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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Instrumentação. |
Data corrente: |
20/04/2023 |
Data da última atualização: |
18/07/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PEREA, G. N. R.; PAVINATTO, A.; SCHNEIDER, R.; MUNK, M.; BRANDAO, H. de M.; CORREA, D. S. |
Afiliação: |
Universidade de São Paulo; Universidade Federal de São Carlos; Universidade Federal de Juiz de Fora; HUMBERTO DE MELLO BRANDAO, CNPGL; DANIEL SOUZA CORREA, CNPDIA. |
Título: |
Electrospun nanofibers based on polyvinylpyrrolidone/chitosan and cloxacillin: investigation of morphological features, antibiotic release and antimicrobial properties. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Journal of Polymer Research, v. 30, 166, 2023. |
DOI: |
https://doi.org/10.1007/s10965-023-03535-7 |
Idioma: |
Inglês |
Conteúdo: |
Polymer nanofbers possess a range of attractive features that make them highly suitable for various biomedical application, including their high surface area/volume ratio, mechanical fexibility, porosity and potential for surface or bulk functionalization. Besides, such nanofbers can be used to encapsulate antibiotics to fabricate slow-release systems, but in this case the nanofbers should be fabricated with biocompatible and biodegradable polymers. Here we developed a novel nanocarrier system based on polyvinylpyrrolidone (PVP)/chitosan (CHI) electrospun nanofbers containing cloxacillin benzathine (CLO). PVP was chosen because of its ability to form nanofbers, while a small amount of chitosan was added to help control the CLO release, which antibiotic is widely employed to treat bacterial infections. The as-produced CLO-containing electrospun nanofbers were characterized regarding their morphological features and antibiotic release profle. SEM images revealed homogeneous and defectsfree nanofbers, exhibiting no discernible morphological variations between the CLO-containing and CLO-non-containing nanofbers. FTIR results indicate hydrogen bonds formation between carboxyl and carboxylate groups from CLO and hydroxyl groups from CHI. The antimicrobial properties of antibiotic-loaded nanofbers were investigated against Gram-positive bacteria S. aureus, yielding inhibition zone diameters of 12.5±1.6 mm and 29.8±1.3 mm for PVP/CHI/CLO and PVP/CLO nanofbers, respectively. Besides, the drug release kinetics study indicates that the nanocarrier system formed by PVP/CHI can release the antibiotics more slowly due to the chemical interactions between CHI and CLO compounds. Our results suggest that drug activity was preserved after encapsulation by electrospinning, and the diference in the inhibition zone diameter was corroborated by drug release mechanism experiments, indicating the system as a potential material to treat infections caused by S. aureus MenosPolymer nanofbers possess a range of attractive features that make them highly suitable for various biomedical application, including their high surface area/volume ratio, mechanical fexibility, porosity and potential for surface or bulk functionalization. Besides, such nanofbers can be used to encapsulate antibiotics to fabricate slow-release systems, but in this case the nanofbers should be fabricated with biocompatible and biodegradable polymers. Here we developed a novel nanocarrier system based on polyvinylpyrrolidone (PVP)/chitosan (CHI) electrospun nanofbers containing cloxacillin benzathine (CLO). PVP was chosen because of its ability to form nanofbers, while a small amount of chitosan was added to help control the CLO release, which antibiotic is widely employed to treat bacterial infections. The as-produced CLO-containing electrospun nanofbers were characterized regarding their morphological features and antibiotic release profle. SEM images revealed homogeneous and defectsfree nanofbers, exhibiting no discernible morphological variations between the CLO-containing and CLO-non-containing nanofbers. FTIR results indicate hydrogen bonds formation between carboxyl and carboxylate groups from CLO and hydroxyl groups from CHI. The antimicrobial properties of antibiotic-loaded nanofbers were investigated against Gram-positive bacteria S. aureus, yielding inhibition zone diameters of 12.5±1.6 mm and 29.8±1.3 mm for PVP/CHI/CLO and PVP/CLO nanofbers, respectively. Besides,... Mostrar Tudo |
Palavras-Chave: |
Antibiotic release; Electrospinning; Eletrofiação; Mastite; Slow-release. |
Thesagro: |
Antibiótico; Bovino; Doença Animal; Tratamento. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02915naa a2200301 a 4500 001 2153293 005 2023-07-18 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s10965-023-03535-7$2DOI 100 1 $aPEREA, G. N. R. 245 $aElectrospun nanofibers based on polyvinylpyrrolidone/chitosan and cloxacillin$binvestigation of morphological features, antibiotic release and antimicrobial properties.$h[electronic resource] 260 $c2023 520 $aPolymer nanofbers possess a range of attractive features that make them highly suitable for various biomedical application, including their high surface area/volume ratio, mechanical fexibility, porosity and potential for surface or bulk functionalization. Besides, such nanofbers can be used to encapsulate antibiotics to fabricate slow-release systems, but in this case the nanofbers should be fabricated with biocompatible and biodegradable polymers. Here we developed a novel nanocarrier system based on polyvinylpyrrolidone (PVP)/chitosan (CHI) electrospun nanofbers containing cloxacillin benzathine (CLO). PVP was chosen because of its ability to form nanofbers, while a small amount of chitosan was added to help control the CLO release, which antibiotic is widely employed to treat bacterial infections. The as-produced CLO-containing electrospun nanofbers were characterized regarding their morphological features and antibiotic release profle. SEM images revealed homogeneous and defectsfree nanofbers, exhibiting no discernible morphological variations between the CLO-containing and CLO-non-containing nanofbers. FTIR results indicate hydrogen bonds formation between carboxyl and carboxylate groups from CLO and hydroxyl groups from CHI. The antimicrobial properties of antibiotic-loaded nanofbers were investigated against Gram-positive bacteria S. aureus, yielding inhibition zone diameters of 12.5±1.6 mm and 29.8±1.3 mm for PVP/CHI/CLO and PVP/CLO nanofbers, respectively. Besides, the drug release kinetics study indicates that the nanocarrier system formed by PVP/CHI can release the antibiotics more slowly due to the chemical interactions between CHI and CLO compounds. Our results suggest that drug activity was preserved after encapsulation by electrospinning, and the diference in the inhibition zone diameter was corroborated by drug release mechanism experiments, indicating the system as a potential material to treat infections caused by S. aureus 650 $aAntibiótico 650 $aBovino 650 $aDoença Animal 650 $aTratamento 653 $aAntibiotic release 653 $aElectrospinning 653 $aEletrofiação 653 $aMastite 653 $aSlow-release 700 1 $aPAVINATTO, A. 700 1 $aSCHNEIDER, R. 700 1 $aMUNK, M. 700 1 $aBRANDAO, H. de M. 700 1 $aCORREA, D. S. 773 $tJournal of Polymer Research$gv. 30, 166, 2023.
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Embrapa Instrumentação (CNPDIA) |
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Registros recuperados : 27 | |
1. | | PAVINATTO, A.; MERCANTE, L. A.; CORREA, D. S. Filmes nanoestruturados contendo quitosana para aplicação como plataforma de biossensor eletroquímico. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 2014, São Carlos, SP Anais do SIAGRO: ciência, inovação e mercado 2014. São Carlos, SP: Embrapa Instrumentação, 2014. p. 411-414. Editores: Carlos Manoel Pedro Vaz, Débora Marcondes Bastos Pereira Milori, Silvio Crestana.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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2. | | PAVINATTO, A.; MERCANTE, L. A.; CORREA, D. S. Filmes nanoestruturados contendo quitosana para aplicação como plataforma sensorial. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 8, 2014, Juiz de fora. Anais... São Carlos: Embrapa Instrumentação; Campo Grande: Embrapa Gado de Corte; Juiz de Fora: Embrapa Gado de Leite, 2014. p. 40-42. Editores: Maria Alice Martins, Humberto de Mello Brandão, Marlene de Barros Coelho, Daniel Souza Corrêa, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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6. | | MIGLIORINI, F. L.; SANFELICE, R. C.; PAVINATTO, A.; STEFFENS, J.; STEFFENS, C.; CORREA, D. S. Electrospun nanofibers based on polyamide 6/chitosan modified with metal nanoparticles for application in a chemical sensor. In: BRAZIL MRS MEETING - SBPMAT, 15, 2016, Rio de Janeiro. Proceedings... Rio de Janeiro: SBPMat, 2016. não paginado.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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7. | | MIGLIORINI, F. L.; SANFELICE, R. C.; PAVINATTO, A.; STEFFENS, J.; STEFFENS, C.; CORREA, D. S. Electrospun nanofibers based on polyamide 6/chitosan modified with metal nanoparticles for application in a chemical sensor. In: BRAZIL MRS MEETING - SBPMAT, 15, 2016, Rio de Janeiro. Proceedings... Rio de Janeiro: SBPMat, 2016. p. 919.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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8. | | PAVINATTO, A.; MERCANTE, L. A.; FACURE, L. H. C.; MATTOSO, L. H. C.; CORREA, D. S. Nanofibras eletrofiadas de PVP/quitosana/óxido de grafeno reduzido para aplicação em biossensores. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 9., 2017, São Carlos. Anais ... São Carlos: Embrapa Instrumentação, 2017. p. 71-73. Editores: Caue Ribeiro de Oliveira, Elaine Cristina Paris, Luiz Henrique Capparelli Mattoso, Marcelo Porto Bemquerer, Maria Alice Martins, Odílio Benedito Garrido de Assis.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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17. | | PEREA, G. N. R.; PAVINATTO, A.; SCHNEIDER, R.; MUNK, M.; BRANDAO, H. de M.; CORREA, D. S. Electrospun nanofibers based on polyvinylpyrrolidone/chitosan and cloxacillin: investigation of morphological features, antibiotic release and antimicrobial properties. Journal of Polymer Research, v. 30, 166, 2023.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
Biblioteca(s): Embrapa Gado de Leite; Embrapa Instrumentação. |
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19. | | ANDRE, R. S.; PAVINATTO, A.; MERCANTE, L. A.; PARIS, E. C.; MATTOSO, L. H. C.; CORREA, D. S. Improving the electrochemical properties of polyamide 6/polyaniline electrospun nanofibers by surface modification with ZnO nanoparticles. RSC advances, [Inglaterra], v. 5, n. 90, p. 73875-73881, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Instrumentação. |
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20. | | ANDRE, R. S.; PAVINATTO, A.; MERCANTE, L. A.; PARIS, E. C.; MATTOSO, L. H. C.; CORREA, D. S. Development of hybrid nanostructured platforms for chemical sensor applications. In: BRAZILIAN MRS MEETING - SBPMAT, 14, 2015, Rio de Janeiro. Program book... Rio de Janeiro: SBPMat, 2015. não paginado.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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Registros recuperados : 27 | |
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