w = -1.3 to - 1.9 MPa). Osmotic adjustment was observed in transgenic plants until 11 days after withholding water, while pressure potential in non-transformed controls was close to zero after nine days of water deprivation. This superior maintenance of turgor pressure in leaves of transgenic plants led to higher stomatal conductance, photosynthetic and transpiration rates when compared to non-transgenic plants. Drought caused a significant decrease in APX and SOD activities in control plants, followed by an increase after re-watering. On the other hand, CAT was more active in control than in transgenic plants under irrigated condition and both stress levels. Our results suggest that transgenic plants were able to cope with water deficit better than non-transformed controls since the high endogenous proline level acted not only by mediating osmotic adjustment, but also by contributing to gas exchange parameters and ameliorating deleterious effects of drought-induced oxidative stress. 650 $aDrought tolerance 650 $aOxidative stress 650 $aProline 653 $aCitrus rootstock 653 $aOsmotic adjustment 700 1 $aCARVALHO, K. de 700 1 $aSOUZA, F. S. de 700 1 $aMARUR, C. J. 700 1 $aPEREIRA, L. F. P. 700 1 $aBESPALHOK FILHO, J. C. 700 1 $aVIEIRA, L. G. E. 773 $tEnvironmental and Experimental Botany$gv.72, n. 2, p. 242-250, 2011.
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Embrapa Café (CNPCa) |
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81. | | SCHOLZ, M. B. dos S.; KITZBERGER, C. S. G.; PAGIATTO, N. F.; PEREIRA, L. F. P.; DAVRIEUX, F.; POT, D.; CHARMETANT, P.; LEROY, T. Chemical composition in wild ethiopian Arabica coffee accessions. Euphytica, v. 209, n. 2 p. 429-438, 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Café. |
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82. | | DIOLA, V.; LOUREIRO, M. E.; BRITO, G. G. de; CAIXETA, E. T.; ZAMBOLIN, E. M.; SAKIYAMA, N. S.; PEREIRA, L. F. P. Construção do mapa genético e físico para um gene de resistência a Hemileia vastatrix em café. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para produção de alimentos e bioenergia. Fortaleza: SBFV: UFC: Embrapa Agroindústria Tropical, 2009.Tipo: Resumo em Anais de Congresso |
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83. | | CAÇÃO, S. M. B.; DINIZ, L. E. C.; SILVA. N. V.; VENIKY, F.; ANDRADE, A. C.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Construção de uma biblioteca genômica de cromossomo artificial de bactéria de Coffea arabica. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007. Biotecnologia aplicada à cadeia agroindustrial do café.Tipo: Artigo em Anais de Congresso / Nota Técnica |
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84. | | CAÇÃO, S. M. B.; DINIZ, L. E. C.; SILVA, N. V.; VENIKY, F.; ANDRADE, A. C.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Construção de uma biblioteca genômica de cromossomo artificial de bactéria de Coffea arabica. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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85. | | PEREIRA, L. F. P.; CAÇÃO, S. M. B.; SILVA, N. V.; DINIZ, L. E. C.; ALVES, G. S. C.; VINECKY, F.; ANDRADE, A. C.; VIEIRA, L. G. E. Construction of a Coffea arabica bacterial artificial chromosome library. In: ENCUENTRO LATINO AMERICANO Y DEL CARIBE DE BIOTECNOLOGIA AGROPECUARIA: REDBIO 2007, 6., 2007, Viña del Mar, Chile. Abstracts and posters? Roma: FAO, 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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86. | | DOMINGUES, D. S.; MEDA, A. R.; SANTOS, T. B.; VESPERO, E. B.; SITTA, R. B.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Coffea nutriome: minding the gap among genomics, biotechnology and fertilization practices. In.: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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87. | | IVAMOTO, S. T.; POT, D.; CHARMETANT, P.; MARRACCINI, P.; FERREIRA, L. P.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P. Diterpenes in Coffea arabica: biochemical aspects and transcriptional analysis of candidate cyps involved in cafestol and kahweol biosynthesis. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 216Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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88. | | KITZBERGER, C. S. G.; SCHOLZ, M. B. dos S.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; SERA, T.; SILVA, J. B. G. D.; BENASSI, M. de T. Diterpenes in green and roasted coffee of Coffea arabica cultivars growing in the same edapho-climatic conditions. Journal of Food Composition and Analysis, v. 30, n. 1, P. 52-57, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Café. |
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89. | | SILVA, C. R. M. da; LOPES, F. R.; CARARETO, C. M. A.; VANZELA, A. L. L.; DOMINGUES, D. S.; PEREIRA, L. F. P. Distribuição de elementos transponíveis em cromossomos de coffea arabica In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.Tipo: Artigo em Anais de Congresso |
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90. | | GARRETT, R.; SCHMIDT, E. M.; PEREIRA, L. F. P.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; EBERLIN, M. N.; REZENDE, C. M. Discrimination of arabica coffee cultivars by electrospray ionization fourier transform ion cyclotron resonance mass spectrometry and chemometrics. LWT - Food Science and Technology, v. 50, n. 2, p. 496-502, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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91. | | CAMPOS, M. K. F. de; CARVALHO, K. de; SOUZA, F. S. de; MARUR, C. J.; PEREIRA, L. F. P.; BESPALHOK FILHO, J. C.; VIEIRA, L. G. E. Drought tolerance and antioxidant enzymatic activity in transgenic 'Swingle' citrumelo plants over-accumulating proline. Environmental and Experimental Botany, v.72, n. 2, p. 242-250, 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Café. |
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92. | | IVAMOTO, S. T.; CALEDÓN, J. M.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; BOHLMANN, J.; DOMINGUES, D. S.; PEREIRA, L. F. P. Transcriptome analysis and characterization of genes involved in diterpene biosynthesis in coffea arabica. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 105Tipo: Resumo em Anais de Congresso |
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93. | | YUYAMA, P. M.; REIS JUNIOR, O.; IVAMOTO, S. T.; DOMINGUES, D. S.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; CHARMETANT, P.; LEROY, T.; PEREIRA, L. F. P. Transcriptome analysis in Coffea eugenioides, an Arabica coffee ancestor, reveals differentially expressed genes inleaves and fruits. Molecular Genetics and Genomics, v. 291, n. 1, p. 323-336. 2016Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
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94. | | SCHOLZ, M. B. S.; PAGIATTO, N. F.; KITZBERGER, C. S. G.; PEREIRA, L. F. P.; DAVRIEUX, F.; CHARMETANTE, P.; LEROYE, T. Validation of near-infrared spectroscopy for the quantification of cafestol and kahweol in green coffee. Food Research International, v. 61, p. 176-182, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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95. | | PEREIRA, L. F. P.; CAÇÃO, S. M. B.; SILVA, N. V.; ALVES, G. S. C.; VINECKY, F.; CAIXETA, E. T.; ANDRADE, A. C.; VIEIRA, L. G. E. Using a coffea arabica bacterial artificial chromosome library for gene cloning and integrative mapping approaches. In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego, 2008, p. 276.Tipo: Resumo em Anais de Congresso |
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96. | | CAÇÃO, S. M. B.; SILVA, N. V.; ALVES, G. S. C.; VINECKY, F.; DINIZ, L. E. C.; ANDRADE, A. C.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Using coffea arabica bacterial artificial chromosome library for gene cloning and integrative mapping aproaches. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas, Brazil. Programme abstracts. Montpellier, France: Association for Science and Information on Coffee, 2008. PB633.Tipo: Resumo em Anais de Congresso |
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97. | | CAÇÃO, S. M. B.; SILVA, N. V.; ALVES, G. S. C.; VINIECK, F.; DINIZ, L. E. C.; ANDRADE, A. C.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Using a Coffea arabica bacterial artificial chromosome library for gene cloning and integrative mapping aproaches. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas, Brazil.Tipo: Resumo em Anais de Congresso |
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99. | | GEROMEL, C.; FERREIRA, L. P.; CAVALARI, A. A.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; LEROY, T.; MAZZAFERA, P.; MARRACCINI, P. Sugar metabolism during coffee fruit development. In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 20., 2004, Bangalore, India. Anais... Montpellier, France: Association for Science and Information on Coffee, 2005. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
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100. | | PAGIATTO, N. F.; IVAMOTO, S. T.; SERA, T.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; CHARMETANT, P.; LEROY, T.; DOMINGUES, D.; PEREIRA, L. F. P. Evaluation of kahweol and cafestol levels in Ethiopian Coffea arabica L. accessions grown in Brazil. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 217Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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Registros recuperados : 171 | |
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