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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
08/03/2016 |
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Data da última atualização: |
10/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
YUYAMA, P. M.; REIS JUNIOR, O.; IVAMOTO, S. T.; DOMINGUES, D. S.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; CHARMETANT, P.; LEROY, T.; PEREIRA, L. F. P. |
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Afiliação: |
PRISCILA MARY YUYAMA, UEL; OSVALDO REIS JÚNIOR, UNICAMP; SUZANA TIEMI IVAMOTO, UEL/IAPAR; DOUGLAS SILVA DOMINGUES, IAPAR/UNESP; MARCELO FALSARELLA CARAZZOLLE, UNICAMP; GONÇALO AMARANTE GUIMARÃES PEREIRA, UNICAMP; PIERRE CHARMETANT, CIRAD; THIERRY LEROY, CIRAD; LUIZ FILIPE PROTASIO PEREIRA, SAPC. |
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Título: |
Transcriptome analysis in Coffea eugenioides, an Arabica coffee ancestor, reveals differentially expressed genes inleaves and fruits. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Molecular Genetics and Genomics, v. 291, n. 1, p. 323-336. 2016 |
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Idioma: |
Inglês |
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Conteúdo: |
Studies in diploid parental species of polyploid plants are important to understand their contributions to the formation of plant and species evolution. Coffea eugenioides is a diploid species that is considered to be an ancestor of allopolyploid Coffea arabica together with Coffea canephora. Despite its importance in the evolutionary history of the main economic species of coffee, no study has focused on C. eugenioides molecular genetics. RNA-seq creates the possibility to generate reference transcriptomes and identify coding genes and potential candidates related to important agronomic traits. Therefore, the main objectives were to obtain a global overview of transcriptionally active genes in this species using next generation sequencing and to analyze specific genes that were highly expressed in leaves and fruits with potential exploratory characteristics for breeding and understanding the evolutionary biology of coffee. A de novo assembly generated 36,935 contigs that were annotated using eight databases. We observed a total of ~5000 differentially expressed genes between leaves and fruits. Several genes exclusively expressed in fruits did not exhibit similarities with sequences in any database. We selected ten differentially expressed unigenes in leaves and fruits to evaluate transcriptional profiles using qPCR. Our study provides the first gene catalog for C. eugenioides and enhances the knowledge concerning the mechanisms involved in the C. arabica homeologous. Furthermore, this work will open new avenues for studies into specific genes and pathways in this species, especially related to fruit, and our data have potential value in assisted breeding applications. MenosStudies in diploid parental species of polyploid plants are important to understand their contributions to the formation of plant and species evolution. Coffea eugenioides is a diploid species that is considered to be an ancestor of allopolyploid Coffea arabica together with Coffea canephora. Despite its importance in the evolutionary history of the main economic species of coffee, no study has focused on C. eugenioides molecular genetics. RNA-seq creates the possibility to generate reference transcriptomes and identify coding genes and potential candidates related to important agronomic traits. Therefore, the main objectives were to obtain a global overview of transcriptionally active genes in this species using next generation sequencing and to analyze specific genes that were highly expressed in leaves and fruits with potential exploratory characteristics for breeding and understanding the evolutionary biology of coffee. A de novo assembly generated 36,935 contigs that were annotated using eight databases. We observed a total of ~5000 differentially expressed genes between leaves and fruits. Several genes exclusively expressed in fruits did not exhibit similarities with sequences in any database. We selected ten differentially expressed unigenes in leaves and fruits to evaluate transcriptional profiles using qPCR. Our study provides the first gene catalog for C. eugenioides and enhances the knowledge concerning the mechanisms involved in the C. arabica homeologous. Furthe... Mostrar Tudo |
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Palavras-Chave: |
Differentially expressed genes; Gene annotation; Homeologous; RNA-seq. |
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Thesaurus Nal: |
Coffea. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140757/1/Transcriptome-analysis-in-coffea.pdf
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Marc: |
LEADER 02549naa a2200277 a 4500
001 2040005
005 2016-03-10
008 2016 bl uuuu u00u1 u #d
100 1 $aYUYAMA, P. M.
245 $aTranscriptome analysis in Coffea eugenioides, an Arabica coffee ancestor, reveals differentially expressed genes inleaves and fruits.$h[electronic resource]
260 $c2016
520 $aStudies in diploid parental species of polyploid plants are important to understand their contributions to the formation of plant and species evolution. Coffea eugenioides is a diploid species that is considered to be an ancestor of allopolyploid Coffea arabica together with Coffea canephora. Despite its importance in the evolutionary history of the main economic species of coffee, no study has focused on C. eugenioides molecular genetics. RNA-seq creates the possibility to generate reference transcriptomes and identify coding genes and potential candidates related to important agronomic traits. Therefore, the main objectives were to obtain a global overview of transcriptionally active genes in this species using next generation sequencing and to analyze specific genes that were highly expressed in leaves and fruits with potential exploratory characteristics for breeding and understanding the evolutionary biology of coffee. A de novo assembly generated 36,935 contigs that were annotated using eight databases. We observed a total of ~5000 differentially expressed genes between leaves and fruits. Several genes exclusively expressed in fruits did not exhibit similarities with sequences in any database. We selected ten differentially expressed unigenes in leaves and fruits to evaluate transcriptional profiles using qPCR. Our study provides the first gene catalog for C. eugenioides and enhances the knowledge concerning the mechanisms involved in the C. arabica homeologous. Furthermore, this work will open new avenues for studies into specific genes and pathways in this species, especially related to fruit, and our data have potential value in assisted breeding applications.
650 $aCoffea
653 $aDifferentially expressed genes
653 $aGene annotation
653 $aHomeologous
653 $aRNA-seq
700 1 $aREIS JUNIOR, O.
700 1 $aIVAMOTO, S. T.
700 1 $aDOMINGUES, D. S.
700 1 $aCARAZZOLLE, M. F.
700 1 $aPEREIRA, G. A. G.
700 1 $aCHARMETANT, P.
700 1 $aLEROY, T.
700 1 $aPEREIRA, L. F. P.
773 $tMolecular Genetics and Genomics$gv. 291, n. 1, p. 323-336. 2016
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 45 | |
| 1. |  | LOURENÇO, R. T.; CUNHA, A. F.; TSUKUMO, F.; PEREIRA, G. A. G.; GRATTAPAGLIA, D. Análise da composição e organização do genoma de Eucalyptus grandis com base em sequenciamento "shotgun" por amostragem ("sample sequencing"). In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2002. p. 30.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | ANDRADE, A. C.; VIEIRA, L. G. E.; COLOMBO, C. A.; PEREIRA, G. A. G. Coffee functional genomics in Brazil. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2006.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | ANDRADE, A. C.; VIEIRA, L. G. E.; COLOMBO, C. A.; PEREIRA, G. A. G. Coffee functional genomics in Brazil. INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | LOURENÇO, R. T.; PAPPAS, G. J.; CUNHA, A. F.; PEREIRA, G. A. G.; GRATTAPAGLIA, D. Estrutura genômica de três megabases de dna genômico "shotgun" de eucalyptus: conteúdo nucleotídico, seouências repetitivas e genes. ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENETICOS E BIOTECNOLOGIA, 8., 2003, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2003. p. 74| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | BATISTA, A. R. S.; LOURENÇO, R. T.; PEREIRA, G. A. G.; GRATTAPAGLIA, D. Novos microssatélltes para eucalyptus a partir de clones "shotgun". ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENETICOS E BIOTECNOLOGIA, 8., 2003, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2003. p. 79| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | NASCIMENTO, L. C. do; COSTA, G. G. L.; BINNECK, E.; PEREIRA, G. A. G.; CARAZZOLLE, M. F. A web-based bioinformatics interface applied to the GENOSOJA Project: databases and pipelines. Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 203-211, May 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | IVAMOTO, S. T.; REIS JUNIOR, O.; SAKURAY, L. M.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; PEREIRA, L. F. P. Transcriptome analysis in leaves, flowers and initial fruit development of Coffea arabica L. In: PLANT & ANIMAL GENOME CONFERENCE INTERNATIONAL, 23., 2015, San Diego, CA. Poster..., 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 10. | | SALAZAR, M. S.; CAMARGO, E. L. O.; DECKMANN, A. C.; CARAZZOLLE, M. F.; LEPIKSON NETO, J.; MEDRANO, F. J.; GRATTAPAGLIA, D.; PEREIRA, G. A. G. Análise do genoma de espécies de eucalipto visando a identificação de genes/metabolismos chave para o incremento da sua produtividade para orientar o seu melhoramento. In: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Resumos... Salvador: SBG, 2008. p. 240. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | LOPES, F. R.; ANDRADE, A. C.; MARRACCINI, P.; TEIXEIRA, J. B.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; CARARETO, C. M. A. Evolutionary changes of the gene expression pattern mediated by exonized transposable element sequences in coffee genomes. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2010, Bali. Preceedings... [S.l]: Association for Science and Information on Coee (ASIC), 2010. p. 775-779| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | ROJAS, T. C. G.; MALUTA, R. P.; PARIZZI, L. P.; KOENIGKAN, L. V.; YANG, J.; YU, J.; PEREIRA, G. A. G.; SILVEIRA, W. D. da. Genome sequences of avian pathogenic Escherichia coli strains isolated from Brazilian commercial poultry. Genome Announcements, v. 1, n. 2, p. 1-2, Mar./Apr. 2013. Article e00110-13.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 13. | | SOARES-CAVALCANTI, N. M.; BELARMINO, L. C.; KIDO, E. A.; PANDOLFI, V.; MARCELINO-GUIMARÃES, F. C.; RODRIGUES, F. A.; PEREIRA, G. A. G.; BENKO-ISEPPON, A. M. Overall picture of expressed Heat Shock Factors in Glycine max, Lotus japonicus and Medicago truncatula. Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 247-259, May 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 14. | | VIDAL, R.; LEROY, L.; BELLIS, F. de; POT, D.; RODRIGUES, G. C.; PEREIRA, G. A. G.; ANDRADE, A. C.; MARRACCINI, P. High-throughput sequencing of cDNA extracted from meristems of Coffea arabica cv. Rubi and Iapar59 submitted to different water field conditions. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2010, Bali. Preceedings... [S.l]: Association for Science and Information on Coee (ASIC), 2010. p. 708-712| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | VIDAL, R. O.; ALEKCEVETCH, J. C.; LEROY, T.; DE BELLIS, F.; POT, D.; RODRIGUES, G. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; ANDRADE, A. C.; MARRACCINI, P. High-through put sequencing of CDNA shows that CV. Rubi and IAPAR59 of Coffea Arabica have different molecular response to water privation. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2012, San José. Proceedings... [S.l]: Association for Science and Information on Coffee (ASIC), 2012. p. 61.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | YUYAMA, P. M.; REIS JUNIOR, O.; IVAMOTO, S. T.; DOMINGUES, D. S.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; CHARMETANT, P.; LEROY, T.; PEREIRA, L. F. P. Transcriptome analysis in Coffea eugenioides, an Arabica coffee ancestor, reveals differentially expressed genes inleaves and fruits. Molecular Genetics and Genomics, v. 291, n. 1, p. 323-336. 2016| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
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| 17. | | CARAZZOLLE, M. F.; COSTA, G. G. L.; HERAI, R. H.; R. JÚNIOR, O.; NASCIMENTO, L. C.; TEIXEIRA, P. J.; TIBURCIO, R. A.; MONDEGO, J. M. C.; PEREIRA, G. A. G. The genome projects of the closely related cacao pathogens Moniliophthora roreri and Moniliophthora perniciosa. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 6., 2010, Ouro Preto. Abstracts... [S.l.: s.n.], 2010. p. 22. X-meeting 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 18. | | NASCIMENTO, L. C.; VIDAL R. O.; MONDEGO, J. M. C.; COSTA, G. G. L.; JUNIOR, O. R.; RODRIGUES, F.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V.; PEREIRA, G. A. G.; CARAZZOLLE, M. F. Genotipagem de cultivares brasileiros de soja através da detecção de SNPs. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: resumos. Brasília, DF: Embrapa, 2012. p. 15, res. 8.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 19. | | NASCIMENTO, L. C.; VIDAL R. O.; MONDEGO, J. M. C.; COSTA, G. G. L.; JUNIOR, O. R.; RODRIGUES, F.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V.; PEREIRA, G. A. G.; CARAZZOLLE, M. F. Genotipagem de cultivares brasileiros de soja através da detecção de SNPs. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa, 2012. 4 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 20. | | NASCIMENTO, L. C.; COSTA, G. G. L.; VIDAL, R. O.; MEYER, L.; BINNECK, E.; KIDO, E. A.; RODRIGUES, F.; KULCHESKI, F. R.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; PEREIRA, G. A. G.; CARAZZOLLE, M. F. Bioinformatics analysis applied to genosoja project. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 5., 2009, Angra dos Reis. Abstracts book ... Rio de Janeiro: AB3C, 2009. p. 22. X-Meeting 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 45 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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