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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/03/2017 |
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Data da última atualização: |
29/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BALZON, T. A.; LUIS, Z. G.; PEREIRA, J. E. S. |
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Afiliação: |
Talita Aparecida Balzon; Zanderluce Gomes Luis, UnB; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
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Título: |
New approaches to improve the efficiency of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.) from mature zygotic embryos. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In Vitro Cellular e Developmental Biology, v. 49, p. 41-50, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
We developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from mature zygotic embryos of oil palm. Embryogenic calli were induced from mature zygotic embryos of oil palm on modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid or picloram, alone or in combination with activated charcoal. The greatest frequency of embryogenic callus induction (97.5%) was obtained by culturing mature zygotic embryos on callus induction medium with 450 μM picloram and 2.5 gL−1 activated charcoal. Embryogenic calli proliferated on a medium with a reduced concentration of picloram. Embryogenic calli were then subcultured on a medium supplemented with 12.3 μM 2-isopentenyladenine and 0.54 μM naphthaleneacetic acid, with subcultures at 4-wk intervals. Somatic embryos were regenerated on a medium with Murashige and Skoog macro- and micronutrients at halfstrength concentrations supplemented with 20 gL−1 sucrose, 2.5 gL−1 activated charcoal, and 2.5 gL−1 Phytagel. Detailed histological analysis revealed that somatic embryogenesis followed an indirect pathway. Primary calli were observed after 4?6 wk of culture and progressed to embryogenic calli at 12 wk. Embryogenic cells exhibited dense protoplasm, a high nucleoplasmic ratio, and small starch grains. Proembryos, which seemed to have a multicellular origin, formed after 16?20 wk of culture and successive cell divisions. Differentiated somatic embryos had a haustorium, a plumule, and the first and second foliar sheaths. In differentiated embryos, the radicular protrusion was not apparent because it generally does not appear until after the first true leaves emerge. MenosWe developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from mature zygotic embryos of oil palm. Embryogenic calli were induced from mature zygotic embryos of oil palm on modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid or picloram, alone or in combination with activated charcoal. The greatest frequency of embryogenic callus induction (97.5%) was obtained by culturing mature zygotic embryos on callus induction medium with 450 μM picloram and 2.5 gL−1 activated charcoal. Embryogenic calli proliferated on a medium with a reduced concentration of picloram. Embryogenic calli were then subcultured on a medium supplemented with 12.3 μM 2-isopentenyladenine and 0.54 μM naphthaleneacetic acid, with subcultures at 4-wk intervals. Somatic embryos were regenerated on a medium with Murashige and Skoog macro- and micronutrients at halfstrength concentrations supplemented with 20 gL−1 sucrose, 2.5 gL−1 activated charcoal, and 2.5 gL−1 Phytagel. Detailed histological analysis revealed that somatic embryogenesis followed an indirect pathway. Primary calli were observed after 4?6 wk of culture and progressed to embryogenic calli at 12 wk. Embryogenic cells exhibited dense protoplasm, a high nucleoplasmic ratio, and small starch grains. Proembryos, which seemed to have a multicellular origin, formed after 16?20 wk of culture and successive cell divisions. Differentiated somatic embr... Mostrar Tudo |
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Palavras-Chave: |
Zygotic embryo. |
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Thesaurus Nal: |
morphogenesis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180927/1/Balzon2013-Article-NewApproachesToImproveTheEffic.pdf
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Marc: |
LEADER 02287naa a2200169 a 4500
001 2067490
005 2023-03-29
008 2013 bl uuuu u00u1 u #d
100 1 $aBALZON, T. A.
245 $aNew approaches to improve the efficiency of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.) from mature zygotic embryos.$h[electronic resource]
260 $c2013
520 $aWe developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from mature zygotic embryos of oil palm. Embryogenic calli were induced from mature zygotic embryos of oil palm on modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid or picloram, alone or in combination with activated charcoal. The greatest frequency of embryogenic callus induction (97.5%) was obtained by culturing mature zygotic embryos on callus induction medium with 450 μM picloram and 2.5 gL−1 activated charcoal. Embryogenic calli proliferated on a medium with a reduced concentration of picloram. Embryogenic calli were then subcultured on a medium supplemented with 12.3 μM 2-isopentenyladenine and 0.54 μM naphthaleneacetic acid, with subcultures at 4-wk intervals. Somatic embryos were regenerated on a medium with Murashige and Skoog macro- and micronutrients at halfstrength concentrations supplemented with 20 gL−1 sucrose, 2.5 gL−1 activated charcoal, and 2.5 gL−1 Phytagel. Detailed histological analysis revealed that somatic embryogenesis followed an indirect pathway. Primary calli were observed after 4?6 wk of culture and progressed to embryogenic calli at 12 wk. Embryogenic cells exhibited dense protoplasm, a high nucleoplasmic ratio, and small starch grains. Proembryos, which seemed to have a multicellular origin, formed after 16?20 wk of culture and successive cell divisions. Differentiated somatic embryos had a haustorium, a plumule, and the first and second foliar sheaths. In differentiated embryos, the radicular protrusion was not apparent because it generally does not appear until after the first true leaves emerge.
650 $amorphogenesis
653 $aZygotic embryo
700 1 $aLUIS, Z. G.
700 1 $aPEREIRA, J. E. S.
773 $tIn Vitro Cellular e Developmental Biology$gv. 49, p. 41-50, 2013.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
05/09/2011 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
LOPES, M. N.; CÂNDIDO, M. J. D.; POMPEU, R. C. F. F.; SILVA, R. G. da; LOPES, J. W. B.; BEZERRA, F. M. L. |
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Afiliação: |
Marcos Neves Lopes, Universidade Federal do Ceará - UFC, Fortaleza, CE; Magno José Duarte Cândido, UFC, Fortaleza, CE.; ROBERTO CLAUDIO FERNANDES F POMPEU, CNPC; Rodrigo Gregório da Silva, Instituto Federal de Educação, Ciência e Tecnologia – IFCE, Campus Avançado do Tauá, CE.; José Wellington Batista Lopes, UFC, Fortaleza, CE.; Francisco Marcus Lima Bezerra, UFC, Fortaleza, CE. |
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Título: |
Biomass components in Massai grass during establishment and regrowth under five nitrogen fertilization levels. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 40, n. 8, p. 1629-1637, 2011. |
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DOI: |
http://dx.doi.org/10.1590/S1516-35982011000800002 |
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Idioma: |
Inglês |
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Conteúdo: |
To evaluate biomass components of Massai grass under five doses of N (0, 150, 300, 450 and 600 mg N/dm3 soil) during three cycles of growth (establishment, regrowth 1 and regrowth 2) this study was carried out. The experiment was carried out in a greenhouse, in a completely randomized design with measures repeted over time and five replications. In the three cycles, nitrogen doses had increasing linear effect on green herbage dry matter (GHDM), green leaf dry matter (GLDM), on the tiller population density (TPD) and on forage density. Canopy height and foliage height were the highest ones in establishment of regrowth 2, respectively, and they decreased at the highest doses of nitrogen. Irrigation depth was the greatest at the highest doses and in regrowth 1. Water use efficiency responded positively to the doses, being the regrowth 2 the highest values were obtained at the highest doses of nitrogen. Nitrogen use efficiency by Massai grass is influenced by fertilizantion as well as by the cycles. Nitrogen fertilization has positive effects on the components of Massai grass biomass. |
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Palavras-Chave: |
Fertilização; Fertilization; Forage dry matter; Variedade Massai. |
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Thesagro: |
Biomassa; Gramínea forrageira; Matéria seca; Panicum maximum; Pastagem. |
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Thesaurus NAL: |
Biomass. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/41163/1/API-Biomass.pdf
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Marc: |
LEADER 02059naa a2200313 a 4500
001 1899725
005 2019-09-23
008 2011 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/S1516-35982011000800002$2DOI
100 1 $aLOPES, M. N.
245 $aBiomass components in Massai grass during establishment and regrowth under five nitrogen fertilization levels.$h[electronic resource]
260 $c2011
520 $aTo evaluate biomass components of Massai grass under five doses of N (0, 150, 300, 450 and 600 mg N/dm3 soil) during three cycles of growth (establishment, regrowth 1 and regrowth 2) this study was carried out. The experiment was carried out in a greenhouse, in a completely randomized design with measures repeted over time and five replications. In the three cycles, nitrogen doses had increasing linear effect on green herbage dry matter (GHDM), green leaf dry matter (GLDM), on the tiller population density (TPD) and on forage density. Canopy height and foliage height were the highest ones in establishment of regrowth 2, respectively, and they decreased at the highest doses of nitrogen. Irrigation depth was the greatest at the highest doses and in regrowth 1. Water use efficiency responded positively to the doses, being the regrowth 2 the highest values were obtained at the highest doses of nitrogen. Nitrogen use efficiency by Massai grass is influenced by fertilizantion as well as by the cycles. Nitrogen fertilization has positive effects on the components of Massai grass biomass.
650 $aBiomass
650 $aBiomassa
650 $aGramínea forrageira
650 $aMatéria seca
650 $aPanicum maximum
650 $aPastagem
653 $aFertilização
653 $aFertilization
653 $aForage dry matter
653 $aVariedade Massai
700 1 $aCÂNDIDO, M. J. D.
700 1 $aPOMPEU, R. C. F. F.
700 1 $aSILVA, R. G. da
700 1 $aLOPES, J. W. B.
700 1 $aBEZERRA, F. M. L.
773 $tRevista Brasileira de Zootecnia$gv. 40, n. 8, p. 1629-1637, 2011.
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Embrapa Caprinos e Ovinos (CNPC) |
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