Data/hora: 07/06/2024 04:32:42

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 31/08/2015
Data da última atualização: | 25/05/2017
Autoria: | PEREIRA, G. da S.; CAZÉ, A. L. R.; SILVA, M. G. da; ALMEIDA, V. C.; MAGALHAES, F. O. da C.; SILVA FILHO, J. L. da; BARROSO, P. A. V.; HOFFMANN, L. V.
Afiliação: | GULHERME DA SILVA PEREIRA, Universidade Estadual da Paraíba; ANA LUÍZA RAMOS CAZÉ, Universidade Estadual da Paraíba; MICHELLE GARCIA DA SILVA, Universidade Estadual da Paraíba; VANESSA CAVALCANTE ALMEIDA, Universidade Estadual da Paraíba; FERNANDA OLIVEIRA DA C MAGALHAES, CNPA; JOAO LUIS DA SILVA FILHO, CNPA; PAULO AUGUSTO VIANNA BARROSO, CNPA; LUCIA VIEIRA HOFFMANN, CNPA.
Título: | Optimal use of SSR markers for varietal identification of upland cotton.
Ano de publicação: | 2015
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 50, n. 7, p. 571-581, jul. 2015.
Idioma: | Inglês
Notas: | Título em português: Uso otimizado de marcadores SSR para identificação varietal de algodoeiro herbáceo.
Conteúdo: | The objective of this work was to identify polymorphic simple sequence repeat (SSR) markers for varietal identification of cotton and evaluation of the genetic distance among the varieties. Initially, 92 SSR markers were genotyped in 20 Brazilian cotton cultivars. Of this total, 38 loci were polymorphic, two of which were amplified by one primer pair; the mean number of alleles per locus was 2.2. The values of polymorphic information content (PIC) and discrimination power (DP) were, on average, 0.374 and 0.433, respectively. The mean genetic distance was 0.397 (minimum of 0.092 and maximum of 0.641). A panel of 96 varieties originating from different regions of the world was assessed by 21 polymorphic loci derived from 17 selected primer pairs. Among these varieties, the mean genetic distance was 0.387 (minimum of 0 and maximum of 0.786). The dendrograms generated by the unweighted pair group method with arithmetic average (UPGMA) did not reflect the regions of Brazil (20 genotypes) or around the world (96 genotypes), where the varieties or lines were selected. Bootstrap resampling shows that genotype identification is viable with 19 loci. The polymorphic markers evaluated are useful to perform varietal identification in a large panel of cotton varieties and may be applied in studies of the species diversity.
Palavras-Chave: | Cultivar discrimination; Intraspecific polymorhism; Microsatellite; Microssatélite; Polimorfismo intraespecífico.
Thesagro: | Gossypium Hirsutum.
Thesaurus Nal: | Microsatellite repeats.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/128830/1/Optimal-use-of-SSR-markers.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (58132-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS