Data/hora: 12/06/2024 00:55:30

Biblioteca(s): 	Embrapa Cerrados.	
Data corrente: 	16/12/2013	
Data da última atualização: 	10/12/2014	
Autoria: 	PEREIRA, G. S.; NUNES, E. S.; LAPERUTA, L. D. C.; BRAGA, M. F.; PENHA, H. A.; DINIZ, A. L.; MUNHOZ, C. F.; GAZAFFI, R.; GARCIA, A. A. F.; VIEIRA, M. L. C.	
Afiliação: 	ESALQ/USP; ESALQ/USP; ESALQ/USP; MARCELO FIDELES BRAGA, CPAC; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP.	
Título: 	Molecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species.	
Ano de publicação: 	2013	
Fonte/Imprenta: 	Annals of Applied Biology, v. 162, n. 3, p. 347-361, 2013.	
ISSN: 	0003-4746	
DOI: 	10.1111/aab.12028	
Idioma: 	Inglês	
Conteúdo: 	One of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n&#8201;=&#8201;18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87&#8201;bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score&#8201;&#8805;&#8201;5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of which fit a segregation ratio of 1:1:1:1, 1.3% a ratio of 1:2:1, 27.3% a ratio of 3:1 and 69.4% a ratio of 1:1. The map spanned a total of 2073.0&#8201;cM, with an average distance between adjacent markers of 3.8&#8201;cM. This is the first linkage study on sweet passion fruit and should prove useful for quantitative trait loci mapping.	
Thesagro: 	Maracujá; Marcador genético; Marcador molecular; Melhoramento genético vegetal; Polimorfismo Genético.	
Thesaurus Nal: 	Genetic improvement; Genetic markers; Genetic polymorphism; Passiflora alata; Passion fruits.	
Categoria do assunto: 	G Melhoramento Genético	
Registro original: 	Embrapa Cerrados (CPAC)	
Exemplares: 	CPAC - (33762-1) - UPC - AP - PP - S1633 -  - S1633 - DIS