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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia; Embrapa Milho e Sorgo. |
Data corrente: |
19/09/2017 |
Data da última atualização: |
15/02/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOUSA, C. A. F. de; PAIVA, D S. de; CASARI, R. A. das C. N.; MOLINARI, H. B. C.; OLIVEIRA, N. G. de; KOBAYASHI, A. K.; MAGALHAES, P. C.; GOMIDE, R. L.; SOUZA JUNIOR, M. T. |
Afiliação: |
CARLOS ANTONIO FERREIRA DE SOUSA, CNPAE; DAYANE SILVA DE PAIVA; RAPHAEL AUGUSTO DAS CHAGAS NOQUELI CASARI; HUGO BRUNO CORREA MOLINARI, CNPAE; NELSON GERALDO DE OLIVEIRA; ADILSON KENJI KOBAYASHI, CNPAE; PAULO CESAR MAGALHAES, CNPMS; REINALDO LUCIO GOMIDE, CNPMS; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
Título: |
A procedure for maize genotypes discrimination to drought by chlorophyll fluorescence imaging rapid light curves. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Plant Methods, v. 13, p. 1-17, 2017. |
DOI: |
10.1186/s13007-017-0209-z |
Idioma: |
Inglês |
Conteúdo: |
Background: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes to drought. However, unlike that, there is no need to perform daily and time consuming calibration routines. Moreover, plant acclimation to the dark is not required. The protocol can be applied to plants growing in both controlled conditions and full sunlight in the field. In addition, it generates parameters in a fast and accurate measurement process, which enables evaluating several plants in a short period of time. MenosBackground: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes... Mostrar Tudo |
Palavras-Chave: |
Phenotyping; Water deficit; Zea mays L. |
Thesaurus Nal: |
abiotic stress; corn; gas exchange; phenomics. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/168295/1/Procedure-maize.pdf
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Marc: |
LEADER 02834naa a2200313 a 4500 001 2081609 005 2018-02-15 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1186/s13007-017-0209-z$2DOI 100 1 $aSOUSA, C. A. F. de 245 $aA procedure for maize genotypes discrimination to drought by chlorophyll fluorescence imaging rapid light curves.$h[electronic resource] 260 $c2017 520 $aBackground: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes to drought. However, unlike that, there is no need to perform daily and time consuming calibration routines. Moreover, plant acclimation to the dark is not required. The protocol can be applied to plants growing in both controlled conditions and full sunlight in the field. In addition, it generates parameters in a fast and accurate measurement process, which enables evaluating several plants in a short period of time. 650 $aabiotic stress 650 $acorn 650 $agas exchange 650 $aphenomics 653 $aPhenotyping 653 $aWater deficit 653 $aZea mays L 700 1 $aPAIVA, D S. de 700 1 $aCASARI, R. A. das C. N. 700 1 $aMOLINARI, H. B. C. 700 1 $aOLIVEIRA, N. G. de 700 1 $aKOBAYASHI, A. K. 700 1 $aMAGALHAES, P. C. 700 1 $aGOMIDE, R. L. 700 1 $aSOUZA JUNIOR, M. T. 773 $tPlant Methods$gv. 13, p. 1-17, 2017.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria de Alimentos; Embrapa Mandioca e Fruticultura. |
Data corrente: |
21/12/2022 |
Data da última atualização: |
27/06/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SANTOS, C. S. DOS; SOUSA, M. B.; BRITO, A. C.; OLIVEIRA, L. A. de; CARVALHO, C. W. P. de; OLIVEIRA, E. J. de. |
Afiliação: |
CRISTIANO SILVA DOS SANTOS, UFRB; MASSAINE BANDEIRA SOUSA, UFRB; ANA CARLA BRITO; LUCIANA ALVES DE OLIVEIRA, CNPMF; CARLOS WANDERLEI PILER DE CARVALHO, CTAA; EDER JORGE DE OLIVEIRA, CNPMF. |
Título: |
Genome-wide association study of cassava starch paste properties. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
PLoS One , v. 17, n. 1, e0262888, 2022. |
ISSN: |
1932-6203 |
DOI: |
https://doi.org/10.1371/journal.pone.0262888 |
Idioma: |
Inglês |
Conteúdo: |
An understanding of cassava starch paste properties (CSPP) can contribute to the selection of clones with differentiated starches. This study aimed to identify genomic regions associated with CSPP using different genome-wide association study (GWAS) methods (MLM, MLMM, and Farm-CPU). The GWAS was performed using 23,078 single-nucleotide polymorphisms (SNPs). The rapid viscoanalyzer (RVA) parameters were pasting temperature (PastTemp), peak viscosity (PeakVisc), hot-paste viscosity (Hot-PVisc), cool-paste viscosty (Cold-PVisc), final viscosity (FinalVis), breakdown (BreDow), and setback (Setback). Broad phenotypic and molecular diversity was identified based on the genomic kinship matrix. The broad-sense heritability estimates (h2) ranged from moderate to high magnitudes (0.66 to 0.76). The linkage disequilibrium (LD) declined to between 0.3 and 2.0 Mb (r2<0.1) for most chromosomes, except chromosome 17, which exhibited an extensive LD. Thirteen SNPs were found to be significantly associated with CSPP, on chromosomes 3, 8, 17, and 18. Only the BreDow trait had no associated SNPs. The regional marker-trait associations on chromosome 18 indicate a LD block between 2907312 and 3567816 bp and that SNP S18_3081635 was associated with SetBack, FinalVis, and Cold-PVisc (all three GWAS methods) and with Hot-PVisc (MLM), indicating that this SNP can track these four traits simultaneously. The variance explained by the SNPs ranged from 0.13 to 0.18 for Set-Back, FinalVis, and Cold-PVisc and from 0.06 to 0.09 for PeakVisc and Hot-PVisc. The results indicated additive effects of the genetic control of Cold-PVisc, FinalVis, Hot-PVisc,and SetBack, especially on the large LD block on chromosome 18. One transcript encoding the glycosyl hydrolase family 35 enzymes on chromosome 17 and one encoding the mannose-p-dolichol utilization defect 1 protein on chromosome 18 were the most likely candidate genes for the regulation of CSPP. These results underline the potential for the assisted selection of high-value starches to improve cassava root quality through breeding programs. MenosAn understanding of cassava starch paste properties (CSPP) can contribute to the selection of clones with differentiated starches. This study aimed to identify genomic regions associated with CSPP using different genome-wide association study (GWAS) methods (MLM, MLMM, and Farm-CPU). The GWAS was performed using 23,078 single-nucleotide polymorphisms (SNPs). The rapid viscoanalyzer (RVA) parameters were pasting temperature (PastTemp), peak viscosity (PeakVisc), hot-paste viscosity (Hot-PVisc), cool-paste viscosty (Cold-PVisc), final viscosity (FinalVis), breakdown (BreDow), and setback (Setback). Broad phenotypic and molecular diversity was identified based on the genomic kinship matrix. The broad-sense heritability estimates (h2) ranged from moderate to high magnitudes (0.66 to 0.76). The linkage disequilibrium (LD) declined to between 0.3 and 2.0 Mb (r2<0.1) for most chromosomes, except chromosome 17, which exhibited an extensive LD. Thirteen SNPs were found to be significantly associated with CSPP, on chromosomes 3, 8, 17, and 18. Only the BreDow trait had no associated SNPs. The regional marker-trait associations on chromosome 18 indicate a LD block between 2907312 and 3567816 bp and that SNP S18_3081635 was associated with SetBack, FinalVis, and Cold-PVisc (all three GWAS methods) and with Hot-PVisc (MLM), indicating that this SNP can track these four traits simultaneously. The variance explained by the SNPs ranged from 0.13 to 0.18 for Set-Back, FinalVis, and Cold-PVis... Mostrar Tudo |
Thesagro: |
Fécula; Genoma; Mandioca. |
Thesaurus NAL: |
Cassava starch; Food paste; Genomics. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150198/1/pone.0262888.pdf
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Marc: |
LEADER 02860naa a2200277 a 4500 001 2150198 005 2023-06-27 008 2022 bl uuuu u00u1 u #d 022 $a1932-6203 024 7 $ahttps://doi.org/10.1371/journal.pone.0262888$2DOI 100 1 $aSANTOS, C. S. DOS 245 $aGenome-wide association study of cassava starch paste properties.$h[electronic resource] 260 $c2022 520 $aAn understanding of cassava starch paste properties (CSPP) can contribute to the selection of clones with differentiated starches. This study aimed to identify genomic regions associated with CSPP using different genome-wide association study (GWAS) methods (MLM, MLMM, and Farm-CPU). The GWAS was performed using 23,078 single-nucleotide polymorphisms (SNPs). The rapid viscoanalyzer (RVA) parameters were pasting temperature (PastTemp), peak viscosity (PeakVisc), hot-paste viscosity (Hot-PVisc), cool-paste viscosty (Cold-PVisc), final viscosity (FinalVis), breakdown (BreDow), and setback (Setback). Broad phenotypic and molecular diversity was identified based on the genomic kinship matrix. The broad-sense heritability estimates (h2) ranged from moderate to high magnitudes (0.66 to 0.76). The linkage disequilibrium (LD) declined to between 0.3 and 2.0 Mb (r2<0.1) for most chromosomes, except chromosome 17, which exhibited an extensive LD. Thirteen SNPs were found to be significantly associated with CSPP, on chromosomes 3, 8, 17, and 18. Only the BreDow trait had no associated SNPs. The regional marker-trait associations on chromosome 18 indicate a LD block between 2907312 and 3567816 bp and that SNP S18_3081635 was associated with SetBack, FinalVis, and Cold-PVisc (all three GWAS methods) and with Hot-PVisc (MLM), indicating that this SNP can track these four traits simultaneously. The variance explained by the SNPs ranged from 0.13 to 0.18 for Set-Back, FinalVis, and Cold-PVisc and from 0.06 to 0.09 for PeakVisc and Hot-PVisc. The results indicated additive effects of the genetic control of Cold-PVisc, FinalVis, Hot-PVisc,and SetBack, especially on the large LD block on chromosome 18. One transcript encoding the glycosyl hydrolase family 35 enzymes on chromosome 17 and one encoding the mannose-p-dolichol utilization defect 1 protein on chromosome 18 were the most likely candidate genes for the regulation of CSPP. These results underline the potential for the assisted selection of high-value starches to improve cassava root quality through breeding programs. 650 $aCassava starch 650 $aFood paste 650 $aGenomics 650 $aFécula 650 $aGenoma 650 $aMandioca 700 1 $aSOUSA, M. B. 700 1 $aBRITO, A. C. 700 1 $aOLIVEIRA, L. A. de 700 1 $aCARVALHO, C. W. P. de 700 1 $aOLIVEIRA, E. J. de 773 $tPLoS One$gv. 17, n. 1, e0262888, 2022.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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