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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
09/12/2021 |
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Data da última atualização: |
10/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEIXOTO, R. M.; SOUSA, A. L. M. de; ARAÚJO, J. F.; PINHEIRO, R. R. |
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Afiliação: |
RENATO MESQUITA PEIXOTO; ANA LÍDIA MADEIRA DE SOUSA; JUSCILÂNIA FURTADO ARAÚJO; RAYMUNDO RIZALDO PINHEIRO, CNPC. |
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Título: |
Western Blot no imunodiagnóstico de lentivírus de pequenos ruminantes. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 49, pub. 1781, p. 1-11, 2021. |
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Idioma: |
Português |
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Conteúdo: |
Background: Small ruminant lentivirus (SRLV) belong to genus Lentivirus, family Retroviridae. These viruses cause caprine arthritis encephalitis (CAE) and maedi visna (MV), infectious diseases that cause economic, production, and reproductive losses. There are no effective treatments or vaccines for these diseases. Thus, early detection via serology has great importance for control of SRLV. Therefore, the objective of this review is to demonstrate the potential of the western blot (WB) test as an immunodiagnostic test for SRLV. Review: In general, immunodiagnosis of SRLV is performed via agar gel immunodiffusion (AGID) and indirect enzyme linked immunosorbent assay (ELISA), which can detect antibodies in several different biological samples but is used pref- erably with serum and blood plasma. However, WB has demonstrated efficacy in the early diagnosis of immunoglobulins against SRLV, presenting higher sensitivity and specificity than the serological tests usually used, because this technique can detect antibodies at a dilution as much as 256 times greater than that of AGID and 32 times greater than that of ELISA. SRLV infection and consequent immunological activation result in the induction of cellular and humoral responses. Ad-ditionally, around the third week, production of antibodies directed mainly toward viral capsid proteins (p25 and p28) occurs. After the fifth week, production of immunoglobulins directed toward other viral proteins occurs. Because of the persistence of SRLV infection, serology is considered to be the most practical means to diagnosis. Each serological test has a percentage specificity and distinct sensitivity, as well as advantages and disadvantages in its applicability. It should be noted that there is no gold standard test for diagnosis of SRLV infection. Moreover, SRLV are characterized by escape mechanisms such as genetic diversity, mutagenic potential, viral intermittence, and the process of compartmentalization, which make immunodiagnosis more difficult. In addition, positive animals tend to present unstable levels of antibodies over weeks, months, and even years. In this context, WB, with early antibody detection, has been proven to be a refined and more accurate technique than other immunodiagnostic tests for SRLV. WB allows the simultaneous resolution of several immunogenic antigens present in a sample, and this feature provides it with greater reliability, differentiates it from other immunological methods, and accredits it as a test of wide applicability. Epidemiological and immunological dynamics studies often use WB in the immunodynamic diagnosis of SRLV. Serum, blood plasma, and seminal plasma are typical biological materials used in the serological diagnosis of SRLV with WB, expanding its potential as an immunodiagnosis method. Conclusion: WB is the most accurate serological technique for SRLV. It is more capable of accurate diagnosis because the genetic diversity that characterizes such lentiviruses and their various immune system escape mechanisms routinely hinder traditional diagnosis. Additionally, this test has been used widely in studies of SRLV for various purposes, but mainly in studies of epidemiological and immunological dynamics, using serum, blood plasma, or seminal plasma. However, independently of the biological sample tested, WB maintains high sensitivity and precision in immunodiagnosis, making it a refined and valid technique for SRLV control programs. MenosBackground: Small ruminant lentivirus (SRLV) belong to genus Lentivirus, family Retroviridae. These viruses cause caprine arthritis encephalitis (CAE) and maedi visna (MV), infectious diseases that cause economic, production, and reproductive losses. There are no effective treatments or vaccines for these diseases. Thus, early detection via serology has great importance for control of SRLV. Therefore, the objective of this review is to demonstrate the potential of the western blot (WB) test as an immunodiagnostic test for SRLV. Review: In general, immunodiagnosis of SRLV is performed via agar gel immunodiffusion (AGID) and indirect enzyme linked immunosorbent assay (ELISA), which can detect antibodies in several different biological samples but is used pref- erably with serum and blood plasma. However, WB has demonstrated efficacy in the early diagnosis of immunoglobulins against SRLV, presenting higher sensitivity and specificity than the serological tests usually used, because this technique can detect antibodies at a dilution as much as 256 times greater than that of AGID and 32 times greater than that of ELISA. SRLV infection and consequent immunological activation result in the induction of cellular and humoral responses. Ad-ditionally, around the third week, production of antibodies directed mainly toward viral capsid proteins (p25 and p28) occurs. After the fifth week, production of immunoglobulins directed toward other viral proteins occurs. Because of the persistenc... Mostrar Tudo |
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Palavras-Chave: |
Diagnóstico sorológico; Lentivirose; Lentiviruses; LVPR; Serological diagnosis; SRLV. |
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Thesagro: |
Caprino; Doença Animal; Imunoglobulina; Ovino. |
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Thesaurus Nal: |
Caprine arthritis-encephalitis; Disease diagnosis; Goat diseases; Goats; Immunoglobulins; Serodiagnosis; Sheep diseases; Visna maedi virus; Western blotting. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228882/1/cnpc-2021-Art-91.pdf
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Marc: |
LEADER 04531naa a2200385 a 4500
001 2137467
005 2021-12-10
008 2021 bl uuuu u00u1 u #d
100 1 $aPEIXOTO, R. M.
245 $aWestern Blot no imunodiagnóstico de lentivírus de pequenos ruminantes.$h[electronic resource]
260 $c2021
520 $aBackground: Small ruminant lentivirus (SRLV) belong to genus Lentivirus, family Retroviridae. These viruses cause caprine arthritis encephalitis (CAE) and maedi visna (MV), infectious diseases that cause economic, production, and reproductive losses. There are no effective treatments or vaccines for these diseases. Thus, early detection via serology has great importance for control of SRLV. Therefore, the objective of this review is to demonstrate the potential of the western blot (WB) test as an immunodiagnostic test for SRLV. Review: In general, immunodiagnosis of SRLV is performed via agar gel immunodiffusion (AGID) and indirect enzyme linked immunosorbent assay (ELISA), which can detect antibodies in several different biological samples but is used pref- erably with serum and blood plasma. However, WB has demonstrated efficacy in the early diagnosis of immunoglobulins against SRLV, presenting higher sensitivity and specificity than the serological tests usually used, because this technique can detect antibodies at a dilution as much as 256 times greater than that of AGID and 32 times greater than that of ELISA. SRLV infection and consequent immunological activation result in the induction of cellular and humoral responses. Ad-ditionally, around the third week, production of antibodies directed mainly toward viral capsid proteins (p25 and p28) occurs. After the fifth week, production of immunoglobulins directed toward other viral proteins occurs. Because of the persistence of SRLV infection, serology is considered to be the most practical means to diagnosis. Each serological test has a percentage specificity and distinct sensitivity, as well as advantages and disadvantages in its applicability. It should be noted that there is no gold standard test for diagnosis of SRLV infection. Moreover, SRLV are characterized by escape mechanisms such as genetic diversity, mutagenic potential, viral intermittence, and the process of compartmentalization, which make immunodiagnosis more difficult. In addition, positive animals tend to present unstable levels of antibodies over weeks, months, and even years. In this context, WB, with early antibody detection, has been proven to be a refined and more accurate technique than other immunodiagnostic tests for SRLV. WB allows the simultaneous resolution of several immunogenic antigens present in a sample, and this feature provides it with greater reliability, differentiates it from other immunological methods, and accredits it as a test of wide applicability. Epidemiological and immunological dynamics studies often use WB in the immunodynamic diagnosis of SRLV. Serum, blood plasma, and seminal plasma are typical biological materials used in the serological diagnosis of SRLV with WB, expanding its potential as an immunodiagnosis method. Conclusion: WB is the most accurate serological technique for SRLV. It is more capable of accurate diagnosis because the genetic diversity that characterizes such lentiviruses and their various immune system escape mechanisms routinely hinder traditional diagnosis. Additionally, this test has been used widely in studies of SRLV for various purposes, but mainly in studies of epidemiological and immunological dynamics, using serum, blood plasma, or seminal plasma. However, independently of the biological sample tested, WB maintains high sensitivity and precision in immunodiagnosis, making it a refined and valid technique for SRLV control programs.
650 $aCaprine arthritis-encephalitis
650 $aDisease diagnosis
650 $aGoat diseases
650 $aGoats
650 $aImmunoglobulins
650 $aSerodiagnosis
650 $aSheep diseases
650 $aVisna maedi virus
650 $aWestern blotting
650 $aCaprino
650 $aDoença Animal
650 $aImunoglobulina
650 $aOvino
653 $aDiagnóstico sorológico
653 $aLentivirose
653 $aLentiviruses
653 $aLVPR
653 $aSerological diagnosis
653 $aSRLV
700 1 $aSOUSA, A. L. M. de
700 1 $aARAÚJO, J. F.
700 1 $aPINHEIRO, R. R.
773 $tActa Scientiae Veterinariae$gv. 49, pub. 1781, p. 1-11, 2021.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
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Data corrente: |
25/04/2023 |
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Data da última atualização: |
24/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
OLIVEIRA, C. F. de; SOUZA, J. E. de; SIQUEIRA, M. J. da S.; SILVA JÚNIOR, A. C. da; FERREIRA, R. de P.; VILELA, D.; CRUZ, C. D. |
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Afiliação: |
CRISTIANO FERREIRA DE OLIVEIRA, Universidade Federal de Viçosa; JACQUELINE ENEQUIO DE SOUZA, Universidade Federal de Viçosa; MICHELE JORGE DA SILVA SIQUEIRA, Escola Superior de Agricultura Luiz de Queiroz; ANTÔNIO CARLOS DA SILVA JÚNIOR, Universidade Federal de Viçosa; REINALDO DE PAULA FERREIRA, CPPSE; DUARTE VILELA, CNPGL; COSME DAMIÃO CRUZ, Universidade Federal de Viçosa. |
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Título: |
Selection of alfalfa genotypes for dry matter yield and persistence with repeated measures. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Agronomy Science and Biotechnology, v. 9, p. 1-14, 2023. |
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DOI: |
https://doi.org/10.33158/ASB.r177.v9 |
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Idioma: |
Inglês |
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Conteúdo: |
The biggest challenge in the alfalfa breeding program is to obtain cultivars with high persistence, high productivity, and adaptability. Therefore, studies about selection methods are necessary for the success of alfalfa breeding programs. This study aimed to evaluate dry matter yield and persistence in alfalfa for selecting genotypes, using appropriate statistical models for experiments with repeated measures. The experiment was conducted at Embrapa Southeast Livestock, in São Carlos, state of São Paulo, Brazil in a randomized blocks design, in plots subdivided in time, with three replicates. Eight genotypes were evaluated, and the agronomic trait evaluated was dry matter yield. The experiments in split-plots were used with two and three errors and generalized linear models with the following correlation structures: composite symmetry (CS), heterogeneous composite symmetry (HCS), auto regressive (AR), heterogeneous auto regressive (HAR), and variance components (VC). The best model was selected according to the lowest value of the Akaike Information Criterion (AIC), and three methodologies were used to identify the genotype with greater productivity and persistence: Average test for multiple comparisons, adaptability, and stability by multi-information, and similarity between genotype and ideotype. The interaction between genotypes and cuts was significant, demonstrating the existence of the different behavior of the alfalfa genotypes over the cuts. Different methodologies allowed to measure the average yield of the alfalfa genotype and the persistence over the cuts. PSB 4 genotype demonstrated promissory behavior in terms of productivity and persistence throughout the production cycle of alfalfa. MenosThe biggest challenge in the alfalfa breeding program is to obtain cultivars with high persistence, high productivity, and adaptability. Therefore, studies about selection methods are necessary for the success of alfalfa breeding programs. This study aimed to evaluate dry matter yield and persistence in alfalfa for selecting genotypes, using appropriate statistical models for experiments with repeated measures. The experiment was conducted at Embrapa Southeast Livestock, in São Carlos, state of São Paulo, Brazil in a randomized blocks design, in plots subdivided in time, with three replicates. Eight genotypes were evaluated, and the agronomic trait evaluated was dry matter yield. The experiments in split-plots were used with two and three errors and generalized linear models with the following correlation structures: composite symmetry (CS), heterogeneous composite symmetry (HCS), auto regressive (AR), heterogeneous auto regressive (HAR), and variance components (VC). The best model was selected according to the lowest value of the Akaike Information Criterion (AIC), and three methodologies were used to identify the genotype with greater productivity and persistence: Average test for multiple comparisons, adaptability, and stability by multi-information, and similarity between genotype and ideotype. The interaction between genotypes and cuts was significant, demonstrating the existence of the different behavior of the alfalfa genotypes over the cuts. Different methodologies ... Mostrar Tudo |
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Thesagro: |
Biometria; Genótipo; Matéria Seca; Medicago Sativa. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153333/1/Selection-of-alfalfa-genotypes-for-dry-matter.pdf
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Marc: |
LEADER 02500naa a2200253 a 4500
001 2153967
005 2023-05-24
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.33158/ASB.r177.v9$2DOI
100 1 $aOLIVEIRA, C. F. de
245 $aSelection of alfalfa genotypes for dry matter yield and persistence with repeated measures.$h[electronic resource]
260 $c2023
520 $aThe biggest challenge in the alfalfa breeding program is to obtain cultivars with high persistence, high productivity, and adaptability. Therefore, studies about selection methods are necessary for the success of alfalfa breeding programs. This study aimed to evaluate dry matter yield and persistence in alfalfa for selecting genotypes, using appropriate statistical models for experiments with repeated measures. The experiment was conducted at Embrapa Southeast Livestock, in São Carlos, state of São Paulo, Brazil in a randomized blocks design, in plots subdivided in time, with three replicates. Eight genotypes were evaluated, and the agronomic trait evaluated was dry matter yield. The experiments in split-plots were used with two and three errors and generalized linear models with the following correlation structures: composite symmetry (CS), heterogeneous composite symmetry (HCS), auto regressive (AR), heterogeneous auto regressive (HAR), and variance components (VC). The best model was selected according to the lowest value of the Akaike Information Criterion (AIC), and three methodologies were used to identify the genotype with greater productivity and persistence: Average test for multiple comparisons, adaptability, and stability by multi-information, and similarity between genotype and ideotype. The interaction between genotypes and cuts was significant, demonstrating the existence of the different behavior of the alfalfa genotypes over the cuts. Different methodologies allowed to measure the average yield of the alfalfa genotype and the persistence over the cuts. PSB 4 genotype demonstrated promissory behavior in terms of productivity and persistence throughout the production cycle of alfalfa.
650 $aBiometria
650 $aGenótipo
650 $aMatéria Seca
650 $aMedicago Sativa
700 1 $aSOUZA, J. E. de
700 1 $aSIQUEIRA, M. J. da S.
700 1 $aSILVA JÚNIOR, A. C. da
700 1 $aFERREIRA, R. de P.
700 1 $aVILELA, D.
700 1 $aCRUZ, C. D.
773 $tAgronomy Science and Biotechnology$gv. 9, p. 1-14, 2023.
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Embrapa Pecuária Sudeste (CPPSE) |
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