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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
14/02/2019 |
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Data da última atualização: |
02/08/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LOPES, M. T. do R.; PEREIRA, L. A.; MORAES, J. I. da S.; SOUZA, B. de A.; PEREIRA, F. de M. |
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Afiliação: |
MARIA TERESA DO REGO LOPES, CPAMN; LEUDIMAR AIRES PEREIRA, UFPI; JOSEANE INÁCIO DA SILVA MORAES; BRUNO DE ALMEIDA SOUZA, CPAMN; FABIA DE MELLO PEREIRA, CPAMN. |
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Título: |
Caracterização de Arrabidae Chica (Humb. & Bonpl.) Verlot como planta melífera em região de transição caatinga-cerrado no Estado do Piauí. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 4., 2016, Curitiba. Recursos genéticos no Brasil: a base para o desenvolvimento sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2016. |
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Páginas: |
p. 253. |
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Idioma: |
Português |
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Conteúdo: |
As abelhas são importantes polinizadores da flora nativa e encontram-se ameaçadas devido às ações antrópicas. O conhecimento e caracterização da flora utilizada como fonte de alimento pelas abelhas é fundamental para a conservação dos recursos genéticos destes insetos. A espécie Arrabidae chica(Humb. & Bonpl.) Verlot (Bignoniaceae), conhecida popularmente como crajiru, pariri ou chica, é uma trepadeira lenhosa nativa de quase todo o Brasil, utilizada como planta medicinal, especialmente nas regiões Norte e Nordeste. |
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Palavras-Chave: |
Abelhas. |
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Thesagro: |
Conservação; Néctar. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192838/1/Anais-IV-CBRG2016-00253.pdf
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Marc: |
LEADER 01362nam a2200205 a 4500
001 2106034
005 2019-08-02
008 2016 bl uuuu u00u1 u #d
100 1 $aLOPES, M. T. do R.
245 $aCaracterização de Arrabidae Chica (Humb. & Bonpl.) Verlot como planta melífera em região de transição caatinga-cerrado no Estado do Piauí.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 4., 2016, Curitiba. Recursos genéticos no Brasil: a base para o desenvolvimento sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos$c2016
300 $ap. 253.
520 $aAs abelhas são importantes polinizadores da flora nativa e encontram-se ameaçadas devido às ações antrópicas. O conhecimento e caracterização da flora utilizada como fonte de alimento pelas abelhas é fundamental para a conservação dos recursos genéticos destes insetos. A espécie Arrabidae chica(Humb. & Bonpl.) Verlot (Bignoniaceae), conhecida popularmente como crajiru, pariri ou chica, é uma trepadeira lenhosa nativa de quase todo o Brasil, utilizada como planta medicinal, especialmente nas regiões Norte e Nordeste.
650 $aConservação
650 $aNéctar
653 $aAbelhas
700 1 $aPEREIRA, L. A.
700 1 $aMORAES, J. I. da S.
700 1 $aSOUZA, B. de A.
700 1 $aPEREIRA, F. de M.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão; Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
15/09/2022 |
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Data da última atualização: |
16/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FERREIRA, A. L.; FARIA, J. C. de; MOURA, M. da C.; ZAIDEM, A. L. de M.; PIZETTA, C. S. R.; FREITAS, E. de O.; COELHO, G. R. C.; SILVA, J. F. A. e; BARRIGOSSI, J. A. F.; HOFFMANN, L. V.; SOUZA, T. L. P. O. de; ARAGÃO, F. J. L.; PINHEIRO, P. V. |
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Afiliação: |
AMANDA LOPES FERREIRA, bolsista CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MATHEUS DA COSTA MOURA, UNIVERSIDADE FEDERAL DE GOIÁS; ANTÔNIA LOPES DE MENDONÇA ZAIDEM, UNIVERSIDADE FEDERAL DE GOIÁS; CAROLINA SENHORINHO RAMALHO PIZETTA; ELÍNEA DE OLIVEIRA FREITAS; GESIMARIA RIBEIRO COSTA COELHO, CNPAF; JOSE FRANCISCO ARRUDA E SILVA, CNPAF; JOSE ALEXANDRE F BARRIGOSSI, CNPAF; LUCIA VIEIRA HOFFMANN, CNPA; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen; PATRICIA VALLE PINHEIRO, CNPAF. |
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Título: |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 13, 984804, Aug. 2022. |
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ISSN: |
1664-462X |
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DOI: |
https://doi.org/10.3389/fpls.2022.984804 |
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Idioma: |
Inglês |
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Conteúdo: |
Common bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases. MenosCommon bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays we... Mostrar Tudo |
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Palavras-Chave: |
Dry bean; Insect pest management; VATPase. |
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Thesagro: |
Bemisia Tabaci; Feijão; Mosca Branca; Phaseolus Vulgaris; Planta Transgênica. |
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Thesaurus NAL: |
Beans; RNA interference; Transgenic plants. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146488/1/fpls-984804.pdf
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Marc: |
LEADER 03529naa a2200421 a 4500
001 2146488
005 2022-09-16
008 2022 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $ahttps://doi.org/10.3389/fpls.2022.984804$2DOI
100 1 $aFERREIRA, A. L.
245 $aWhitefly-tolerant transgenic common bean (Phaseolus vulgaris) line.$h[electronic resource]
260 $c2022
520 $aCommon bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases.
650 $aBeans
650 $aRNA interference
650 $aTransgenic plants
650 $aBemisia Tabaci
650 $aFeijão
650 $aMosca Branca
650 $aPhaseolus Vulgaris
650 $aPlanta Transgênica
653 $aDry bean
653 $aInsect pest management
653 $aVATPase
700 1 $aFARIA, J. C. de
700 1 $aMOURA, M. da C.
700 1 $aZAIDEM, A. L. de M.
700 1 $aPIZETTA, C. S. R.
700 1 $aFREITAS, E. de O.
700 1 $aCOELHO, G. R. C.
700 1 $aSILVA, J. F. A. e
700 1 $aBARRIGOSSI, J. A. F.
700 1 $aHOFFMANN, L. V.
700 1 $aSOUZA, T. L. P. O. de
700 1 $aARAGÃO, F. J. L.
700 1 $aPINHEIRO, P. V.
773 $tFrontiers in Plant Science$gv. 13, 984804, Aug. 2022.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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