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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
27/12/2021 |
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Data da última atualização: |
27/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAIXÃO, F. R. S. da; HUARTE BONNET, C.; RIBEIRO-SILVA, C. de S.; MASCARIN, G. M.; FERNANDES, E. K. K.; PEDRINI, N. |
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Afiliação: |
FLÁVIA REGINA SANTOS DA PAIXÃO, Universidad Nacional de La Plata; CARLA HUARTE BONNET, Universidad Nacional de La Plata; CÁRITA SOUZA RIBEIRO-SILVA, UFG; GABRIEL MOURA MASCARIN, CNPMA; ÉVERTON KORT KAMP FERNANDES, UFG. |
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Título: |
Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Frontiers in Fungal Biology, v. 2, Article 654737, 2021. |
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DOI: |
https://doi.org/10.3389/ffunb.2021.654737 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Metarhizium species fungi are able to produce resistant structures termed microsclerotia, formed by compact and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia; thus, they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both ultraviolet B (UV-B) radiation and heat of microsclerotia of Metarhizium robertsii strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the 16 genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes MrcatA, MrcatP, and Mrgpx; the peroxisome biogenesis factors Mrpex5 and Mrpex14/17; and the photoprotection genes Mrlac1 and Mrlac2; and Mrlac3. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in M. robertsii and confirmed that because of its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence. MenosAbstract: Metarhizium species fungi are able to produce resistant structures termed microsclerotia, formed by compact and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia; thus, they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both ultraviolet B (UV-B) radiation and heat of microsclerotia of Metarhizium robertsii strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the 16 genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes MrcatA, MrcatP, and Mrgpx; the peroxisome biogenesis factors Mrpex5 and Mrpex14/17; and the photoprotection genes Mrlac1 and Mrlac2; and Mrlac3. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in M. robertsii and ... Mostrar Tudo |
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Palavras-Chave: |
Thermotolerance; Tolerância; UV-B radiation. |
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Thesagro: |
Controle Biológico; Fungo Entomógeno; Fungo Para Controle Biológico; Radiação Solar; Raio Ultravioleta. |
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Thesaurus Nal: |
Biological control; Entomopathogenic fungi; Gene expression; Heat tolerance; Metarhizium robertsii; Oxidative stress; Ultraviolet radiation. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/229681/1/Mascarin-Tolerance-abiotic-2021.pdf
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Marc: |
LEADER 03040naa a2200373 a 4500
001 2138387
005 2021-12-27
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/ffunb.2021.654737$2DOI
100 1 $aPAIXÃO, F. R. S. da
245 $aTolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation.$h[electronic resource]
260 $c2021
520 $aAbstract: Metarhizium species fungi are able to produce resistant structures termed microsclerotia, formed by compact and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia; thus, they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both ultraviolet B (UV-B) radiation and heat of microsclerotia of Metarhizium robertsii strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the 16 genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes MrcatA, MrcatP, and Mrgpx; the peroxisome biogenesis factors Mrpex5 and Mrpex14/17; and the photoprotection genes Mrlac1 and Mrlac2; and Mrlac3. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in M. robertsii and confirmed that because of its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence.
650 $aBiological control
650 $aEntomopathogenic fungi
650 $aGene expression
650 $aHeat tolerance
650 $aMetarhizium robertsii
650 $aOxidative stress
650 $aUltraviolet radiation
650 $aControle Biológico
650 $aFungo Entomógeno
650 $aFungo Para Controle Biológico
650 $aRadiação Solar
650 $aRaio Ultravioleta
653 $aThermotolerance
653 $aTolerância
653 $aUV-B radiation
700 1 $aHUARTE BONNET, C.
700 1 $aRIBEIRO-SILVA, C. de S.
700 1 $aMASCARIN, G. M.
700 1 $aFERNANDES, E. K. K.
700 1 $aPEDRINI, N.
773 $tFrontiers in Fungal Biology$gv. 2, Article 654737, 2021.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 6 | |
| 1. |  | HUARTE-BONNET, C.; PAIXÃO, F. R. S. da; MASCARIN, G. M.; SANTANA, M.; FERNANDES, E. K. K.; PEDRINI, N. The entomopathogenic fungus Beauveria bassiana produces microsclerotia-like pellets mediated by oxidative stress and peroxisome biogenesis. Environmental Microbiology Reports, v. 11, n. 4, p. 518?524, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 2. | | PAIXÃO, F. R. S. da; MUNIZ, E. R.; CATÃO, A. M. L.; SANTOS, T. R.; LUZ, C.; MARRETO, R. N.; MASCARIN, G. M.; FERNANDES, É. K. K. Microsclerotial pellets of Metarhizium spp.: thermotolerance and bioefficacy against the cattle tick. Applied Microbiology and Biotechnology, v. 107, n. 7-8, p. 2263-2275, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 3. | | PAIXÃO, F. R. S. da; HUARTE BONNET, C.; RIBEIRO-SILVA, C. de S.; MASCARIN, G. M.; FERNANDES, E. K. K.; PEDRINI, N. Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation. Frontiers in Fungal Biology, v. 2, Article 654737, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 4. | | PAIXÃO, F. R. S.; MUNIZ, E. R.; BARRETO, L. P.; BERNARDO, C. C.; MASCARIN, G. M.; LUZ, C.; FERNANDES, E. K. K. Increased heat tolerance afforded by oil-based conidial formulations of Metarhizium anisopliae and Metarhizium robertsii. Biocontrol Science and Technology, v. 27, n. 3, p. 324-337, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 5. | | RODRIGUES, J.; CATÃO, A. M. L.; SANTOS, A. S. dos; PAIXÃO, F. R. S.; SANTOS, T. R.; MARTINEZ, J. M.; MARRETO, R. N.; MASCARIN, G. M.; FERNANDES, E. K. K.; HUMBER, R. A.; LUZ, C. Relative humidity impacts development and activity against Aedes aegypti adults by granular formulations of Metarhizium humberi microsclerotia. Applied Microbiology And Biotechnology, v. 105, n. 7, p. 2725-2736, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 6. | | SANTOS, T. R.; PAIXÃO, F. R. S. da; CATÃO, A. M. L.; MUNIZ, E. R.; RIBEIRO-SILVA, C. S.; TAVEIRA, S. F.; LUZ, C.; MASCARIN, G. M.; FERNANDES, E. K. K.; MARRETO, R. N. Inorganic pellets containing microsclerotia of Metarhizium anisopliae: a new technological platform for the biological control of the cattle tick Rhipicephalus microplus. Applied Microbiology and Biotechnology, V. 105, N. 12, p. 5001-5012, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 6 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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