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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
09/07/2009 |
Data da última atualização: |
14/06/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
KUBO, K. S.; STUART, R. M.; FREITAS-ASTÚA, J.; ANTONIOLI-LUIZON, R.; LOCALI-FABRIS, E. C.; COLETTA-FILHO, H. D.; MACHADO, M. A.; KITAJIMA, E. W. |
Afiliação: |
Karen Sumire Kubo, APTA; R. M. Stuart, APTA; Juliana Freitas-Ástua, CNPMF; Renata Antonioli-Luizon, APTA; Eliane Cristina Locali-Fabris, APTA; Helvecio Della Coletta-Filho, APTA; Marcos Antonio Machado, APTA; Elliot Watanabe Kitajima, ESALQ. |
Título: |
Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Archives of Virology, v. 154, n. 6, p. 1009-1014, mai. 2009. |
ISSN: |
0304-8608 |
DOI: |
DOI 10.1007/s00705-009-0395-8 |
Idioma: |
Inglês |
Conteúdo: |
The variability of a fragment of the nucleocapsid gene of orchid fleck virus (OFV) was investigated by single-strand conformational polymorphism (SSCP) analysis and nucleotide sequencing. Forty-eight samples of 18 genera of orchids were collected from Brazil, Costa Rica and Australia. The SSCP analysis yielded six different band patterns, and phylogenetic analysis based on the nucleotide fragment sequence obtained in this work and six available in GenBank showed two different groups, one with isolates 023Germany and So-Japan, and other with the rest of the isolates. None of the analyses showed geographic correlation among the Brazilian strains. The data obtained in this study showed a low genetic variation in this region of the genome; the dN/dS ratio of 0.251-0.405 demonstrated a negative selective pressure that maintains the stability of the analyzed fragments. |
Palavras-Chave: |
Nuclear type. |
Thesaurus NAL: |
Orchid fleck virus. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 01736naa a2200253 a 4500 001 1654417 005 2023-06-14 008 2009 bl uuuu u00u1 u #d 022 $a0304-8608 024 7 $aDOI 10.1007/s00705-009-0395-8$2DOI 100 1 $aKUBO, K. S. 245 $aEvaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid.$h[electronic resource] 260 $c2009 520 $aThe variability of a fragment of the nucleocapsid gene of orchid fleck virus (OFV) was investigated by single-strand conformational polymorphism (SSCP) analysis and nucleotide sequencing. Forty-eight samples of 18 genera of orchids were collected from Brazil, Costa Rica and Australia. The SSCP analysis yielded six different band patterns, and phylogenetic analysis based on the nucleotide fragment sequence obtained in this work and six available in GenBank showed two different groups, one with isolates 023Germany and So-Japan, and other with the rest of the isolates. None of the analyses showed geographic correlation among the Brazilian strains. The data obtained in this study showed a low genetic variation in this region of the genome; the dN/dS ratio of 0.251-0.405 demonstrated a negative selective pressure that maintains the stability of the analyzed fragments. 650 $aOrchid fleck virus 653 $aNuclear type 700 1 $aSTUART, R. M. 700 1 $aFREITAS-ASTÚA, J. 700 1 $aANTONIOLI-LUIZON, R. 700 1 $aLOCALI-FABRIS, E. C. 700 1 $aCOLETTA-FILHO, H. D. 700 1 $aMACHADO, M. A. 700 1 $aKITAJIMA, E. W. 773 $tArchives of Virology$gv. 154, n. 6, p. 1009-1014, mai. 2009.
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