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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
08/08/2023 |
Data da última atualização: |
27/10/2023 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
PEREIRA, W. J.; PAPPAS, M. de C. R.; PAPPAS JÚNIOR, G. J. |
Afiliação: |
WENDELL JACINTO PEREIRA, University of Brasilia; MARILIA DE CASTRO RODRIGUES PAPPAS, Cenargen; GEORGIOS JOANNIS PAPPAS JÚNIOR, University of Brasilia. |
Título: |
Computational protocol for DNA methylation profiling in plants using restriction enzyme-based genome reduction. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: SHAVRUKOV, Y. (Ed.). Plant genotyping: methods and protocols. New York: Humana Press, 2023. p. 23-36. |
Série: |
(Methods in Molecular Biology, 2638) |
DOI: |
https://doi.org/10.1007/978-1-0716-3024-2_3 |
Idioma: |
Inglês |
Palavras-Chave: |
Computational protocol; Differential expression; Methylation sensitivity; Next-generation sequencing; Restriction enzymes. |
Thesaurus Nal: |
DNA methylation. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00869naa a2200229 a 4500 001 2155758 005 2023-10-27 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/978-1-0716-3024-2_3$2DOI 100 1 $aPEREIRA, W. J. 245 $aComputational protocol for DNA methylation profiling in plants using restriction enzyme-based genome reduction.$h[electronic resource] 260 $c2023 490 $a(Methods in Molecular Biology, 2638) 650 $aDNA methylation 653 $aComputational protocol 653 $aDifferential expression 653 $aMethylation sensitivity 653 $aNext-generation sequencing 653 $aRestriction enzymes 700 1 $aPAPPAS, M. de C. R. 700 1 $aPAPPAS JÚNIOR, G. J. 773 $tIn: SHAVRUKOV, Y. (Ed.). Plant genotyping: methods and protocols. New York: Humana Press, 2023. p. 23-36.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
20/12/2018 |
Data da última atualização: |
27/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
BARÔNIO, C. A.; BERNARDI, D.; NUNES, M. Z.; PASINATO, J.; GARCIA, F. R. M.; BOTTON, M. |
Afiliação: |
Cléber Antônio Barônio, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, Brasil; Daniel Bernardi, Dept of Entomology, Embrapa Clima Temperado, Pelotas, RS, Brasil; Marcelo Z. Nunes, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, BrasiL; J. Pasinato, Embrapa Uva e Vinho, Bento Gonçalves, Rio Grande do Sul, Brasil; F. R. M. Garcia, Depto de Fitossanidade, Faculdade de Agronomia, Univ Federal de Pelotas, Pelotas, Rio Grande do Sul, Brasil; MARCOS BOTTON, CNPUV. |
Título: |
Bioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera: Tephritidae). |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Neotropical Entomology, [online], dec. 2018. |
DOI: |
10.1007/s13744-018-0653-0 |
Idioma: |
Inglês |
Conteúdo: |
The development of appropriate methodologies for bioassays is of paramount importance to study the methodological factors that may interfere in the experimental design. Thus, laboratory bioassays have become increasingly important for the determination of data that are realistic and replicable. The objective of the study was to develop a bioassay methodology with the ready-to-use toxic bait Success ? 0.02CB, 96 mg L−1 of spinosad, in adults of Ceratitis capitata (Wiedemann) as a study model. The insect origin laboratory population, LT 50= 11.16 h), time of toxic bait supply (4 h, LT 50= 16.85 h), food deprivation (12 h, LT 50= 15.55 h), and the adult age (5 days, LT 50= 35.31 h) of C. capitata showed higher susceptibility and a shorter LT 50 lethal time to the Success? 0.02CB toxic bait. However, the absence or presence of a food source (LT 50≈31.17 h) during the bioassay did not affect the susceptibility of C. capitata. Further, adults of C. capitata who were deprived and in the absence of a food source (artificial diet) had higher consumption of toxic baits (mg) during treatment exposure. The use of a 12-h deprivation period, toxic baits offered for 4 h, and absence of an artificial diet for adults of C. capitata at 5 days of age during the bioassay are considered adequate for evaluating formulations of toxic baits under laboratory conditions. Keywords: Toxicity, methodology, spinosad, Mediterranean fruit fly |
Palavras-Chave: |
Mediterranean fruit fly. |
Thesaurus NAL: |
Methodology; Spinosad; Toxicity. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189131/1/Baronio2018-Article-BioassayMethodForToxicityStudi.pdf
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Marc: |
LEADER 02160naa a2200241 a 4500 001 2102300 005 2019-04-27 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1007/s13744-018-0653-0$2DOI 100 1 $aBARÔNIO, C. A. 245 $aBioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera$bTephritidae).$h[electronic resource] 260 $c2018 520 $aThe development of appropriate methodologies for bioassays is of paramount importance to study the methodological factors that may interfere in the experimental design. Thus, laboratory bioassays have become increasingly important for the determination of data that are realistic and replicable. The objective of the study was to develop a bioassay methodology with the ready-to-use toxic bait Success ? 0.02CB, 96 mg L−1 of spinosad, in adults of Ceratitis capitata (Wiedemann) as a study model. The insect origin laboratory population, LT 50= 11.16 h), time of toxic bait supply (4 h, LT 50= 16.85 h), food deprivation (12 h, LT 50= 15.55 h), and the adult age (5 days, LT 50= 35.31 h) of C. capitata showed higher susceptibility and a shorter LT 50 lethal time to the Success? 0.02CB toxic bait. However, the absence or presence of a food source (LT 50≈31.17 h) during the bioassay did not affect the susceptibility of C. capitata. Further, adults of C. capitata who were deprived and in the absence of a food source (artificial diet) had higher consumption of toxic baits (mg) during treatment exposure. The use of a 12-h deprivation period, toxic baits offered for 4 h, and absence of an artificial diet for adults of C. capitata at 5 days of age during the bioassay are considered adequate for evaluating formulations of toxic baits under laboratory conditions. Keywords: Toxicity, methodology, spinosad, Mediterranean fruit fly 650 $aMethodology 650 $aSpinosad 650 $aToxicity 653 $aMediterranean fruit fly 700 1 $aBERNARDI, D. 700 1 $aNUNES, M. Z. 700 1 $aPASINATO, J. 700 1 $aGARCIA, F. R. M. 700 1 $aBOTTON, M. 773 $tNeotropical Entomology, [online], dec. 2018.
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