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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Semiárido. |
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Data corrente: |
10/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, A. S.; PEREIRA, L. G. R.; PEDREIRA, M. dos S.; MACHADO, F. S.; CAMPOS, M. M.; CORTINHAS C. S.; ACEDO, T. S.; SANTOS, R. D. dos; RODRIGUES, J. P. P.; MAURICIO, R. M.; TOMICH, T. R. |
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Afiliação: |
Abias Santos Silva; LUIZ GUSTAVO RIBEIRO PEREIRA, CNPGL; Márcio dos Santos Pedreira; FERNANDA SAMARINI MACHADO, CNPGL; MARIANA MAGALHAES CAMPOS, CNPGL; Cristina Simões Cortinhas; Tiago Sabella Acedo; RAFAEL DANTAS DOS SANTOS, CPATSA; João Paulo Pacheco Rodrigues; Rogério Martins Maurício; THIERRY RIBEIRO TOMICH, CNPGL. |
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Título: |
Effects of exogenous amylase on the in vitro digestion kinetics of whole-crop maize silages made from flint or dent grain type at different phenological stages grown in tropical condition. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Animal Physiololgy and Animal Nutrition, v. 104, n. 1, p. 76-87, 2020. |
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DOI: |
https://doi.org/10.1111/jpn.13234 |
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Idioma: |
Inglês |
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Conteúdo: |
The effect of exogenous amylase on the in vitro rumen digestion kinetics of wholecrop maize silage made from dent (RB9004) or flint grain type (RB9308) was evaluated at different phenological stages: soft dough (SOD), early dent (EAD), ½ milkline (½M) and ¾ milkline (¾M). Forage was harvested from 70 to 110 days after sowing. Two rumen-cannulated cows receiving or not exogenous amylase (0.7 g/kg dry matter-DM, provided to achieve 396 kilo Novo units of amylase activity/kg of TMR DM) were used as donor of ruminal fluid. The in vitro gas production kinetics was evaluated according to a dual?pool logistic model. The chemical composition and gas production kinetics were affected by the hybrid and phenological stages. The flint hybrid had lower range for chemical analysis among physiological stages. Harvesting at ½M and ¾M improved DM content, bromatological composition and silage quality parameters compared to dent or flint types. Amylase (i) increased methane (CH4) production and in vitro dry matter digestibility (IVDMD) in ½M stage, (ii) improved digestion kinetics by reducing lag time and increasing total gas production and fermentation rates of non?fibrous carbohydrates (NFC) and fibrous carbohydrates (FC), and (iii) increased extent and fermentation rate of NFC and increased fermentation rate of FC fraction in whole?crop maize silages produced from dent or flint types in all phenological stages. Harvesting between ½M and ¾M is the best phenological stage to improve chemical composition and silage quality parameters. Exogenous amylase showed improvements on fibre digestion of silages at ½M and ¾M phenological stages in both grain types of corn. MenosThe effect of exogenous amylase on the in vitro rumen digestion kinetics of wholecrop maize silage made from dent (RB9004) or flint grain type (RB9308) was evaluated at different phenological stages: soft dough (SOD), early dent (EAD), ½ milkline (½M) and ¾ milkline (¾M). Forage was harvested from 70 to 110 days after sowing. Two rumen-cannulated cows receiving or not exogenous amylase (0.7 g/kg dry matter-DM, provided to achieve 396 kilo Novo units of amylase activity/kg of TMR DM) were used as donor of ruminal fluid. The in vitro gas production kinetics was evaluated according to a dual?pool logistic model. The chemical composition and gas production kinetics were affected by the hybrid and phenological stages. The flint hybrid had lower range for chemical analysis among physiological stages. Harvesting at ½M and ¾M improved DM content, bromatological composition and silage quality parameters compared to dent or flint types. Amylase (i) increased methane (CH4) production and in vitro dry matter digestibility (IVDMD) in ½M stage, (ii) improved digestion kinetics by reducing lag time and increasing total gas production and fermentation rates of non?fibrous carbohydrates (NFC) and fibrous carbohydrates (FC), and (iii) increased extent and fermentation rate of NFC and increased fermentation rate of FC fraction in whole?crop maize silages produced from dent or flint types in all phenological stages. Harvesting between ½M and ¾M is the best phenological stage to improve chemical... Mostrar Tudo |
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Palavras-Chave: |
Carboidratos; Digestão in vitro; Tempo de colheita. |
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Thesagro: |
Digestibilidade; Enzima; Metano; Milho; Silagem. |
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Thesaurus Nal: |
Corn silage; Grass silage; Silage. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02879naa a2200385 a 4500
001 2118526
005 2024-02-06
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/jpn.13234$2DOI
100 1 $aSILVA, A. S.
245 $aEffects of exogenous amylase on the in vitro digestion kinetics of whole-crop maize silages made from flint or dent grain type at different phenological stages grown in tropical condition.$h[electronic resource]
260 $c2020
520 $aThe effect of exogenous amylase on the in vitro rumen digestion kinetics of wholecrop maize silage made from dent (RB9004) or flint grain type (RB9308) was evaluated at different phenological stages: soft dough (SOD), early dent (EAD), ½ milkline (½M) and ¾ milkline (¾M). Forage was harvested from 70 to 110 days after sowing. Two rumen-cannulated cows receiving or not exogenous amylase (0.7 g/kg dry matter-DM, provided to achieve 396 kilo Novo units of amylase activity/kg of TMR DM) were used as donor of ruminal fluid. The in vitro gas production kinetics was evaluated according to a dual?pool logistic model. The chemical composition and gas production kinetics were affected by the hybrid and phenological stages. The flint hybrid had lower range for chemical analysis among physiological stages. Harvesting at ½M and ¾M improved DM content, bromatological composition and silage quality parameters compared to dent or flint types. Amylase (i) increased methane (CH4) production and in vitro dry matter digestibility (IVDMD) in ½M stage, (ii) improved digestion kinetics by reducing lag time and increasing total gas production and fermentation rates of non?fibrous carbohydrates (NFC) and fibrous carbohydrates (FC), and (iii) increased extent and fermentation rate of NFC and increased fermentation rate of FC fraction in whole?crop maize silages produced from dent or flint types in all phenological stages. Harvesting between ½M and ¾M is the best phenological stage to improve chemical composition and silage quality parameters. Exogenous amylase showed improvements on fibre digestion of silages at ½M and ¾M phenological stages in both grain types of corn.
650 $aCorn silage
650 $aGrass silage
650 $aSilage
650 $aDigestibilidade
650 $aEnzima
650 $aMetano
650 $aMilho
650 $aSilagem
653 $aCarboidratos
653 $aDigestão in vitro
653 $aTempo de colheita
700 1 $aPEREIRA, L. G. R.
700 1 $aPEDREIRA, M. dos S.
700 1 $aMACHADO, F. S.
700 1 $aCAMPOS, M. M.
700 1 $aCORTINHAS C. S.
700 1 $aACEDO, T. S.
700 1 $aSANTOS, R. D. dos
700 1 $aRODRIGUES, J. P. P.
700 1 $aMAURICIO, R. M.
700 1 $aTOMICH, T. R.
773 $tJournal of Animal Physiololgy and Animal Nutrition$gv. 104, n. 1, p. 76-87, 2020.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Tipo/Formato |
Classificação |
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Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
17/11/2022 |
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Data da última atualização: |
14/07/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ZWAR, I. P.; TROTTA, C. do V.; ZIOTTI, A. B. S.; LIMA NETO, M.; ARAÚJO, W. L. de; MELO, I. S. de; OTTONI, C. A.; SOUZA, A. O. de. |
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Afiliação: |
INGRID PADOVESE ZWAR, INSTITUTO BUTANTAN; CATERINA DO VALLE TROTTA, UNIVERSIDADE ESTADUAL PAULISTA; ANA BEATRIZ SICCHIERI ZIOTTI, UNIVERSIDADE ESTADUAL PAULISTA; MILTON LIMA NETO, UNIVERSIDADE ESTADUAL PAULISTA; WELINGTON LUIZ DE ARAÚJO, UNIVERSIDADE DE SÃO PAULO; ITAMAR SOARES DE MELO, CNPMA; CRISTIANE ANGÉLICA OTTONI, UNIVERSIDADE ESTADUAL PAULISTA; ANA OLÍVIA DE SOUZA, INSTITUTO BUTANTAN. |
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Título: |
Biosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Journal of Basic Microbiology, v. 63, n. 1, p. 64-74, 2022. |
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ISSN: |
0233-111X |
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DOI: |
https://doi.org/10.1002/jobm.202200439 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: To find effective silver nanoparticles (AgNPs) for control of phytopathogens, in this study, two strains of actinomycetes isolated from the soil of the Brazilian biome Caatinga (Caat5-35) and from mangrove sediment (Canv1-58) were utilized. The strains were identified by using the 16S rRNA gene sequencing as Streptomyces sp., related to Streptomyces mimosus species. The obtained AgNPs were coded as AgNPs 35 and AgNPs58 and characterized by size and morphology using dynamic light scattering, zeta potential, transmission electron microscopy, and Fourier transformed infrared (FTIR). The antifungal activity of the AgNPs35 and AgNPs58 was evaluated in vitro by the minimal inhibitory concentration (MIC) assay on the phytopathogens, Alternaria solani, Alternaria alternata, and Colletotrichum gloeosporioides. The phytotoxic effect was evaluated by the germination rate and seedling growth of rice (Oryza sativa). AgNPs35 and AgNPs58 showed surface plasmon resonance and average sizes of 30 and 60 nm, respectively. Both AgNPs presented spherical shape and the FTIR analysis confirmed the presence of functional groups such as free amines and hydroxyls of biomolecules bounded to the external layer of the nanoparticles. Both AgNPs inhibited the growth of the three phytopathogens tested, and A. alternate was the most sensible (MIC < = 4 µM). Moreover, the AgNPs35 and AgNPs58 did not induce phytotoxic effects on the germination and development of rice seedlings. In conclusion, these AgNPs are promising candidates to biocontrol of these phytopathogens without endangering rice plants. MenosAbstract: To find effective silver nanoparticles (AgNPs) for control of phytopathogens, in this study, two strains of actinomycetes isolated from the soil of the Brazilian biome Caatinga (Caat5-35) and from mangrove sediment (Canv1-58) were utilized. The strains were identified by using the 16S rRNA gene sequencing as Streptomyces sp., related to Streptomyces mimosus species. The obtained AgNPs were coded as AgNPs 35 and AgNPs58 and characterized by size and morphology using dynamic light scattering, zeta potential, transmission electron microscopy, and Fourier transformed infrared (FTIR). The antifungal activity of the AgNPs35 and AgNPs58 was evaluated in vitro by the minimal inhibitory concentration (MIC) assay on the phytopathogens, Alternaria solani, Alternaria alternata, and Colletotrichum gloeosporioides. The phytotoxic effect was evaluated by the germination rate and seedling growth of rice (Oryza sativa). AgNPs35 and AgNPs58 showed surface plasmon resonance and average sizes of 30 and 60 nm, respectively. Both AgNPs presented spherical shape and the FTIR analysis confirmed the presence of functional groups such as free amines and hydroxyls of biomolecules bounded to the external layer of the nanoparticles. Both AgNPs inhibited the growth of the three phytopathogens tested, and A. alternate was the most sensible (MIC < = 4 µM). Moreover, the AgNPs35 and AgNPs58 did not induce phytotoxic effects on the germination and development of rice seedlings. In conclusion, these... Mostrar Tudo |
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Thesagro: |
Actinomiceto; Controle Biológico; Doença de Planta. |
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Thesaurus NAL: |
Actinomyces; Biological control agents; Fungal diseases of plants; Nanoparticles; Nanosilver. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02634naa a2200325 a 4500
001 2148405
005 2023-07-14
008 2022 bl uuuu u00u1 u #d
022 $a0233-111X
024 7 $ahttps://doi.org/10.1002/jobm.202200439$2DOI
100 1 $aZWAR, I. P.
245 $aBiosynthesis of silver nanoparticles using actinomycetes, phytotoxicity on rice seeds, and potential application in the biocontrol of phytopathogens.$h[electronic resource]
260 $c2022
520 $aAbstract: To find effective silver nanoparticles (AgNPs) for control of phytopathogens, in this study, two strains of actinomycetes isolated from the soil of the Brazilian biome Caatinga (Caat5-35) and from mangrove sediment (Canv1-58) were utilized. The strains were identified by using the 16S rRNA gene sequencing as Streptomyces sp., related to Streptomyces mimosus species. The obtained AgNPs were coded as AgNPs 35 and AgNPs58 and characterized by size and morphology using dynamic light scattering, zeta potential, transmission electron microscopy, and Fourier transformed infrared (FTIR). The antifungal activity of the AgNPs35 and AgNPs58 was evaluated in vitro by the minimal inhibitory concentration (MIC) assay on the phytopathogens, Alternaria solani, Alternaria alternata, and Colletotrichum gloeosporioides. The phytotoxic effect was evaluated by the germination rate and seedling growth of rice (Oryza sativa). AgNPs35 and AgNPs58 showed surface plasmon resonance and average sizes of 30 and 60 nm, respectively. Both AgNPs presented spherical shape and the FTIR analysis confirmed the presence of functional groups such as free amines and hydroxyls of biomolecules bounded to the external layer of the nanoparticles. Both AgNPs inhibited the growth of the three phytopathogens tested, and A. alternate was the most sensible (MIC < = 4 µM). Moreover, the AgNPs35 and AgNPs58 did not induce phytotoxic effects on the germination and development of rice seedlings. In conclusion, these AgNPs are promising candidates to biocontrol of these phytopathogens without endangering rice plants.
650 $aActinomyces
650 $aBiological control agents
650 $aFungal diseases of plants
650 $aNanoparticles
650 $aNanosilver
650 $aActinomiceto
650 $aControle Biológico
650 $aDoença de Planta
700 1 $aTROTTA, C. do V.
700 1 $aZIOTTI, A. B. S.
700 1 $aLIMA NETO, M.
700 1 $aARAÚJO, W. L. de
700 1 $aMELO, I. S. de
700 1 $aOTTONI, C. A.
700 1 $aSOUZA, A. O. de
773 $tJournal of Basic Microbiology$gv. 63, n. 1, p. 64-74, 2022.
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Embrapa Meio Ambiente (CNPMA) |
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