Data/hora: 11/06/2024 14:34:28

Biblioteca(s): 	Embrapa Arroz e Feijão.	
Data corrente: 	06/10/2004	
Data da última atualização: 	14/10/2013	
Autoria: 	TEIXEIRA-CABRAL, T. A.; SAKIYAMA, N. S.; ZAMBOLIM, L.; PEREIRA, A. A.; BARROS, E. G.; SAKIYAMA, C. C. H.	
Título: 	Reproducibility of the RAPD marker and its efficiency in coffee tree genotype grouping analysis.	
Ano de publicação: 	2002	
Fonte/Imprenta: 	Crop Breeding and Applied Biotechnology, Londrina, v. 2, n. 1, p. 121-129, Mar. 2002.	
Idioma: 	Inglês	
Conteúdo: 	The genetic diversity of Coffea arabica L. cultivars is relatively narrow and its assessment and increase is important for breeding. Fifty two arbitrary primers were used to evaluate the reproducibility and the influence of the number of RAPD (Random Amplified Polymorphic DNA) markers on the estimation of genetic distances among 40 genotypes of Coffea spp. The average number of polymorphic bands was 6.69 per primer among all genotypes, and 1.27 among arabica coffee genotypes. RAPD markers were efficient in estimating the genetic distances among the genotypes. The increase in RAPD loci number during grouping analysis did not affect the major groups' composition; however, it affected the composition of subgroups. Marker reproducibility was 76.88% and replicated data was recommended for distinguishing genotypes with the same genetic background.	
Palavras-Chave: 	Coffee breeding; Diversidade genética; Diversity; DNA markers; Melhoramento genético.	
Thesagro: 	Café; Coffea Arábica.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (21687-1) - ADD - AP - -- - 631.05 - C - 2004.00061 - DIS