Data/hora: 13/06/2024 12:26:25

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 25/01/2017
Data da última atualização: | 25/01/2017
Autoria: | SARKAR, P.; SINGH, S. P.; PANDEY, A. K.; KATHURIA, B. K.; KUMAR, S.
Título: | Application of fluorescent antibody test in the diagnosis of sheep-pox, and study of sheep-pox virus multiplication in cell-culture.
Ano de publicação: | 1980
Fonte/Imprenta: | The Indian Journal of Animal Sciences, v. 50, n. 5, p. 428-433, May. 1980.
Idioma: | Inglês
Conteúdo: | Abstract : Direct fluorescent antibody test (FAT) was used to detect the sheep pox virus (SPV) in the oedema fluid of infected sheep, with or without traces of tissue fibres. Bright apple-green fluorescence against a dark-grey background was observed under the fluorescence microscope. The virus multiplied in monolayer cultures of lamb kidney cells. Depending upon the aggregation of virus particles, the size and brightness of the fluoresced masses became gradually prominent, and covered considerable areas of the cell cytoplasm. By indirect FAT, antibodies against SPV were detected with precision by adopting a two-stage staining procedure. This method can be used to detect the antibody status of a flock.
Palavras-Chave: | Vírus da varíola ovina.
Thesagro: | Doença animal; Ovino.
Thesaurus Nal: | Animal diseases; Animal production; Capripoxvirus.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (32562-1) - ADD - AP - PP -  -  -  - DIS