Data/hora: 03/06/2024 14:12:55

Biblioteca(s): | Embrapa Arroz e Feijão; Embrapa Solos.
Data corrente: | 06/02/2015
Data da última atualização: | 06/02/2015
Autoria: | LEITE, D. C. A.; BALIEIRO, F. C.; C. A. PIRES; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. C.; PEIXOTO, R. S.
Afiliação: | D. C. A. LEITE, UFRJ; FABIANO DE CARVALHO BALIEIRO, CNPS; C. A. PIRES; BEATA EMOKE MADARI, CNPAF; A. S. ROSADO, UFRJ; HEITOR LUIZ DA COSTA COUTINHO, CNPS; R. S. PEIXOTO, UFRJ.
Título: | Comparison of DNA extraction protocols for microbial communities from soil treated with biochar.
Ano de publicação: | 2014
Fonte/Imprenta: | Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 175-183, 2014.
DOI: | 10.1590/S1517-83822014000100023
Idioma: | Inglês
Conteúdo: | Many studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-biochar samples.
Palavras-Chave: | Comunidades microbianas; Extração de DNA; Índice de pureza do DNA; PCR-DGGE.
Thesaurus Nal: | biochar.
Categoria do assunto: | P Recursos Naturais, Ciências Ambientais e da Terra
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/117433/1/BJMv45n1a23.pdf
Registro original: | Embrapa Solos (CNPS)

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CNPS - (18804-1) - UPC - AP - DD -  -  - 2015.00035 - DIS
CNPAF - (33178-1) - UPC - AP - DD - 2014.086 -  - 2014.086 - DIS