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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
03/09/2021 |
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Data da última atualização: |
03/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAES, B. G.; STEINDORF, A. S.; FORMIGHIERI, E. F.; PEREIRA, I. S.; ALMEIDA, J. R. M. de. |
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Afiliação: |
BARBARA G. PAES, Universidade de Brasília; ANDREI STECCA STEINDORF, Universidade de Brasília; EDUARDO FERNANDES FORMIGHIERI, CNPAE; ILDINETE SILVA PEREIRA, Universidade de Brasília; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
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Título: |
Physiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
AMB Express, v. 11, n. 2, 2021. |
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Descrição Física: |
PDF: il. color. |
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DOI: |
https://doi.org/10.1186/s13568-020-01170-9 |
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Idioma: |
Inglês |
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Conteúdo: |
The negative efects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a signifcant drawback to the usage of biomass feedstocks for the production of fuels and chemicals. The yeast Pichia pastoris has shown a great biotechnological potential for producing heterologous proteins and renewable chemicals. Despite its relevance, the performance of P. pastoris in presence of lignocellulose-derived inhibitors remains unclear. In this work, our results show for the frst time the dose-dependent response of P. pastoris to acetic acid, furaldehydes (HMF and furfural), and sugarcane biomass hydrolysate, both at physiological and transcriptional levels. The yeast was able to grow in synthetic media with up to 6 g.L-1 acetic acid, 1.75 g.L-1 furaldehydes or hydrolysate diluted to 10% (v/v). However, its metabolism was completely hindered in presence of hydrolysate diluted to 30% (v/v). Additionally, the yeast was capable to co-consume acetic acid and glucose. At the transcriptional level, P. pastoris response to lignocellulose-derived inhibitors relays on the up-regulation of genes related to transmembrane transport, oxidoreductase activities, RNA processing, and the repression of pathways related to biosynthetic processes and central carbon metabolism. These results demonstrate a polygenetic response that involves detoxifcation activi ties, and maintenance of energy and cellular homeostasis. In this context, ALD4, OYE3, QOR2, NTL100, YCT1, and PPR1 were identifed as target genes to improve P. pastoris tolerance. Altogether, this work provides valuable insights into the P. pastoris stress tolerance, which can be useful to expand its use in diferent bioprocesses. MenosThe negative efects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a signifcant drawback to the usage of biomass feedstocks for the production of fuels and chemicals. The yeast Pichia pastoris has shown a great biotechnological potential for producing heterologous proteins and renewable chemicals. Despite its relevance, the performance of P. pastoris in presence of lignocellulose-derived inhibitors remains unclear. In this work, our results show for the frst time the dose-dependent response of P. pastoris to acetic acid, furaldehydes (HMF and furfural), and sugarcane biomass hydrolysate, both at physiological and transcriptional levels. The yeast was able to grow in synthetic media with up to 6 g.L-1 acetic acid, 1.75 g.L-1 furaldehydes or hydrolysate diluted to 10% (v/v). However, its metabolism was completely hindered in presence of hydrolysate diluted to 30% (v/v). Additionally, the yeast was capable to co-consume acetic acid and glucose. At the transcriptional level, P. pastoris response to lignocellulose-derived inhibitors relays on the up-regulation of genes related to transmembrane transport, oxidoreductase activities, RNA processing, and the repression of pathways related to biosynthetic processes and central carbon metabolism. These results demonstrate a polygenetic response that involves detoxifcation activi ties, and maintenance of energy and cellular homeostasis. In this context, ALD4, OYE3, QOR2, NTL1... Mostrar Tudo |
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Palavras-Chave: |
Furaldehydes; Komagataella phaffi; Lignocellulosic hydrolysate. |
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Thesaurus Nal: |
Acetic acid; Pichia pastoris; Stress tolerance. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/225706/1/Physiological-characterization-and-transcriptome-analysis-2021.pdf
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Marc: |
LEADER 02579naa a2200265 a 4500
001 2134061
005 2021-09-03
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s13568-020-01170-9$2DOI
100 1 $aPAES, B. G.
245 $aPhysiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors.$h[electronic resource]
260 $c2021
300 $cPDF: il. color.
520 $aThe negative efects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a signifcant drawback to the usage of biomass feedstocks for the production of fuels and chemicals. The yeast Pichia pastoris has shown a great biotechnological potential for producing heterologous proteins and renewable chemicals. Despite its relevance, the performance of P. pastoris in presence of lignocellulose-derived inhibitors remains unclear. In this work, our results show for the frst time the dose-dependent response of P. pastoris to acetic acid, furaldehydes (HMF and furfural), and sugarcane biomass hydrolysate, both at physiological and transcriptional levels. The yeast was able to grow in synthetic media with up to 6 g.L-1 acetic acid, 1.75 g.L-1 furaldehydes or hydrolysate diluted to 10% (v/v). However, its metabolism was completely hindered in presence of hydrolysate diluted to 30% (v/v). Additionally, the yeast was capable to co-consume acetic acid and glucose. At the transcriptional level, P. pastoris response to lignocellulose-derived inhibitors relays on the up-regulation of genes related to transmembrane transport, oxidoreductase activities, RNA processing, and the repression of pathways related to biosynthetic processes and central carbon metabolism. These results demonstrate a polygenetic response that involves detoxifcation activi ties, and maintenance of energy and cellular homeostasis. In this context, ALD4, OYE3, QOR2, NTL100, YCT1, and PPR1 were identifed as target genes to improve P. pastoris tolerance. Altogether, this work provides valuable insights into the P. pastoris stress tolerance, which can be useful to expand its use in diferent bioprocesses.
650 $aAcetic acid
650 $aPichia pastoris
650 $aStress tolerance
653 $aFuraldehydes
653 $aKomagataella phaffi
653 $aLignocellulosic hydrolysate
700 1 $aSTEINDORF, A. S.
700 1 $aFORMIGHIERI, E. F.
700 1 $aPEREIRA, I. S.
700 1 $aALMEIDA, J. R. M. de
773 $tAMB Express$gv. 11, n. 2, 2021.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| Registros recuperados : 15 | |
| 11. |  | PEDROSA, M. J.; FERREIRA, L. M. M.; PAES, B. G.; NAKAI, D. K.; ALMEIDA, J. R. M. de. Efeito da superexpressão do fator de transcrição HAA1 em Komagataella phaffii produtora de ácido xilônico. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 7., 2023, Brasília, DF. Anais... Brasília, DF: Embrapa, 2023. p. 113-117.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 12. | | PESSOA, R. R.; PAES, B. G.; ANTUNES, A. C. S.; ALVES, N.; FRANCO, P. F.; ALMEIDA, J. R. M. de. Improved yeasts for production of fuels and chemicals from renewable resources. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 36., 2014, Clearwater Beach, FL. [Abstracts ...]. Fairfax: Society for Industrial Microbiology and Biotechnology, 2014. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 14. | | ANTUNES, A. C. S.; PESSOA, R. R.; PAES, B. G.; SIFUENTES, D. N.; ALMEIDA, J. R. M. de; FRANCO, P. F. Screening and genetic improvement of microorganisms for production of fuels and chemical. In: SEMINÁRIO BRASILEIRO DE TECNOLOGIA ENZIMÁTICA - ENZITEC, 11., 2014, Rio de Janeiro, RJ. [Para um mundo sustentável: resumos...]. [S.l: s.n], 2014. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 15. | | ADEGAS, F. S.; GAZZIERO, D. L. P.; BONANI, J. C.; PRECINOTTO, C. V.; GARBIATE, M. V.; PAES, B. L.; ASSIS, D. N. de; OLIVEIRA JUNIOR, R. S. de. Novo caso de resistência de planta daninha ao glifosato no Brasil: picão-preto (Bidens subalternans). Londrina: Embrapa Soja, 2023. 6 p. (Embrapa Soja. Comunicado Técnico, 107).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 15 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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