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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
06/01/2020 |
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Data da última atualização: |
07/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAIVA, A. L. B.; MUDADU, M. de A.; PEREIRA, E. H. T.; MARRI, C. A.; GUERRA-DUARTE, C.; DINIZ, M. R. V. |
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Afiliação: |
ANA L. B. PAIVA, Fundação Ezequiel Dias; MAURICIO DE ALVARENGA MUDADU, CNPTIA; ELAINE H. T. PEREIRA; CAMILA A. MARRI; CLARA GUERRA-DUARTE; MARCELO R. V. DINIZ. |
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Título: |
Transcriptome analysis of the spider Phoneutria pertyi venom glands reveals novel venom components for the genus Phoneutria. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Toxicon, v. 163, p. 59-69, May 2019. |
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DOI: |
https://doi.org/10.1016/j.toxicon.2019.03.014 |
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Idioma: |
Inglês |
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Conteúdo: |
Phoneutria nigriventer spider venom has been studied for more than 40 years and several components with pharmacological potential have been described in it. However, studies on venoms from other species of the Phoneutria genus are scarce. In this work, a conventional cDNA library from the species Phoneutria pertyi venom glands was constructed, aiming to identify novel putative cysteine-rich peptide toxins for the genus Phoneutria. 296 unique sequences were identified and 51 sequences corresponded to putative cysteine-rich peptide toxins. Besides cysteine-rich peptide toxins, other putative venom components such as protease inhibitors, defensins and serine proteinases were identified. Furthermore, by manual curation of the sequences with no match at UniProt, we were able to identify glycine-rich proteins (GRP), a class of venom component never described in Phoneutria genus. This work describes the first complete sequences of toxins from the venom of P. pertyi and reveals that, despite most of the retrieved toxins show a high identity to toxins identified in Phoneutria genus, novel putative toxins remains to be described. |
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Palavras-Chave: |
Cysteine-rich toxin; Toxina rica em cisteína; Transcriptoma; Veneno de aranha; Venom glands. |
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Thesagro: |
Aranha. |
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Thesaurus Nal: |
Phoneutria nigriventer; Transcriptome. |
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Categoria do assunto: |
-- |
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Marc: |
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024 7 $ahttps://doi.org/10.1016/j.toxicon.2019.03.014$2DOI
100 1 $aPAIVA, A. L. B.
245 $aTranscriptome analysis of the spider Phoneutria pertyi venom glands reveals novel venom components for the genus Phoneutria.$h[electronic resource]
260 $c2019
520 $aPhoneutria nigriventer spider venom has been studied for more than 40 years and several components with pharmacological potential have been described in it. However, studies on venoms from other species of the Phoneutria genus are scarce. In this work, a conventional cDNA library from the species Phoneutria pertyi venom glands was constructed, aiming to identify novel putative cysteine-rich peptide toxins for the genus Phoneutria. 296 unique sequences were identified and 51 sequences corresponded to putative cysteine-rich peptide toxins. Besides cysteine-rich peptide toxins, other putative venom components such as protease inhibitors, defensins and serine proteinases were identified. Furthermore, by manual curation of the sequences with no match at UniProt, we were able to identify glycine-rich proteins (GRP), a class of venom component never described in Phoneutria genus. This work describes the first complete sequences of toxins from the venom of P. pertyi and reveals that, despite most of the retrieved toxins show a high identity to toxins identified in Phoneutria genus, novel putative toxins remains to be described.
650 $aPhoneutria nigriventer
650 $aTranscriptome
650 $aAranha
653 $aCysteine-rich toxin
653 $aToxina rica em cisteína
653 $aTranscriptoma
653 $aVeneno de aranha
653 $aVenom glands
700 1 $aMUDADU, M. de A.
700 1 $aPEREIRA, E. H. T.
700 1 $aMARRI, C. A.
700 1 $aGUERRA-DUARTE, C.
700 1 $aDINIZ, M. R. V.
773 $tToxicon$gv. 163, p. 59-69, May 2019.
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