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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/08/2023 |
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Data da última atualização: |
21/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DAIBERT, R. M. de P.; BIAGI JUNIOR, C. A. O. de; VIEIRA, F. de O.; SILVA, M. V. G. B.; HOTTZ, E. D.; PINHEIRO, M. B. M.; REIS, D. R. de L.; PEREIRA, H. P.; MARTINS, M. F.; BRANDAO, H. de M.; MACHADO, M. A.; CARVALHO, W. A. |
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Afiliação: |
RAQUEL MORAIS DE PAIVA DAIBERT, Universidade Federal de Juiz de Fora; CARLOS ALBERTO OLIVEIRA DE BIAGI JUNIOR, Universidade de São Paulo; FELIPE DE OLIVEIRA VIEIRA, Universidade Federal de Juiz de Fora; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; EUGENIO DAMACENO HOTTZ, Universidade Federal de Juiz de Fora; MARIANA BRANDI MENDONÇA PINHEIRO, Universidade Federal de Juiz de Fora; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; HYAGO PASSE PEREIRA, Universidade Federal de Juiz de Fora; MARTA FONSECA MARTINS, CNPGL; HUMBERTO DE MELLO BRANDAO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL. |
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Título: |
Lipopolysaccharide triggers different transcriptional signatures in taurine and indicine cattle macrophages: Reactive oxygen species and potential outcomes to the development of immune response to infections. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
PLoS One, v. 15, n. 11, e0241861, 2020. |
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DOI: |
https://doi.org/10.1371%2Fjournal.pone.0241861 |
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Idioma: |
Inglês |
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Conteúdo: |
Macrophages are classified upon activation as classical activated M1 and M2 anti-inflammatory regulatory populations. This macrophage polarization is well characterized in humans and mice, but M1/M2 profile in cattle has been far less explored. Bos primigenius taurus (taurine) and Bos primigenius indicus (indicine) cattle display contrasting levels of resistance to infection and parasitic diseases such as C57BL/6J and Balb/c murine experimental models of parasite infection outcomes based on genetic background. Thus, we investigated the differential gene expression profile of unstimulated and LPS stimulated monocyte-derived macrophages (MDMs) from Holstein (taurine) and Gir (indicine) breeds using RNA sequencing methodology. For unstimulated MDMs, the contrast between Holstein and Gir breeds identified 163 Differentially Expressed Genes (DEGs) highlighting the higher expression of C-C chemokine receptor type five (CCR5) and BOLA-DQ genes in Gir animals. LPS-stimulated MDMs from Gir and Holstein animals displayed 1,257 DEGs enriched for cell adhesion and inflammatory responses. Gir MDMs cells displayed a higher expression of M1 related genes like Nitric Oxide Synthase 2 (NOS2), Toll like receptor 4 (TLR4), Nuclear factor NF-kappa-B 2 (NFKB2) in addition to higher levels of transcripts for proinflammatory cytokines, chemokines, complement factors and the acute phase protein Serum Amyloid A (SAA). We also showed that gene expression of inflammatory M1 population markers, complement and SAA genes was higher in Gir in buffy coat peripheral cells in addition to nitric oxide concentration in MDMs supernatant and animal serum. Co-expression analyses revealed that Holstein and Gir animals showed different transcriptional signatures in the MDMs response to LPS that impact on cell cycle regulation, leukocyte migration and extracellular matrix organization biological processes. Overall, the results suggest that Gir animals show a natural propensity to generate a more pronounced M1 inflammatory response than Holstein, which might account for a faster immune response favouring resistance to many infection diseases. MenosMacrophages are classified upon activation as classical activated M1 and M2 anti-inflammatory regulatory populations. This macrophage polarization is well characterized in humans and mice, but M1/M2 profile in cattle has been far less explored. Bos primigenius taurus (taurine) and Bos primigenius indicus (indicine) cattle display contrasting levels of resistance to infection and parasitic diseases such as C57BL/6J and Balb/c murine experimental models of parasite infection outcomes based on genetic background. Thus, we investigated the differential gene expression profile of unstimulated and LPS stimulated monocyte-derived macrophages (MDMs) from Holstein (taurine) and Gir (indicine) breeds using RNA sequencing methodology. For unstimulated MDMs, the contrast between Holstein and Gir breeds identified 163 Differentially Expressed Genes (DEGs) highlighting the higher expression of C-C chemokine receptor type five (CCR5) and BOLA-DQ genes in Gir animals. LPS-stimulated MDMs from Gir and Holstein animals displayed 1,257 DEGs enriched for cell adhesion and inflammatory responses. Gir MDMs cells displayed a higher expression of M1 related genes like Nitric Oxide Synthase 2 (NOS2), Toll like receptor 4 (TLR4), Nuclear factor NF-kappa-B 2 (NFKB2) in addition to higher levels of transcripts for proinflammatory cytokines, chemokines, complement factors and the acute phase protein Serum Amyloid A (SAA). We also showed that gene expression of inflammatory M1 population markers, complem... Mostrar Tudo |
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Palavras-Chave: |
Doença parasitária; Expressão gênica; Lipopolissacarídeo; Macrófago. |
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Thesagro: |
Bovino; Doença Animal; Gado Gir; Gado Holandês; Infecção. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156010/1/Lipopolysaccharide-triggers-different-transcriptional-signatures-in-taurine.pdf
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Marc: |
LEADER 03345naa a2200373 a 4500
001 2156010
005 2023-08-21
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371%2Fjournal.pone.0241861$2DOI
100 1 $aDAIBERT, R. M. de P.
245 $aLipopolysaccharide triggers different transcriptional signatures in taurine and indicine cattle macrophages$bReactive oxygen species and potential outcomes to the development of immune response to infections.$h[electronic resource]
260 $c2020
520 $aMacrophages are classified upon activation as classical activated M1 and M2 anti-inflammatory regulatory populations. This macrophage polarization is well characterized in humans and mice, but M1/M2 profile in cattle has been far less explored. Bos primigenius taurus (taurine) and Bos primigenius indicus (indicine) cattle display contrasting levels of resistance to infection and parasitic diseases such as C57BL/6J and Balb/c murine experimental models of parasite infection outcomes based on genetic background. Thus, we investigated the differential gene expression profile of unstimulated and LPS stimulated monocyte-derived macrophages (MDMs) from Holstein (taurine) and Gir (indicine) breeds using RNA sequencing methodology. For unstimulated MDMs, the contrast between Holstein and Gir breeds identified 163 Differentially Expressed Genes (DEGs) highlighting the higher expression of C-C chemokine receptor type five (CCR5) and BOLA-DQ genes in Gir animals. LPS-stimulated MDMs from Gir and Holstein animals displayed 1,257 DEGs enriched for cell adhesion and inflammatory responses. Gir MDMs cells displayed a higher expression of M1 related genes like Nitric Oxide Synthase 2 (NOS2), Toll like receptor 4 (TLR4), Nuclear factor NF-kappa-B 2 (NFKB2) in addition to higher levels of transcripts for proinflammatory cytokines, chemokines, complement factors and the acute phase protein Serum Amyloid A (SAA). We also showed that gene expression of inflammatory M1 population markers, complement and SAA genes was higher in Gir in buffy coat peripheral cells in addition to nitric oxide concentration in MDMs supernatant and animal serum. Co-expression analyses revealed that Holstein and Gir animals showed different transcriptional signatures in the MDMs response to LPS that impact on cell cycle regulation, leukocyte migration and extracellular matrix organization biological processes. Overall, the results suggest that Gir animals show a natural propensity to generate a more pronounced M1 inflammatory response than Holstein, which might account for a faster immune response favouring resistance to many infection diseases.
650 $aBovino
650 $aDoença Animal
650 $aGado Gir
650 $aGado Holandês
650 $aInfecção
653 $aDoença parasitária
653 $aExpressão gênica
653 $aLipopolissacarídeo
653 $aMacrófago
700 1 $aBIAGI JUNIOR, C. A. O. de
700 1 $aVIEIRA, F. de O.
700 1 $aSILVA, M. V. G. B.
700 1 $aHOTTZ, E. D.
700 1 $aPINHEIRO, M. B. M.
700 1 $aREIS, D. R. de L.
700 1 $aPEREIRA, H. P.
700 1 $aMARTINS, M. F.
700 1 $aBRANDAO, H. de M.
700 1 $aMACHADO, M. A.
700 1 $aCARVALHO, W. A.
773 $tPLoS One$gv. 15, n. 11, e0241861, 2020.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 26 | |
| 21. |  | JENS, S. M.; NOGUEIRA, A. L.; MAGALHÃES, R. L. O. de; FRACETTI, M. E. M.; PEREIRA, H. P.; DOMINGUES, R.; REIS, D. R. de L.; PANETTO, J. C. do C.; SILVA, M. V. G. B.; MACHADO, M. A.; MARTINS, M. F. Identificação de portadores da deficiência do Fator XI em touros das Raças Gir e Holandesa. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE PIBIC/CNPQ, 26., 2022, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2022. p. 71-74.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 22. | | JENS, S. M.; NOGUEIRA, A. L.; MAGALHÃES, R. L. O. de; FRACETTI, M. E. M.; PEREIRA, H. P.; DOMINGUES, R.; REIS, D. R. de L.; PANETTO, J. C. do C.; SILVA, M. V. G. B.; MACHADO, M. A.; MARTINS, M. F. Identificação de portadores da deficiência do Fator XI em touros das Raças Gir e Holandesa. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE PIBIC/CNPQ, 26., 2022, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2022. p. 71-74.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
|  |
| 23. | | NOGUEIRA, A. L.; MAGALHÃES, R. L. O. de; JENS, S. M.; FRACETTI, M. E. M.; PEREIRA, H. P.; DOMINGUES, R.; REIS, D. R. de L.; PANETTO, J. C. do C.; SILVA, M. V. G. B.; MARTINS, M. F.; MACHADO, M. A. Identificação de portadores da síndrome de Brachyspina em touros das raças Gir e Holandesa. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE PIBIC/CNPQ, 26., 2022, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2022. p. 66-70.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 24. | | NOGUEIRA, A. L.; FERREIRA, R. C.; LIMA, L. L.; MAGALHÃES, R. L. O. de; CARVALHO, C. V. de; HONÓRIO, N. T. de B. S.; PEREIRA, H. P.; DOMINGUES, R.; REIS, D. R. de L.; PANETTO, J. C. do C.; SILVA, M. V. G. B.; MARTINS, M. F.; MACHADO, M. A. Identificação de portadores das síndromes Brachyspina e deficiência do Fator XI em touros da raça Girolando. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 27., 2023, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2024. p. 82-87. (Embrapa Gado de Leite. Eventos Técnicos & Científicos, 2). Pibic/CNPq.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 25. | | LIMA, T. R. de; MALUF, V. H. H. K.; CAMPOS, R. A.; SOARES, I. K. de A.; PEREIRA, H. P.; DOMINGUES, R.; REIS, D. R. de L.; MARTINS, M. F.; PANETTO, J. C. do C.; SILVA, M. V. G. B.; MACHADO, M. A. Pesquisa do alelo B de beta-caseína em touros da raça Girolando por tetraprimer ARMS-PCR1. In: PASSOS, L. P. (ed.). Coletânea de Iniciação Científica da Embrapa Gado de Leite-PIBIC CNPq 2020-2021. Juiz de Fora: Embrapa Gado de Leite, 2021. (Embrapa Gado de Leite. Documentos, 262).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 26. | | SOARES, I. K. de A.; CUNHA FILHO, J. A. da; SOARES, G. O.; LIMA, T. R. de; MALUF, V. H. H. K.; CAMPOS, R. A.; LOURES, C. de O.; DOMINGUES, R.; GASPAR, E. B.; REIS, D. R. de L.; CAMPOS, M. M.; PEREIRA, H. P.; MACHADO, M. A.; MARTINS, M. F.; CARVALHO, W. A. Otimização de metodologia para isolamento de leucócitos a partir de sangue total periférico de bovinos. In: PASSOS, L. P. (ed.). Coletânea de Iniciação Científica da Embrapa Gado de Leite-PIBIC CNPq 2020-2021. Juiz de Fora: Embrapa Gado de Leite, 2021. (Embrapa Gado de Leite. Documentos, 262).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 26 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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