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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/01/1998 |
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Data da última atualização: |
12/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TORRES, G. A.; PARENTONI, S. N.; LOPES, M. A.; PAIVA, E. |
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Afiliação: |
EMBRAPA/CNPMS; SIDNEY NETTO PARENTONI, CNPMS. |
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Título: |
A search for RFLP markers to identify genes for aluminum tolerance in maize. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Revista Brasileira de Genética, Ribeirão Preto, v. 20, n. 3, p. 459-465, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested for their ability to discriminate the parental lines. Fifty-four of these probes were polymorphic, with 46 showing codominance. These probes were hybridized with DNA from the two contrasting bulks. Three RFLPs on chromosome 8 distinguished the bulks on the basis of band intensity. DNA of individuals from the bulks was hybridized with these probes and showed the presence of heterozygous individuals in each bulk. These results suggest that in maize there is a region related to aliminum tolerance on chromosome 8. MenosThe objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested f... Mostrar Tudo |
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Palavras-Chave: |
Maize; RFLP; Tolerance; Tolerancia. |
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Thesagro: |
Alumínio; Milho; Zea Mays. |
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Thesaurus Nal: |
aluminum. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/43456/1/Search-RFLP.pdf
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Marc: |
LEADER 02698naa a2200253 a 4500
001 1478779
005 2018-06-12
008 1997 bl uuuu u00u1 u #d
100 1 $aTORRES, G. A.
245 $aA search for RFLP markers to identify genes for aluminum tolerance in maize.$h[electronic resource]
260 $c1997
520 $aThe objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested for their ability to discriminate the parental lines. Fifty-four of these probes were polymorphic, with 46 showing codominance. These probes were hybridized with DNA from the two contrasting bulks. Three RFLPs on chromosome 8 distinguished the bulks on the basis of band intensity. DNA of individuals from the bulks was hybridized with these probes and showed the presence of heterozygous individuals in each bulk. These results suggest that in maize there is a region related to aliminum tolerance on chromosome 8.
650 $aaluminum
650 $aAlumínio
650 $aMilho
650 $aZea Mays
653 $aMaize
653 $aRFLP
653 $aTolerance
653 $aTolerancia
700 1 $aPARENTONI, S. N.
700 1 $aLOPES, M. A.
700 1 $aPAIVA, E.
773 $tRevista Brasileira de Genética, Ribeirão Preto$gv. 20, n. 3, p. 459-465, 1997.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
12/05/2009 |
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Data da última atualização: |
22/08/2022 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
OSTER, A. H.; MORAES, M. G. de; VALDEBENITO-SANHUEZA, R. M.; CORRENT, A.; BENDER, R. J. |
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Afiliação: |
ANDREIA HANSEN OSTER, CNPUV; MARCELO GRAVINA DE MORAES; ROSA MARIA VALDEBENITO SANHUEZA, CNPUV; ADRIANA CORRENT; RENAR JOÃO BENDER. |
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Título: |
Isolamento de RNA para o estudo da expressão diferencial de genes durante a interação entre frutos de maçãs e Botryosphaeria dothidea. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FRUTICULTURA,18., 2004, Florianópolis. Tecnologia, competitividade, sustentabilidade: anais... Florianópolis: SBF, 2004. |
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Páginas: |
Não paginado. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
A maçã representa uma das mais importantes espécies de frutos de clima temperado comercializadas no mundo. Por outro lado, Botryosphaeria dothídea é o agente causal da podridão branca, uma das mais importantes doenças pós-colheita em maçãs. |
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Palavras-Chave: |
Doença d eplanta; Identificação genética. |
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Thesagro: |
Fruticultura; Fungo; Isolamento; Maçã; Podridão Branca; RNA. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/543749/1/ID11042.pdf
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Marc: |
LEADER 01170nam a2200265 a 4500
001 1543749
005 2022-08-22
008 2004 bl uuuu u00u1 u #d
100 1 $aOSTER, A. H.
245 $aIsolamento de RNA para o estudo da expressão diferencial de genes durante a interação entre frutos de maçãs e Botryosphaeria dothidea.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE FRUTICULTURA,18., 2004, Florianópolis. Tecnologia, competitividade, sustentabilidade: anais... Florianópolis: SBF$c2004
300 $aNão paginado.$c1 CD-ROM.
520 $aA maçã representa uma das mais importantes espécies de frutos de clima temperado comercializadas no mundo. Por outro lado, Botryosphaeria dothídea é o agente causal da podridão branca, uma das mais importantes doenças pós-colheita em maçãs.
650 $aFruticultura
650 $aFungo
650 $aIsolamento
650 $aMaçã
650 $aPodridão Branca
650 $aRNA
653 $aDoença d eplanta
653 $aIdentificação genética
700 1 $aMORAES, M. G. de
700 1 $aVALDEBENITO-SANHUEZA, R. M.
700 1 $aCORRENT, A.
700 1 $aBENDER, R. J.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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