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| 1. |  | OLIVEIRA FILHO, E. C. de; GERALDINO, B. R.; GRISOLIA, C. K.; PAUMGARTTEN, F. J. R. Acute Toxicity of Endosulfan, Nonylphenol Ethoxylate, and Ethanol to Different Life Stages of the Freshwater Snail Biomphalaria tenagophila (Orbigny, 1835). Bulletin of Environmental Contamination and Toxicology, v. 75, n. 6, p. 1185-1190, December 2005.| Biblioteca(s): Embrapa Cerrados. |
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| 15. | | OLIVEIRA-FILHO, E. C.; DA-MATTA, A. C.; CABRAL, L. L.; VEIGA, L. F.; PAUMGARTTEN, F. J. R. Comparison between four and seven-day Ceriodaphnia dubia survival and reproduction test protocols using oil refinery effluent samples. Brazilian Archives of Biology and Technology, Curitiba, v. 51, n. 1, p. 137-142, Jan./Fev. 2008.| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 15 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/09/2016 |
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Data da última atualização: |
14/02/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
REDOAN, A. C. M.; SOUSA-SILVA, C. R.; CRUZ, I. |
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Afiliação: |
ANA CAROLINA MACIEL REDOAN, UFSCAR, São Carlos, SP.; CARLOS ROBERTO SOUSA-SILVA, UFSCAR, São Carlos, SP.; IVAN CRUZ, CNPMS. |
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Título: |
Serologies use in the identification of insects predators prey. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 15, n. 2, p. 157-170, 2016. |
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DOI: |
10.18512/1980-6477/rbms.v15n2p157-170 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to produce antiserum for the main pests found in maize, Spodoptera frugiperda, Helicoverpa zea and armigera, Rhopalosiphum maidis, and uses it to determine their predators. Pest samples were macerated in 0.85% saline solution. The macerated were centrifuged and the supernatants were used as immunizing antigens to obtain the antiserum. For this purpose, a rabbit was immunized with 3.0 ml of the immunizing antigen on the lymph node region. Homologous serological tests were performed by double diffusion in agar. The homologous serological reactions were positive after seven days of antigen inoculation. The technique has sensitivity to detect predation of the pests studied. The tests were positive for a prey in the digestive tract of the predator to 96 hours of ingestion. Regarding the number of prey ingested there was no difference in the lines. It is concluded that after a single injection of antigen in rabbit lymph node region, it was possible to get an antiserum specific for pests of corn. Field tests showed that there was a certain food preference of Doru luteipes by S. frugiperda, Chrysoperla externa by aphid. |
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Palavras-Chave: |
Predação. |
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Thesagro: |
Antígeno; Antissoro; Zea mays. |
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Thesaurus NAL: |
Antigens; Antiserum; Predation. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148040/1/Serologies-use.pdf
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Marc: |
LEADER 01839naa a2200241 a 4500
001 2053654
005 2017-02-14
008 2016 bl uuuu u00u1 u #d
024 7 $a10.18512/1980-6477/rbms.v15n2p157-170$2DOI
100 1 $aREDOAN, A. C. M.
245 $aSerologies use in the identification of insects predators prey.$h[electronic resource]
260 $c2016
520 $aThis study aimed to produce antiserum for the main pests found in maize, Spodoptera frugiperda, Helicoverpa zea and armigera, Rhopalosiphum maidis, and uses it to determine their predators. Pest samples were macerated in 0.85% saline solution. The macerated were centrifuged and the supernatants were used as immunizing antigens to obtain the antiserum. For this purpose, a rabbit was immunized with 3.0 ml of the immunizing antigen on the lymph node region. Homologous serological tests were performed by double diffusion in agar. The homologous serological reactions were positive after seven days of antigen inoculation. The technique has sensitivity to detect predation of the pests studied. The tests were positive for a prey in the digestive tract of the predator to 96 hours of ingestion. Regarding the number of prey ingested there was no difference in the lines. It is concluded that after a single injection of antigen in rabbit lymph node region, it was possible to get an antiserum specific for pests of corn. Field tests showed that there was a certain food preference of Doru luteipes by S. frugiperda, Chrysoperla externa by aphid.
650 $aAntigens
650 $aAntiserum
650 $aPredation
650 $aAntígeno
650 $aAntissoro
650 $aZea mays
653 $aPredação
700 1 $aSOUSA-SILVA, C. R.
700 1 $aCRUZ, I.
773 $tRevista Brasileira de Milho e Sorgo, Sete Lagoas$gv. 15, n. 2, p. 157-170, 2016.
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Embrapa Milho e Sorgo (CNPMS) |
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